三氯丙酮

丙酮氯化反应液中1,1,3-三氯丙酮含量与比重的关系

Relation between content of 1,1,3-trichloroacetone and density of chlorination reaction mixture of acetone

棉花胚珠蛋白三氯乙酸丙酮法与酚抽提法的比较分析

Evaluations of Trichloroacetic Acid-acetone Method and Phenol Method for Protein Extraction from Cotton Ovule

用三氯乙酸/丙酮沉淀法提取纯化成曲中胞外酶系，利用SDS-PAGE电泳获得成曲胞外酶谱，进而对酶系差异进行分析。

TCA / acetone precipitation is used for extraction and purification , SDS-PAGE is used to obtain extracellular enzyme spectrum .

NLCS大脑神经节（cerebralganglion，CG）富含高丰度的脂类物质，并干扰了三氯醋酸-丙酮法沉淀CG蛋白质。

The cerebral ganglion ( CG ) in NLCS contains high abundant lipid compounds , indicating that the compounds interfere strongly the precipitation of CG proteins with trichloroacetic acid-acetone .

根据图谱质量比较，三氯乙酸/丙酮沉淀继以含硫脲的泡胀液复溶是优化的标本制备方法；

Deposition of trichloroacetic acid / acetone followed by resolubilization with rehydration buffer containing thiourea was the best sample preparation protocol ;

以新鲜鸡蛋清为材料，采用三氯醋酸-丙酮提取，冷丙酮沉淀，碳酸盐透析和DEAE-Cel-lulose离子交换层析分离出鸡卵清蛋白。

Ovalbumin was isolated from chicken egg by TCA-acetone solution extraction , cold acetone precipitation , sodium phosphate buffer dialysis and DEAE cellulose ion exchange chromatography .

本研究建立了适用于棉花叶片蛋白质组分析的蛋白提取方法和双向电泳体系。结果表明，三氯乙酸-丙酮沉淀法结合甲醇等有机溶剂洗涤纯化样品能获得纯度较高的棉花叶片蛋白样品。

The results showed that a high purity of cotton protein was extracted by TCA-acetone precipitation , and by cleaning the protein sample with solvent methanol or acetone .