亚克隆

新城疫病毒F蛋白七肽重复序列区的亚克隆、表达、纯化及结构分析

Subclone , Expression , Purification and Its Structure Analysis of Heptad Repeat Regions from the F Protein of Newcastle Disease Virus

结果构建的嵌合抗体基因在CHO细胞中的初始表达量为0.71μg/ml，亚克隆后表达量达0.95μg/ml。

Transfect CHO cells with the fusion gene and detect the expressed product . Results The primary expression level of the constructed chimeric antibody in CHO cells was 0.71 μ g / ml , and that after subclone was 0.95 μ g / ml.

长片段小麦细菌人工染色体DNA亚克隆文库的构建

Construction of Subclone Library for Wheat Megabase BAC DNA

大肠杆菌菌毛抗原K（99）基因的亚克隆及核苷酸序列测定

Subcloning and nucleotide sequencing of K_ ( 99 ) antigen gene of Escherichia coli

一株西尼罗病毒基因组cDNA亚克隆的构建与鉴定

Construction and identification of genomic cDNA subclones of West Nile virus

粉尘螨Ⅰ类变应原的cDNA克隆测序及亚克隆

Cloning , Sequencing and Subcloning of cDNA Coding for Group ⅰ Allergen of Dermatophagoides farinae

人β2肾上腺素受体cDNA的定向亚克隆

Directional Subcloning of β 2 adrenoceptor cDNA

MYO小卫星DNA探针及其片段亚克隆

MYO minisatellite DNA probe and sub & cloning of MYO fragment

人类DNA小卫星区域RNA探针制备及应用的研究（Ⅰ）&pGEM-4Z-MYO亚克隆的构建

Preparation of RNA probe of minisatellite region in human DNA and its practical use (ⅰ) - construction of sub-clone of pgem-4z-myo

大鼠α（1B）肾上腺素受体cDNA的定向亚克隆

Directional Subcloning of α 1B adrenoceptor cDNA in rats

泡桐基因文库的构建及actin基因同源顺序的亚克隆

Construction of Paulownia genome library and subcloning of the special DNA fragment homologous to actin gene

THP-1细胞CD14基因片段的克隆及亚克隆

Cloning and subcloning of CD14 gene fragment from THP 1 cells

变形链球菌表面蛋白PAc功能区基因重组质粒的亚克隆构建

Subcloning Construction of a Recombinant with Functional Regions of PAc Protein Encoding Gene of S. mutans

两个人组蛋白H1基因的亚克隆和它们启动子核苷酸顺序的比较

Two subclones of human histone H1 genes and comparison of their promoter sequences

参考人SRY基因Hmgbox的保守区序列，设计一对简并引物，用PCR扩增了扬子鳄Sox基因的Hmgbox，并对PCR产物进行了亚克隆和测序。

Bp fragments of Sox gene HMG box in Alligator sinensis were amplified by PCR , and then sub cloned and sequenced .

HO-1基因的亚克隆及策略

Strategies and Subcloning for Heme Oxygenase-1 Gene

登革2型病毒NGC株基因组亚克隆的构建及其鉴定

Construction and identification of genomic cDNA subclones of dengue 2 virus NGC strain

CD59基因的亚克隆

Subcloning of Human CD59 Gene

将cDNA部分片段亚克隆到原核表达载体（pQE-80L），转化宿主菌E。

Then a fragment of the cDNA was cloned to the expression vector pQE-80L . E.

PCR-SSCP分析急性淋巴细胞白血病TCR基因寡/亚克隆重排及临床意义

The study of oligoclonal / subclonal rearrangement of TCR gene in acute lymphoblastic leukemia with PCR-SSCP and it ′ s clinical significance

含抗霜霉病同源基因的亚克隆文库含有6200个克隆，覆盖BAC克隆的180倍左右。

The sub-clone library with downy mildew resistant genes consisted of 6 200 clones , covering 180-fold of the BAC clone in size .

对这一复制起点和来自自主状态的F质粒的复制起点进行了亚克隆，并作限制性内切酶酶切分析比较，没有发现两者在结构上有差异。

Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F ' plasmid and that from the autonomous F plasmid . No structural difference was found between them .

结果65例（80.2%）ALL病人存在TCRVγⅠJγ基因重排，8例（8.6%）病人存在寡/亚克隆重排；

Results 65 cases ( 80.2 % ) were found with TCR V γⅰ J γ gene rearrangement and 8 cases ( 8.6 % ) showed oligoclonal / subclonal rearrangement .

进一步亚克隆入表达载体pET-32a（+）。

Then it was sub - cloned into pET-32a ( + ) .

目的：研究人鼻咽癌亚克隆株F1、S1细胞的增殖能力、分化程度与放射敏感性的关系。

Objective : To study the proliferation and differentiation of human nasopharyngeal carcinoma subclones F1 and S1 with different radiosensitivity , and the relationship between them .

结论应用PCRSSCP方法可确定ALL的寡/亚克隆性，ALL病人寡/亚克隆检测有助于预测疗效和预后判断。

Conclusion Detection of TCR γ gene rearrangement by PCR SSCP can identify oligoclonality / subclonality in ALL patients and oligoclonal / subclonal rearrangement was potential in predicting prognosis of ALL .

GNA成熟蛋白基因亚克隆及其原核表达载体构建

Subcloning of GNA mature protein ( MGNA ) gene and its construction of expression vector in E.coli

结果建立分别稳定表达bcl-2基因、新霉素抗性基因（neo）的膀胱癌亚克隆细胞株BIU87/bcl-2、BIU87/neo。

Results The subclone bladder carcinoma cell lines , stably expressing bcl-2 and neo gene respectively , were successfully selected , named as BIU87 / bcl-2 and BIU87 / neo .

26例PCR扩增阳性病例经SSCP分析发现7例（26.9%）存在寡/亚克隆重排。

Among 26 cases with clonal TCRV γ I J γ〓 gene rearrangement , 7 cases ( 26 9 % ) was found with oligoclonal / subclonal rearrangement after PCR SSCP analysis .

弓形虫表面抗原P22编码基因片段的亚克隆与表达

Subcloning and expression of the gene encoding of the P22 surface antigen of Toxoplasma gondii