瞬时表达

生长抑素DNA疫苗在COS细胞中的瞬时表达

Transient Expression of Somatostatin DNA Vaccine in COS Cells

CEA真核表达载体的瞬时表达及检测

Transient expression of eukaryotic expression plasmid CEA in vitro and its detection

抗马铃薯Y病毒RNA干扰载体的构建及瞬时表达分析

Construction and Agroinfiltration Analysis of RNA Inference Vectors Against Potato Virus Y

MCF-7细胞中组织型纤溶酶原激活剂乳腺特异性表达载体的瞬时表达

Transient expression of tissue-type plasminogen activator in mammary carcinoma cell line MCF-7

GUS基因在坛紫菜叶状体体细胞内的瞬时表达；

GUS gene transient expression within the P. haitanensis thallus somatic cells ;

苜蓿愈伤组织诱导及GUS基因瞬时表达

Inducing of Alfalfa calli and transient expression of GUS gene in Calli

但在瞬时表达中，mars对gus基因的表达没有影响。

But Mars had no effect on the GUS gene expression levels in transient expression .

GUS基因在烟草原生质体中的瞬时表达

Transient expression of GUS gene in tobacco protoplasts

通过对基因枪介导法转化的烟草叶片及洋葱表皮细胞中GFP基因的瞬时表达测定表明，这一表达系统在光照条件下可显著增强GFP基因的表达。

The results indicated that this expression system has strengthened the transient expression of GFP .

柑橘原生质体瞬时表达体系的建立及默科特橘橙转GFP种质的创造

Establishment of Citrus Protoplast Transient Expression System and Creation of GFP Transgenic Murcott Tangor Germplasm

利用REF启动子构建橡胶树乳管特异表达载体及GUS基因的瞬时表达

Construction of Hevea brasiliensis Transient Expression Vector with REF Promoter

结果表明，瞬时表达的siRNA能够特异性干扰TMV侵染。

Our results showed that transiently expressed siRNA could specifically interfere with TMV infection .

汉坦病毒A9株M基因片段全长cDNA克隆及其在痘苗病毒中的瞬时表达

Cloning and Transient Expression of M Genome Segment cDNA of Hantaan Virus Chinese Isolate A9

组织化学染色结果表明：GUS基因的瞬时表达率可以达到70%以上。

Histochemical staining showed the temporary expression rate of GUS gene reached more than 70 % .

轰击次数多少对GUS基因瞬时表达影响不明显。

The bombarding frequency did not show significant influence on the transient expression of GUS gene .

结论反义基因的导入在瞬时表达时可影响细胞中靶蛋白及其他生长相关蛋白的表达，但对细胞周期和DNA合成的影响尚小。

Conclusion Transient expression of the transfected antisense c-myc molecules can change the expression of cellular target proteins and other growth-related proteins , but has little effect on cell cycle and DNA synthesis .

t-PA突变体乳腺表达载体的构建及其在乳腺中的瞬时表达

Construction of mutant t-PA mammary gland expression vector and transient expression in mammary gland tissues

利用细胞瞬时表达实验在HeLa细胞系中研究CTCF各结构域的转录活性。

To know the transcription activities of CTCF domains in HeLa cells .

抗氧化剂对农杆菌介导的大豆下胚轴GUS基因瞬时表达的影响

The effects of antioxidants on the transient expression of GUS gene in Soybean Hypocotyls mediated by Agrobacterium tumefaciens

将启动子片段分别以不同方向与gus报告基因和nos终止子融合，构建了瞬时表达载体。

Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation .

Rubisco小亚基基因片段的亚克隆及其瞬时表达载体的构建

Subcloning of Rubisco Small Subunit Gene Fragment and the Construction of Transient Expression Vector

利用GUS瞬时表达探讨香石竹品种云红二号基因枪转化参数

Transformation Parameters of Carnation " Yunhong 2 " by Particle Bombardment in virtue of Transient Expression of GUS Gene

方法用带有GFP突变体的质粒转染到表达细胞，观察GFP瞬时表达的情况：1直接测定GFP在COS-7细胞中的表达并观察表达的稳定性；

Methods The plasmids carrying mutated GFP gene were transfected into the eukaryotic cells to observe transient gene expression .

因此采用RAPD技术，可以从当代DNA分子水平的变化检测诱变效率。瞬时表达比较马铃薯X病毒CP基因3种结构对RNA沉默的诱导效果

Thus inducing efficiency can be checked by RAPD at the level of DNA molecular . Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression

以桃幼胚作为根癌农杆菌介导转化的受体，通过对GUS基因瞬时表达率的分析，研究此转化体系的最佳实验参数。

Using transient expression of GUS gene , the optimal conditions for Agrobacterium-mediated transformation of peach immature embryos was investigated .

随着愈伤组织培养时间的延长，GUS基因瞬时表达呈明显下降趋势，至第28天以后趋于稳定。

The expression efficiency of GUS gene reduced with the increase of callus culture time and remained stable after 28 d.

将该体系与PEG（聚乙二醇）介导的遗传转化法进行比较，瞬时表达率差异不显著。

Moreover , compared with transformation mediated by PEG ( Polyethylene glycol ), the transient expression efficiency of this system had no significant difference . 2 .

结论：LipofectAMINE介导pBK－Signal－EGF质粒转染角朊细胞可获得瞬时表达的EGF蛋白。

Conclusion : The instantaneous expression of EGF was obtained after gene transfer with the PBK-Signal-EGF plasmid mediated by LipofectAMINE .

HBX-GFP基因工程DN突变体瞬时表达及乙型肝炎病毒复制的实验研究

The inhibitory effects on hepatitis B virus replication by transient ex - pression of dominant negative mutants of hepatitis B virus X gene

将五个片段与荧光素酶cDNA拼接成荧光素酶瞬时表达载体并在小鼠乳腺中瞬时表达。

The five fragments and the luciferase cDNA were ligated together to get the luciferase temporary expression vector , which was temporarily expressed in the mammary gland of mice .