转化子

应用微生物形态学和RAPD技术，对转化子的遗传背景进行鉴定。

With microbe morphologic and RAPD technique , we made a fringe identification of inherited background of the transformant .

枯草芽孢杆菌proA基因突变对提高大肠杆菌转化子渗透压耐受能力的影响

Influence of the Mutated proA Gene from Bacillus subtilis on Improving Osmotic Tolerance of Escherichia coli Transformant

将转化子进行PCR筛选，得到酵母重组菌株。

The recombinants were obtained by the assay of PCR .

目的基因特异引物PCR、潮霉素抗性测定对转化子进行了验证。

Transformants were verified by PCR of lys gene and hygromycin resistant .

经PCR检测发现，在8个转化子中有6个转化子获得特异性扩增条带。

PCR analysis showed that 6 of 8 transformants obtained special amplification band .

通过表型观察和PCR扩增筛选产生的互补转化子。

The resulting rescued transformants were screened by phenotype characterization and PCR amplification .

对所获得的部分转化子进行PCR及Southernblot验证。

Parts of transformants were screened by PCR amplification and Southern blot .

抗性转化子的Southern分析表明，潮霉素磷酸转移酶基因已整合到受体黑曲霉的基因组DNA中。

Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant .

对转化子进行酶活测定，酶活数据结果表明GOD基因在重组菌中得到了分泌表达。

Then determination of enzyme activity , enzyme activity data showed that GOD gene in recombinant Aspergillus niger has been secreted expression .

经PCR检测得到了5个转化子，以菌丝和原生质体为转化受体的PCR阳性率分别为14%和50%。

Obtained 5 transformants detected by PCR , and the PCR positive rates were 14 % , 15 % . 5 .

其他三株过表达转化子与G20相比，其三萜含量均有明显的提高。

The triterpenes content of the other three over-expression transformants were also significantly increased .

采用SDS-PAGE蛋白电泳技术，检测BL21-pET-28a-CTX-M-38转化子表达产物。

The expressing products of CTX-M-38 transformant was detected by SDS-PAGE electrophoresis technique . 5 .

采用的基因克隆策略是用Southern杂交对其邻近基因进行定位后构建基因组文库，再用斑点杂交从基因文库中筛选目的转化子。

The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southern blot and to screen the aim transformant by dot blotting .

PCR检测HPH基因表达盒，结果显示被测转化子基因组中均成功整合了目的片段。

The presence of the fragment of hph gene casket in the transformants was checked by PCR .

随机取15个转化子进行点渍法（Dottingblotting）检验，结果均呈阳性。

15 randomly selected transformants expressed positive by dotting blotting test .

通过转化子基因组DNAPCR和斑点杂交检测，外源EGFP基因存在于转化子染色体上。

The strain was examined by genomic DNA PCR and spot blotting , and the transformed EGFP gene lied in chromosome of P. aeruginosa .

Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.Pastoris染色体中。

It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing .

通过对转化子的PCR检测和Southern杂交分析表明，TDNA已整合进毛壳菌基因组中，而且在所检测的转化子中都是以单拷贝的形式整合，转化子都能够稳定遗传。

PCR and Southern analysis showed that the T-DNA was integrated into the genome . Among of the examined transformants contained a single copy , and was stable through mitotic cell division .

转化子的潮霉素抗性可达到200μg/mL，GUS检测阳性。

The hygromycin resistance of transformants reached 200 μ g / mL and the GUS activity was tested positive .

SDS-PAGE蛋白电泳后，各泳道酶蛋白量多少依次为包涵体蛋白、全菌蛋白以及培养液上清，提示转化子表达产物主要存在于包涵体内。

The SDS-PAGE electrophoresis results showed that the expressing products quantity was supernatant , whole bacterial proteins and inclusion body protein in turn .

2提取1～10号转化子的DNA，根据T-DNA上已知Gus基因序列设计引物，进行PCR。

To identify the transformants , genomic DNA of transformants MZ1 ~ 10 were . identified by PCR with primers designed according to Gus gene sequences of T-DNA .

将阳性转化子菌株进行表型和RNA转录水平验证，表明hph基因整合入平菇的基因组，其抗性可以稳定的遗传和表达，从而建立了平菇的遗传转化体系。

It was proved that the hph gene integrated into the genome and expressed stability . Thereby , we established a genetic transformation system of Pleurotus ostreatus .

同时，发现电击后的细胞必需在高渗培养基中才能存活，电击后2～3h的复苏表达期和用亚抑制抗生素浓度选择转化子，这些对电转化成功以及提高转化效率都是十分关键的。

A post pulse recovery time of 2 ~ 3h and the use of sub inhibitory concentrations of antibiotics in the selective plates were very critical for electrotransformation .

结果质粒电泳图谱显示，转化子DH5α所含质粒与肺炎克雷伯菌所含质粒相同，电转化取得成功；

Results Electropherogram showed that the plasmids in DH5 α were the same as those in Klebsiella pneumonia and electroporation was successful ;

转化子经过表型鉴定，PCR分析和G418浓度梯度筛选获得了高拷贝的重组子（pas-01）。

A high-copy recombinant ( pas-01 ) was screened out from 30 positive transformants by phenotypic identification , PCR analysis and G418 concentration gradient selection .

为了获得具有防病和解磷双重功能的木霉菌REMI转化子。

Objective The aim of this study was to obtain Trichoderma REMI transformant with double functions of disease prevention and phosphate-dissolving .

随机挑取48个白色菌落，经过ColonyPCR，其中有1个转化子的扩增产物为双带，其余均为0.2~2kb之间的单一条带。

Forty-eight white colonies were randomly picked and their inserts were colony PCR amplified . The PCR product from one of the colonies contained two inserts ; the others contained single insert , having a size of 0.2 kb to 2 kb .

平衡期初期的受体菌用溶菌酶和溶葡萄球菌素处理，所获得的原生质体与质粒DNA混合，以PEG诱导，涂布于含药的双层DM3再生培养基上，即可获得Tc~r或Cm~r转化子。

Protoplasts were prepared by treating recipients at early stationary phase with lysozyme and lysostaphin . Mixed protoplasts and DNA , then treated with polyethylene glycol ( molecular weight . 6000 ), Tc ~ r or Cm ~ r transformants were obtained .

人sCR1酵母分泌型表达载体构建及其高拷贝转化子的快速筛选

Construction of human sCR1 ferment cell secreting type carrier and rapid screen for its high copy carrier

用MM和MD平板分离His+Mut+型菌落，YPD平板筛选出G418高抗性的His+Mut+转化子。

Colony with phenotype His + Mut + was isolated by using MD and MM plates and His + Mut + transformant with high resistance to G418 was screened out by using YPD plate .