翻译后加工

每个拷贝N端前两位氨基酸序列为KR，是两栖动物典型的翻译后加工位点。

From N-terminal , the first two amino acids residues of each copy are K-R , it is classic site of post-translational processing in amphibian .

选用的巴氏毕赤酵母不但具有原核生物易培养、操作便利等特点，又具有真核生物蛋白翻译后加工的能力；同时具有高表达、高稳定和高分泌等优点。

P. pastoris is easy to culture and convenient to manipulate , and its post-translational processing capability of eukaryotic proteins is also attractive ; furthermore it has interesting characteristics of high expression , high stability and high secretion .

α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶（α-AE或PAM）催化完成，是酰胺化多肽生物合成途径中的限述步骤。

The a-amide comes from posttranslational modification that is catalyzed by α - amidase (α - AE or PAM ) . The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides .

加工蛋白酶[负责翻译后加工]给一个作品作最后加工

Processing protease put the finishing touches to a piece of work

蛋白质肽链的翻译后加工

Studying the processing of polypeptide chain in protein translation and post-translation

活血化瘀中药对人黑素瘤细胞酪氨酸酶翻译后加工的影响

Effects of Blood Activating Chinese Herbs on Processing of Tyrosinase in Human Melanoma Cells

5酪氨酸酶翻译后加工和糖基化。

Posttranslational processing and glycosylation of tyrosinase .

目的探讨中药乙醇提取物对酪氨酸酶翻译后加工成熟及运输的影响。

Objective To explore the effects of alcoholic extracts of traditional Chinese medicines on the post-translational processing and trafficking of tyrosinase .

在大多数情况下，在昆虫细胞中表达的真核蛋白质的翻译后加工过程与其在哺乳动物细胞中的方法类似。

In most cases , posttranslational processing of eukaryotic proteins expressed in insect cells is similar to protein processing in mammalian cells .

而原核表达系统由于没有翻译后加工修饰系统，表达的真核细胞蛋白质多以包涵体形式表达，复性纯化方法复杂。

The prokaryotic expression system has no post-translational modification system and the exogenous proteins always emerge in the inclusion body , complicating the course of renaturation and purification .

杆状病毒昆虫细胞表达系统是逐渐发展起来，并且在现阶段得到广泛应用的真核表达系统，它具有蛋白翻译后加工修饰的功能，从而能够形成与天然蛋白高度一致并且可溶的蛋白。

Baculovirus expression vector system is an eukaryotic expression system which was gradually developed , and widely used . It has the function of modification , thus forming the protein highly similar to the natural and soluble .

该系统具有对蛋白质完整的翻译后加工能力，包括糖基化、磷酸化、信号肽切除及肽段的切割和分解等，修饰的位点与天然蛋白在细胞内的情况一致。

It has the processing capacity of co-translation , including the glycosylation , the phosphorylation , the excision of signal peptide , the cutting and decomposition polypeptide , and its modified sites are the same as ones of natural proteins in cells .

真核蛋白往往需要翻译后的加工修饰才能具有完整的生物活性。

Expression of eukaryotic protein often require modification after translation process to get a complete biological activity of protein .

蛋白质剪接是蛋白质内含肽介导的，一种在蛋白质水平上翻译后的加工过程，它由一系列分子内的剪切-连接反应组成。

Protein splicing , which is an intein mediated posttranslational processing , involves a series of intramolecular cleavage - ligation reactions .

尽管研究者已在原核细胞表达系统对胸腺肽的表达进行了研究，但由于原核表达系统缺乏翻译后的加工修饰等缺点，限制了其应用。

Although the thymosin has been successfully expressed in the prokaryotic cell system , its application is restricted because of its deficiency of modification after translation .

丝状真菌作为微生物具有结构简单、生长迅速、操作简便的优点，同时作为低等真核生物，又具有真核基因表达机器和真核蛋白质翻译后修饰加工装置，具有与哺乳动物系统相似的蛋白修饰性能。

Filamentous fungus has the advantages of simple structure and fast growth , so it is easy to manipulate and culture . Being a kind of lower eukaryotes , it also has eukaryotic protein modification apparatus and the post translation modification mechanism , which is similar to that of mammals .

转录、翻译以及翻译后加工各个环节对内质网应激时基因的表达产生调控，且方式各不相同。

Transcription , translation and post-translational processing and modification contribute to the regulation of endoplasmic reticulum stress response genes ( ERSRGs ) expression and the manners vary with different processes .

基因转录水平的研究只能在一定程度上反映基因表达产物的变化，而真正发挥功能的蛋白质需要经过转录后加工、翻译调控以及翻译后加工等许多步骤和调控才能形成。

Transcription levels only to a certain extent reflect the changes in product of gene expression , but the real function of the protein is formed , which need go through the steps of post-transcriptional processing , translation regulation and post-translational processing and regulation and so on .