恶性疟原虫

用[α-~（32）P]&dATP标记恶性疟原虫DNA探针诊断恶性疟疾的研究

Studies on (α - ~ ( 32 ) p ) & datp labelled DNA probe of Plasmodium falciparum in diagnosis of falciparum malaria

目的构建恶性疟原虫海南株（FCC1HN）CSP3′末端基因的原核表达载体，并对CSP3′末端基因进行序列测定。

Objective To construct a prokaryotic expression vector of CSP3 ′ ending gene of Plasmodium falciparum Hainan isolate ( FCC1 / HN ), determine the sequence of CSP3 ′ ending gene .

用固相聚合酶链反应检测恶性疟原虫DNA特异片段

Detection of specific DNA fragment of Plasmodium falciparum by solid phase PCR

恶性疟原虫基因文库构建及特异DNA探针的应用研究

Construction of Plasmodium Genomic Library and Applications of the Specific DNA Probe

瑞香素对恶性疟原虫细胞色素C氧化酶及核糖核酸还原酶活性的影响

In Vitro Effect of Daphnetin on Cytochrome C Oxidase and Ribonucleotide Reductase of Plasmodium falciparum

应用四环素调控系统建立可调控抗恶性疟原虫DNA疫苗的初步研究

Preliminary study on regulable DNA vaccines against Plasmodium falciparum

恶性疟原虫质粒DNA探针在蚊媒监测中的应用研究

Applied Study of Plasmodium Falciparum Plasmid DNA Probe in Surveillance of Mosquito Vectors

重组恶性疟原虫DNA质粒免疫小鼠制备单克隆抗体

Preparation of monoclonal antibodies against malaria through immunization of mice with recombinant plasmid DNA

恶性疟原虫特异的质粒型DNA探针的制备

Preparation of plasmid DNA probe of Plasmodium falciparum

用生物素标记两种重组DNA探针检测红内期恶性疟原虫

Detection of Plasmodium falciparum using two kinds of recombinant DNA probes labelled with biotin

恶性疟原虫DNA疫苗在小鼠体内组织分布和安全性的初步研究

Distribution and safety of tissue in mice immunized with DNA vaccine of Plasmodium falciparum

恶性疟原虫抗原表位的亚单位疫苗与DNA疫苗的联合免疫

Combined vaccination of DNA vaccine and subunit vaccine of malaria antigen epitope by intranasal route

恶性疟原虫DNA疫苗在小鼠组织中的表达

The expression of DNA vaccine of Plasmodium falciparum in tissue of balb / c mice

恶性疟原虫FCC-1/HN株DNA疫苗在小鼠体内的免疫反应

Immune Responses on Mice Inoculated DNA Vaccines of FCC-1 / HN Strain of Plasmodium falciparum

目的建立恶性疟原虫荧光定量聚合酶链反应（PCR）检测方法，为快速、准确地定量检测恶性疟原虫奠定基础。

Objective To establish fluorogenic quantitative PCR method for detecting Plasmodium falciparum .

套式PCR检测滤纸干血滴中恶性疟原虫的研究

Detection of Plasmodium falciparum from dried blood spotted filter paper by nested PCR

恶性疟原虫保护性抗原复合基因DNA疫苗的构建及免疫学特征研究

Construction and Immunological Study of a DNA Vaccine from a Hybrid Gene of Protective Antigens of Plasmodium falciparum

用长距PCR法构建恶性疟原虫cDNA表达文库

Construction of Plasmodium falciparum cDNA Expression Library Using LD-PCR

恶性疟原虫云南株富含组氨酸蛋白II部分基因的克隆和序列分析

Cloning and sequence analysis of histidine rich protein ⅱ gene fragment of Plasmodium falciparum Yunnan Strain

恶性疟原虫重组质粒DNA接种诱导BALB/c小鼠的免疫应答

DNA vaccine of Plasmodium falciparum : immune response of balb / c mice induced by injection of the recombinant plasmid DNA

我们在常规PCR技术的基础上，建立了检测恶性疟原虫的荧光定量PCR方法。

Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate .

复式PCR检测恶性疟原虫与间日疟原虫的研究

Studies on detection of Plasmodium falciparum and Plasmodium vivax in blood samples by multiplex polymerase chain reaction

套式PCR用于海南省恶性疟原虫裂殖子表面蛋白1和2的分型研究

Genotyping of Merozoite Surface Protein 1 and 2 of Plasmodium falciparum Isolates from Hainan Province by Nested PCR

恶性疟原虫FCC（102）/JS株的建立

Establishment of fcc102 / js strain of Plasmodium falciparum

标记探针能与克隆DNA、恶性疟原虫Fcc1/HN株和云南株的PCR扩增产物杂交。

The labeled probe can hybridized with the PCR amplified products of cloned DNA , P.f Fcc1 / HN strain and Yunnan strain .

PCR扩增环子孢子蛋白基因3'端片段用于检测恶性疟原虫的初步研究

A preliminary study on detecting plasmodium falciparum based on PCR amplification of 3 ~ , - end fragment of CSP gene

恶性疟原虫反义HRPⅡ基因荧光真核表达重组质粒的构建

Construction of eukaryotic expression recombinant plasmid of antisense HRP ⅱ gene of Plasmodium falciparum

恶性疟原虫CSP基因重组蛋白及DNA免疫诱导小鼠体液免疫应答比较3.岭南黄鸡肌肉生长抑制素基因成熟肽的克隆及其真核表达载体的构建

Humoral immune responses in mice to the recombinant expressed protein and the nucleic acid vaccine of circumsporozoite protein gene of Plasmodium falciparum

与GenBank数据库中恶性疟原虫DNA比较，未发现与该cDNA相同的序列。

Comparison among cDNA of P falciparum from GenBank of EMBL showed that no sequence identical to this cDNA was found .

目的：应用四环素（tetracycline，Tet）可调控系统建立可调控的抗恶性疟原虫的DNA疫苗。

AIM : To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline ( Tet ) regulable system .