定向克隆

方法采用RT-PCR、定向克隆和DNA测序方法进行实验。

Methods : RT-PCR , directed cloning and DNA sequencing were utilized .

EB病毒胸苷激酶基因的定向克隆

Directional Cloning of Gene Coding Epstein-Barr Virus-specific Thymidine Kinase

结果酶切证实目的DNA定向克隆至载体上，测序分析结果与目的序列相同。

Results The target DNA was directly cloned to vector and the result was correct by sequence analysis .

绵羊肺腺瘤病毒NM株囊膜基因的定向克隆与融合表达

Cloning and fusion expression of env gene of sheep Jaagsiekte retrovirus NM strain

国人正常睾丸组织定向克隆表达cDNA文库的构建及意义

Construction and identification of directional expressing cDNA library from normal testes in Chinese males

目的研究精子发生相关基因的结构与功能，构建中国人正常睾丸组织定向克隆表达cDNA文库。

Objective To construct directional expressing cDNA library from normal testes in Chinese males for further studying the structure and functions of spermatogenesis related genes .

而定向克隆小麦HMWGS基因则为利用基因工程方法改良小麦品质提供新的基因资源，从而为优质小麦的发展起到积极的推动作用。

The cloned wheat HMW GS genes are new gene resource to improve wheat quality by gene engineering .

方法：采用PCR方法将FL和ESAT-6基因分别定向克隆入真核双表达载体pIRES。

Methods : FL and ESAT-6 genes were cloned into pIRES , a bicistronic vector , using polymerase chain reaction ( PCR ) .

经内切酶酶切和测序鉴定后，将由pMD-T-fbps内切酶切下的片段定向克隆于表达载体pET-32a（+），得到重组质粒pfbps。

The recombinant fragment of pMD-T-fbps was verified by restriction endonuclease analysis and sequencing .

材料和方法：本实验首先根据合成的引物，利用PCR反应产物，采用定向克隆的方法构建TrkC-cDNA真核表达质粒并进行DNA测序分析。

Methods : In accordance with the synthesized primer of TrkC , and by using TrkC gene produced through PCR , TrkC-cDNA expressed plasmid was constructed with directional cloning , and DNA sequencing analysis was performed .

将该基因定向克隆于真核表达载体中。

METHODS : The arresten gene was cloned to eukaryotic expression vector .

一个定向克隆的日本血吸虫基因表达库

A Directional Cloning Gene Expression Library of Schistosoma japonicum

醇溶蛋白的高效分离鉴定和编码基因的定向克隆，是研究麦醇溶蛋白及其与品质关系的可靠途径，并为基因工程改良小麦品质提供新的基因资源，从而推动小麦品质改良工作。

Separation and characterization of seed storage proteins and directed clone of their encoding genes are highly important for the study of gluten structure and the related relationship with quality , which can also provide alternative resources for improvement of wheat quality through gene engineering .

这里，我们使用定向RT-PCR克隆和DNA合成概述了我们最新的进展。

Here , we summarize our recent progress using directed RT-PCR cloning and DNA synthesis .

人β2肾上腺素受体cDNA的定向亚克隆

Directional Subcloning of β 2 adrenoceptor cDNA

大鼠α（1B）肾上腺素受体cDNA的定向亚克隆

Directional Subcloning of α 1B adrenoceptor cDNA in rats