构建融合蛋白

PCR一步法构建融合蛋白基因fpg

Construction of a Fused fpg Gene by Using TP-PCR Method

目的克隆淋球菌孔蛋白PIB和PIA基因并构建融合蛋白真核表达质粒pCI-PIB-PIA，了解pCI-PIB-PIA能否被小鼠骨骼肌细胞摄取并表达。

Objective Clone the genes of porin PIB and PIA of Gonococcus . To construct a recombinant eukaryotic expression vector containing both porin gene PIB and PIA , and to confirm the expression of pCI-PIB-PIA in muscule cells .

介绍了构建融合蛋白的策略，优点及其存在的不足。

The strategies of fusion protein designing and its advantages and disadvantages in the bioprocessing of recombinant proteins , were introduced in this article .

克隆人胰腺组织激肽释放酶基因（hKK），构建融合荧光蛋白基因的真核表达载体，在CHO细胞中表达，为开发激肽释放酶基因工程产品以及开展基因治疗高血压研究奠定了基础。

Molecular cloning and expression of human tissue kallikrein gene fused with EGFP report gene in CHO cells for the development of human kallikrein products and further study in gene therapy .

采用一种不需要限制核酸酶和连接酶的新方法&“PCR一步法”将芳香烃化合物降解的关键基因pheB和绿色荧光蛋白编码基因gfp融合，构建得到融合蛋白基因fpg。

TP-PCR , a method developed for fusion gene construction without the use of endonuclease and ligase , was performed to construct a fused fpg gene .

Oleosin与GUS融合蛋白基因的植物表达载体的构建及融合蛋白性质的预测

The Construction of Plant Expression Vector p ~ ( BI-LEA-O : : G121 ) of Oleosin and GUS Fusion Protein Gene and the Forecast of Its Properties

目的：为用基因工程的方法人工表达芳香烃受体（AhR）及芳香烃受体核转运蛋白（ARNT），构建了融合蛋白的表达载体。

Objective : In order to get the proteins of AhR and ARNT by genetic engineering , prokaryotic expression vectors were constructed .

并以cMyc标记肽为例，简述如何构建用于融合蛋白表达的植物表达载体，及如何利用标记肽及其特异性抗体表达并纯化融合蛋白质。

And take c | myc as example , it shows how to construct the eukaryotic expression vector which is used to express the fused protein , and how to detect and purify the fused protein by epitope tagging and its specific monoclonal antibody .

目的克隆大鼠AQP4基因并构建其融合蛋白表达载体，以便载体在体内进行AQP4基因干预。

Objective To clone AQP4 gene and construct its expression vector in order to study the function of AQP4 in cerebral water metabolism in the rat by genetic intervention .

小鼠脂多糖应答蛋白mlrpS的载体构建及融合蛋白表达

Construction of Expression Vector of mlrpS , A Mouse Protein of Lipopolysaccharide Response and Expression of mlrpS Fusion Protein

结论应用基因工程技术，成功地构建了融合蛋白EGF-linker-TCS的表达载体，表达并纯化了该融合蛋白，为进一步研究其结构功能关系和肿瘤临床应用奠定了基础。

Conclusion The recombinant plasmid PQE30 / EGF-linker-TCS has been successfully constructed , which provides a basis for further structural and functional study of EGF and TCS and their potential clinical application for cancer therapy .

构建表达融合蛋白的重组质粒和对照质粒。

To construct the prokaryotic expression vector and the control vector .

人β-防御素-3基因定点突变，原核表达载体构建和融合蛋白表达

Site-directed mutation and prokaryotic expression vector construction and fusion protein expression of human beta-defensin 3

结论：成功表达纯化并鉴定构建了ESAT-6融合蛋白。

Conclusion : The ESAT-6 fusion protein was successfully expressed and purified , identified .

目的构建重组DT/bFGF融合蛋白表达载体，制备高纯度的DLF融合毒素。

Objective To construct the expression vector for DT / bFGF fusion protein and prepare highly purified DLF fusion toxin .

构建了lovE-His融合蛋白原核重组表达载体，并高效表达和纯化了该融合蛋白，为进一步深入了解该类转录因子生物学功能和作用机理奠定了实验基础。

Construction of prokaryotic recombinant expression vector pET – lovE , expression and purification of the fusion protein with high efficiency provide the experiment basis for further understanding of biological function and mechanism of this kind of transcription factor .

通过设计引物进行PCR扩增的方式直接将蛋白转导结构域HIVTAT基因连接至G1F/M2基因的上游，构建新的重组融合蛋白TAT-G1F/M2。

By primers design and PCR amplification , the gene of protein transduction domain HIV TAT was connected to the upstream of gene G1F / M2 . A new recombinant protein expression vector , termed pET-TAT-G1F / M2 , was constructed .

为了构建力达霉素融合蛋白表达技术平台，我们还开展了RGD-LDM融合蛋白的研究工作。

To construct the LDM fusion protein expression technical platform , preparing RGD-LDM fusion protein was also carried out .

二是将肿瘤坏死因子与其它蛋白共同构建双功能的融合蛋白；

The construction of bi-function fusion proteins with other proteins ;

β分泌酶原核表达载体的构建、表达及融合蛋白纯化

Construction , expression of β secretase prokaryotic expression vector and fusion protein purification

还探索了转录终止序列对融合基因蛋白表达水平的影响，构建了高效表达融合蛋白的载体－宿主系统。

Based on the results , a vector-host system was established for the high expression of fusion proteins .