枯草杆菌蛋白酶

用蛋白质工程的方法改良枯草杆菌蛋白酶E（SubtilisinE）的抗氧化性

Improvement of Antioxidation Property of Subtilisin E by Protein Engineering Method

属于枯草杆菌蛋白酶类的很多蛋白酶都有这样的现象。

Many proteins belonging to the subtilisin class have this phenomenon .

枯草杆菌蛋白酶E的156和165位突变

Site 156 and 165 Mutation of Subtilisin E

枯草杆菌蛋白酶E的蛋白质工程

Protein Engineering on Subtilisin E

采用十二烷基酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）方法，分析了几种商品蛋白酶（包括枯草杆菌蛋白酶、胰蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和细菌碱性蛋白酶）对大豆分离蛋白（SPI）的降解模式。

SPI hydrolysates induced by several commercial proteinases ( subtilisin protease , chymotrypsin , trypsin , papain , and Protex ) were analyzed by the SDS-PAGE .

文中还从蛋白质分子结构的角度，探讨了SPI的枯草杆菌蛋白酶水解多肽的聚集机理。

The mechanism of the aggregation of the peptides derived from SPI induced by subtilisin protease is also discussed in this paper in terms of the molecular structure of protein .

类枯草杆菌蛋白酶（Subtilisin-likeprotease，SLP）为丝氨酸蛋白酶家族成员，广泛存在于细菌、真菌和寄生虫等生物中。

The subtilisin-like protease ( SLP ) as members of the family of serine protease is widely existed in bacteria , fungi , protists and other organisms .

结果表明：在胰蛋白酶、木瓜蛋白酶、胃蛋白酶、枯草杆菌蛋白酶和复合风味蛋白酶5种酶中，胃蛋白酶为酶解鲢头制备ACE抑制肽的理想蛋白酶；

Isolation of the hydrolysates and the molecular weight of the peptide were measured by Sephadex G-15 . The results showed that there were five enzymes ( trypsase , papain , pepsin , subtilisin , flavourzyme ) that can hydrolyze silver carp head into hydrolysates .

研究了接种物和诱导物对球孢白僵菌总胞外蛋白酶及类枯草杆菌蛋白酶（Pr1）产生的影响。

Inocula and inducers with their effects on production of the total extracellular protease and subtilisin-like protease ( Pr 1 ) of Beauveria bassiana were studied .

应用UV光谱法研究在pH4.20的酸性溶液中，枯草杆菌蛋白酶（BSP）与茜素氨羧络合剂（ALC）的相互作用。

The interaction of bacillus subtilis proteinase ( BSP ) and Alizarin Complex ( ALC ) was investigated by spectrophotometric method in acidic solution ( pH 4.20 ) . When BSP was added into ALC , a red color was observed , which indicated the formation of BSP-ALC associated compound .

枯草杆菌蛋白酶与3,4-二羟基-2-蒽醌磺酸钠的作用机理

The Reaction Mechanism Between Bacillus Subtilis Proteinase and Sodium 3,4-Dihydro-2-anthraquinone sulfonate

枯草杆菌蛋白酶与茜素氨羧络合剂作用研究

Study on the Reaction of Proteinase from Bacillus Subtilis with Alizarin Complex

非水相体系中枯草杆菌蛋白酶-表面活性剂离子对的形成条件

Ion-pairing conditions between bacillus subtilis neutral proteinase and surfactant AOT in solvent

枯草杆菌蛋白酶热变性的热力学研究

Thermodynamic Study on the Thermal Denaturation of Subtilisin

吸附树脂载体固定枯草杆菌蛋白酶的研究

Study of Immobilized Subtilopeptidase on Adsorption Resins

因此，我们推测家蚕微孢子虫可能在入侵过程中会分泌类枯草杆菌蛋白酶，通过降解昆虫体壁帮助其入侵宿主细胞。

So we speculate that subtilisin-like protease in N. bombycis may participate in the process of invasion .

目的：在本实验室已克隆得到的灭蚊真菌卡地腐霉类枯草杆菌蛋白酶基因的部分序列的基础上，继续分离该基因的全基因组序列。

Objective : To obtain the full-long genomic sequence of the subtilisin-like protease ( Pr1 ) gene of Pythium carolinianum .

利用枯草杆菌蛋白酶对大豆分离蛋白进行酶法改性，改性后的大豆分离蛋白的功能特性得到很大的提高。

Soybean protein isolate ( SPI ) was enzymatically modified by subtilisin . Some functional properties of modified SPI were improved .

目的：分离和克隆灭蚊真菌卡地腐霉类枯草杆菌蛋白酶基因已知片段的3′端未知序列。

Objective : To explore the DNA sequence of the subtilisin-like protease ( Pr1 ) gene of Pythium carolinianum , a mosquito-killing fungus .

随着家蚕微孢子虫基因组测序的完成，通过序列比对发现在家蚕微孢子虫中也存在类枯草杆菌蛋白酶。

With the completion of genome sequence project of Nosema bombycis , it was found that subtilisin-like protease ( SLP ) also exist in this species .

两种方法水解获得的可溶性产物氨基酸及必需氨基酸组成含量接近，但胃蛋白酶、胰蛋白酶复合水解的可溶性产物具有更高的氨基酸分值，主要鲜味氨基酸含量略高于枯草杆菌蛋白酶水解产物，苦味氨基酸则相反。

The soluble products in the hydrolysate of the complex enzyme had more amino acid scores and higher delicious amino acids content than that of bacillus subtilisin , while the bitterness was on the contrary .

丝氨酸蛋白水解酶家族成员乙酰胆碱酯酶、α-糜蛋白酶、枯草杆菌蛋白酶的催化中心具有相似的结构特征，即由丝氨酸、组氨酸、天冬氨酸（谷氨酸）组成的催化三联体。

As the members of the serine protease family , acetylcholinesterase , α - chymotrypsin and subtilisin had the similar structural character in active sites . All of them had the catalytic triad composed of serine , histidine and asparagine ( or glutamic acid ) .

利用枯草杆菌碱性蛋白酶E基因的信号顺序构建分泌表达载体

Construction of Secretion Vectors Using Signal Sequence of Bacillus subtilis Alkaline Protease E Gene

枯草杆菌中性蛋白酶与Cu（Ⅱ）相互作用的EPR研究

Applications of EPR Spectroscopy to Study Interactions of Bacillus Subtilis Neutral Proteinase ( B.S.N.P. ) with Cu (ⅱ) Ions

结果表明，在吡啶溶剂中9种商业酶以枯草杆菌碱性蛋白酶催化Michael加成效果最好，并在50℃表现了最好的催化活性；

The effect of organic medium and enzyme resource on enzymatic tandem acylation / Michael addition and one-pot synthesis ; 8 enzymes have catalytic activity in 9 commercial enzyme , the catalytic activity of alkaline protease from Bacillus subtilis in anhydrous pyridine was the highest .

枯草杆菌碱性蛋白酶基因诱导表达载体的构建

Development of an inducible expression and secretion system for alkaline proteinase en Bacillus subtilis

枯草杆菌中性蛋白酶基因在大肠杆菌中的表达

Study on the Expression of Cloned Neutral Protease Gene of Bacillus subtilis in Escherichia coli

采用同时添加枯草杆菌中性蛋白酶与木瓜蛋白酶的改良酶解技术，对鲨鱼肉进行水解。

The shark meat was hydrolyzed with the enzyme hydrolytic technique in which subtilisin and papain were added simultaneously .

用枯草杆菌中性蛋白酶（AS1398酶）提取鱼蛋白酶水解物。

A fish protein hydrolysate was priqared from Anchovy Engraulis japonicus by using a neutral proteinase from Bacillus subtilis ( AS1398-ase ) .

Tween-80和鼠李糖脂对铜绿假单胞菌及枯草芽孢杆菌产蛋白酶的影响

Effect of Tween-80 and rhamnolipid on the production of protease from Pseudomonas aeruginosa and Bacillus subtilis