选择标记

植物选择标记基因hpt在大肠杆菌中的融合表达、纯化及活性测定

Fusion Expression , Purification and Bioactivity Assay of Plant Selectable Marker Gene hpt in E. coli

对小麦遗传转化上常用的选择标记基因NPTⅡ的选择剂及GUS基因在幼胚愈伤组织中的转移进行了研究。

The selectable marker gene ( NPT ⅱ ) that is usually used for wheat transformation was tested for the optimal selector .

无抗性选择标记的植酸酶基因转化DNA的构建

Construction of transformed system for phytase gene without resistance label

以Bar为选择标记的遗传转化体系的建立

Development of Transformation System with Selective Marker Bar Gene

目前，正结合进行田间分离纯合和DNA分子鉴定，培育去除选择标记基因的转基因抗虫玉米自交系。

At present , inbred lines of transgenic insect resistant maize with selective marker gene removed are in separating and inbreeding program assisted by DNA marker detection .

利用DNA免疫法制备植物选择标记基因hpt表达蛋白抗体的研究

Study on the preparing of polyclonal antibodies against plant-selected maker gene hpt expression protein by DNA immunization

用BPV转化灶做选择标记表达HBsAg的研究

Using BPV Transforming Foci as Selective Markers to Express HBsAg

含kan抗性基因作为选择标记。

Kanmycin resistance gene was used as a selective marker .

用hMT-Ia基因作选择标记基因在BPV载体中表达HBsAg的研究

Using bMT-Ia Gene as Selective Marker to Express HBsAg in a BPV Vector

无抗性选择标记的转高赖氨酸蛋白（LRP）基因籼稻恢复系的获得

LRP Transgenic Indica Rice Restorer Line without Resistance Selection Marker

以neo作选择标记富集和筛选阳性重组杆状病毒

An efficient method to identify positive recombinant baculovirus by using Neo as selection marker

利用双T-DNA载体系统获得无选择标记转基因菊花

Obtaining Marker Free Transgenic Chrysanthemum Through Double T-DNA System

循环中首先选择标记名为content的所有子元素，然后从发现的节点中获得CDATA文本。

This first selects all of the children with the tag name content , then gets the CDATA text from within the found nodes .

结果经脂质体法转染CHO细胞，以氨甲喋呤为选择标记，经过一轮扩增后，获得表达绿色荧光蛋白的CHO细胞株。

Results After one cycle of amplification by methotrexate ( Mtx ), the CHO cell colonies stably expressed green fluorescent protein .

通过共转化法培育无抗性选择标记的转反义Wx基因水稻的研究

Co-transformation of Rice for Production of Selectable Marker-free Transgenic Plants Containing Antisense Wx Gene

同时构建了一个双T-DNA载体建立了一个无选择标记转化体系，用于遗传转化研究。

Also , we build a marker-free transforming system by using double T-DNAs vector for genetic transformation .

经Cre介导后可将外显子1α和选择标记Neo基因同时删除。

Both exon 1 α and the selection marker Neo will be deleted in targeted cells when mediated by Cre .

Bar基因是转基因商品化作物中应用最多的目标基因，也是水稻遗传转化中应用较多的选择标记基因，因此，建立以Bar基因为选择标记的通用和高效的遗传转化体系非常必要。

Bar gene is widely used in commercialized transgenic crops as a selective marker and also used in rice transformation . Therefore , the establishment of a kind of common and efficiency genetic transformation system is necessary .

研究表明，两段独立的T-DNA在一定的条件下共同转化植物时，可能整合到植物基因组的不同染色体上，转基因植株在自交后代发生分离，可得到无选择标记的转基因株系。

Two independent T-DNA segments could integrate into different sites of plant genome when co-transformation , and marker-free plants could be obtained .

无抗性选择标记转AP1基因抗病水稻新品系的选育

Breeding of Selectable Marker-Free Transgenic Rice Lines Containing AP1 Gene with Enhanced Disease Resistance

为彻底消除选择标记基因的负面影响，目前已经发展了多种去除选择标记基因的植物转化系统，如共转化系统、位点特异性重组系统、转座子系统及MAT载体系统等。

Presently many plant transformation systems have been developed in order to remove selectable marker genes such as co-transformation system , site-specific recombination system , transposable element system and MAT vector system etc.

在对T1代群体进行叶片涂抹除草剂检测的基础上，不抗除草剂植株进行PCR、Southernblot和NorthernBlot检测，共鉴定出41棵无选择标记转基因植株，无标记植株获得率为7·6%。

By the analysis of PCR , southern blot and northern blot combining with leaf painting of herbicide in T_1 progenies , 41 plants were confirmed to be eliminated of bar gene with the frequency of 7.6 % .

进一步证明上述利用暂时选择标记k1l基因构建重组MVA的系统具有十分可靠的安全性，适合作为人体活疫苗开发和基因治疗的载体。

Our data indicated that recombinant MVA with a transient selection marker system was safe for use as live vaccine and gene therapy vector for human .

在此基础上，找到了合适的Basta筛选压，为以bar基因作为选择标记基因的水稻遗传转化奠定了基础。

On the basis of this result , optimal selection pressure of basta was determined , thus laying a foundation for the transformation of rice with bar gene serving as selection marker gene .

目的为了验证CodA基因是否适宜作为有效的青蒿基因打靶负选择标记。

Objective To explore the feasibility of utilizing the cytosine deaminase A ( CodA ) gene as an effective negative selectable marker in Artemisia annua for gene targeting .

同时，还发现，gypsy绝缘子能增强选择标记基因的表达，从而有利于转基因植株的筛选。

The gypsy insulator was also able to improve the expression of a selectable marker gene outside the insulated region , which facilitated the screen of transgenic plants .

目的：制备抗潮霉素B磷酸转移酶（HPT）的单克隆抗体（McAb），建立一种快速检测转基因作物中该选择标记基因HPT编码蛋白的方法。

Objective : To produce monoclonal antibodies ( McAb ) against hygromycin B phosphotransferase ( HPT ) and to establish a rapid method for the measurement of HPT antigen in the genetically modified crops ( GMC ) .

用产黄青霉HY876作受体菌，建立amds（Acetamidase）基因为选择标记的VHb表达系统。

A exogenous gene expression system was established in P. chrysogenum strain by using acetamidase ( amds ) gene as selection markers .

分析了T0代发生共转化的13个T1代株系，共951株植株，分离得到只具有sck基因，无选择标记基因的植株为166株，其比率为17.46%。

The percentage of co-transformation with hpt and sck were 63.93 % . 166 marker free transgenic plants were obtained by PCR from 13 of lines which developed from TO plants .

在MS培养基中用0.5μg/mL的PPT可以代替卡那霉素对转化后代进行筛选，这暗示NADPGDH基因可以作为一种新的选择标记用于植物基因工程的研究。

0.5 μ g / mL PPT could be used as a selecting drug instead of kanamycin to develop the transformants . These results suggested that the NADP_GDH gene might be used as a new selecting gene in the future research of plant gene engineering .