离心纯化

进一步大量制备其质粒DNA，并进行氧化绝密度梯度超离心纯化，以及电泳分析，电镜观察，并对质粒进行分离与鉴定，从而确定了每种质粒的分子量大小。

The molecular weight and size of these plasmids is determined .

用超速离心纯化的乙型脑炎病毒抗原，建立了间接ELISA方法检测乙脑IgG抗体。

With purified Japanese encephalitis ( JE ) virion , an indirect ELISA was developed to detect antibody of IgG class against JE .

大规模区带离心纯化Vero细胞乙脑疫苗

Large Scale Purification of Inactivated Japanese Encephalitis Vaccine from Vero Cells by Zonal Centrifugation

对差速离心纯化的汉坦病毒R84-1毒株进行了SDS-PAGE和免疫印迹试验。

SDS-PAGE and immunoblotting test of Hantavirus ( HTV ) R84-1 strain purified by differential centrifugation were carried out .

应用赤羽病病毒（AKV）OBE1株和牛标准阴阳性血清，以蔗糖密度梯度离心纯化细胞毒为包被抗原，在国内首次建立了检测AKV抗体的间接ELISA方法。

With the OBE-1 strain and the standard serum , the indirected ELISA has been developed for detecting antibody of Akabane virus in serum .

经PCR鉴定后，CsCl超离心纯化获得了滴度分别为1×1012和8×1011的BDNF和NT3重组腺病毒。

After two rounds of CsCl centrifugation , the human recombinant adenovirus Ad BDNF and Ad NT3 were obtained with titer of 1 × 10 12 and 8 × 10 11 pfu / ml , respectively .

结果：成功构建重组腺病毒Ad-siRNA-VEGF，293细胞包装1d后观察到绿色荧光蛋白表达，氯化铯梯度离心纯化获得约3.6×108efu/ml滴度的重组腺病毒；

Results : The recombinant adenovirus Ad-siRNA-VEGF was successfully constructed . One day after the linearized Ad-siRNA-VEGF in 293 cells being packed , green fluorescent protein ( GFP ) was observed . Ad-siRNA-VEGF with the titer of 3.6 × 108efu / ml was got by CsCl gradient purification .

建立了一种新的微孢子虫高速离心纯化方法。

A new method of microsporidia spore purification by high-speed centrifugation was established .

对比离心纯化与过滤纯化，并未发现两者之间的差异。

As for purification , there was no significant difference between filter and centrifugation methods .

方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。

Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation .

为探讨胰岛移植物的制备方法，采用胰管注射胶原酶、胰腺静止消化方法，分离成年大鼠胰岛，葡聚糖离心纯化。

Rat pancreatic islets were separated by intraductal collagenase injection and stationary digestion and were purified by dextran discontinuous density gradient .

扩增后收获病毒，氯化铯密度梯度离心纯化，空斑试验测滴度。

After acquisition , it was purified by chlorinated cesium ( CsCl ) density gradient ultracentrifugation . Its titer was determined by plaque assays .

方法采用肝胰管内灌注胶原酶消化分离胰岛及Ficoll400密度梯度离心纯化。

Methods The islets were isolated from rat pancreas by hepatopancreatic duct perfusing with collagenase and purified with Ficoll 400 discontinuous density gradient centrifugation .

应用胶原酶消化分离大鼠胰岛细胞，以Ficol密度梯度离心纯化，同时分离大鼠肝细胞。

The rat islets were isolated by using collagenase digestive method , purified by Ficoll density gradient centrifugation , and rat hepatocytes isolated simultaneously .

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

采用Percoll非连续密度梯度离心法纯化大鼠乳鼠心室肌细胞

Enrichment of cardiomyocytes from neonatal rat ventricular tissues by discontinuous percoll gradient centrifugation

结果经简化Percoll密度梯度离心分离纯化的滋养层细胞纯度高，明显优于淋巴细胞分离液的分离效果（P<0.001）；

Results More trophoblastic cells with higher purity were obtained by simplified Percoll gradient centrifugation than by lymphocytes separating solution ( P < 0.001 ) .

若将直接抽提的总DNA先经葡聚糖凝胶G200离心层析纯化，再用1%的琼脂糖凝胶电泳纯化，则能取得较好的纯化效果。

However , if the total directly extracted DNA first purify with Sephadex G-200 centrifuge filtration and then purify with 1 % Sepharose electrophoresis , a fairly good purification effect can be obtained .

结果：（1）Percoll密度梯度离心后纯化的间质细胞3βHSD染色阳性百分率可达85%。

Results : ( 1 ) The purified Leydig cell fraction after Percoll density-gradient centrifugation contained 85 % well-preserved 3 β - hydroxysteroid dehydrogenase ( 3 β - HSD );

以蔗糖密度梯度离心法纯化的病毒作为包被抗原，建立了检测鸭肝炎病毒（DHV）抗体的间接ELISA方法。

An indirect enzyme linked immunosorbent assay ( ELISA ) is developed for detection of antibodies against duck hepatitis virus ( DHV ) in ducks . The coating antigen was purified with sucrose density gradients centrifugation .

比较了4种从土壤中直接抽提的微生物总DNA的纯化方法，实验结果表明1%的琼脂糖凝胶电泳纯化方法及葡聚糖凝胶G200离心层析纯化方法均不能完全纯化从土壤中抽提的微生物总DNA。

Four methods to purify soil microbial total DNA extracted directly from soil were compared . The results showed that purification method with 1 % Sepharose electrophoresis and with Sephadex G-200 centrifuge filtration could not completely purify total DNA directly extracted from soil microbes .

方法：氯化铯密度梯度离心法纯化携带人野生型（rAd-SPKWT）及突变体（rAd-SPKDN）SPK的腺病毒，用噬斑分析和快速CPE法测定病毒感染滴度。

Methods : The adenovirus carrying the wild type SPK gene ( SPKWT ) and SPK mutant ( SPKDN ) were constructed respectively ; viral infection titer was determined with plaque assay and CPE method .

用蔗糖锑度密度离心初步纯化的酶与标准蛋白质作SDS聚丙烯酰胺凝胶电泳，得出脱氧苷酸羟甲基化酶的分子量为26000道尔顿。

This indicated that the enzyme involved was permeable through the cell wall . The enzyme was purified through sucrose density gradient centrifugation , and its molecular weight was proved to be 26.000 ( known 25,000 ) daltons by SDS polyacrylamide gel electrophoresis with known standard proteins as references .

采用正交试验对离心法纯化大豆异黄酮的条件进行了优化。

Orthogonal experiment was used to optimize the centrifugal purification conditions of soy isoflavones .

酒石酸钾-甘油密度梯度离心法纯化对虾肝胰腺细小病毒的方法学研究

Methodological study of the purification of the hepatopancreatic parvovirus of penaeid shrimp by a potassium tartrate glycerol density gradient centrifugation

方法采用改良的自动分离技术连续分离28例人胰岛，然后用连续性密度样度离心法纯化胰岛。

Methods 28 consecutive adult human pancreata were isolated with modified automated techniques . Islets were purified using continuous density gradient .

方法用胶原酶法分离、Ficoll400密度梯度离心法纯化胰岛。

Methods : Islets were isolated using digestion by ductal injection of collagenase , and purified by centrifugation of Ficoll density gradient .

病毒经差速离心法纯化后，四次免疫8周龄Balb/c雌性小鼠。

The 8-week-old female Balb / c mice were immunized with the allantoic fluid , which was purified and concentrated by differential centrifugation .

用饱和盐水漂浮法与用蔗糖密度梯度离心法纯化得到的卵囊具有相同的回收率和纯度；

No significant difference in the oocyst recovery , purity and infectivity was found between methods using saturated saline floatation and sucrose density gradient centrifugation .

为进一步提高大豆异黄酮的含量，满足其精细化的需要，采用正交试验对离心法纯化大豆异黄酮的条件进行了优化。

In order to increase the content of soy isoflavones and meet the requirement for further fined-processing , the orthogonal experiments were used to optimize the purification conditions of soy isoflavones in centrifugal process .