酶标记

胶体金技术是继同位素、荧光素、酶标记技术之后的一种新型标记技术，它具有快速、敏感、便捷、不需特殊设备、结果判断直观等优点。

Colloidal gold technique is a novel labeling technique after isotope , fluorescin and enzyme labeling , which has the merits such as easy , rapid , sensitive and ready-to-read .

一种敏感、快速、特异的抗双股DNA测定法&短膜虫酶标记抗体技术

A Sensitive , Rapid and Specific Assay for Double Stranded DNA Antibodies

以辣根过氧化物酶标记的沙门氏菌O9单抗3-47-0与包被的鸡白痢沙门氏菌脂多糖抗原建立了一种抗体阻断ELISA以检测鸡白痢和鸡伤寒沙门氏菌感染。

A blocking ELISA was developed for diagnosing pullorum disease and fowl typhoid .

用辣根过氧化物酶标记IL-2单克隆抗体。

The monoclonal antibody against IL-2 was labelled with horseradish peroxidase .

ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。

ELISA is based on solid-phase antigen or antibody and antigen or antibody-based enzyme marker .

方法使用不同的酶标记物，观察ELISA法检测培养上清液中单链抗体的相对灵敏度。

Methods Relative sensitivity of various conjugates in ELISA for the detection of ScFv was assessed .

HBsAg的酶标记及其在检测抗&HBs的ELISA系统中的应用

Enzyme-labelling of HBsAg and Application of the Conjugate to the ELISA System for Detecting Anti-HBs

抗宫颈癌单克隆抗体AU（14－1）的辣根过氧化物酶标记

Conjugation of Monoclonal Antibody Against Uterine Cervical Cancer AU_ ( 14-1 ) with Horseradish Peroxidase

酶标记抗人IgM单克隆抗体的制备及其初步鉴定与应用

Preparation of Enzyme-Labelled Anti-Human IgM McAbs and Their Preliminary Characterization and Application

本实验应用单克隆抗人精子抗体和酶标记羊抗人精子抗体，采用ELISA方法确定精子抗原成份的存在。

The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm .

应用DNA聚合酶和末端脱氧核糖核酸转移酶标记DNA链断端的核酶（TUNEL）法检测以上贲门组织中的凋亡细胞，计算凋亡指数（AI）。

TUNEL was used to detect the apoptosis cells in the above-mentioned cardiac tissue , and to calculate angiogenesis index .

无HindⅢ位点新霉素磷酸转移酶标记基因的建立

Construction of a neomycin phosphotransferase gene without hind ⅲ site

传染性非典型肺炎冠状病毒特异性IgG抗体检查应用酶标记免疫吸附测定法进行检测。

The severe acute respiratory syndrome coronary virus specific immunoglobulin G antibody was detected with enzyme-labelling immunosorbent assay .

目前已经有不少研究者在棉花种内、种间构建了遗传连锁图谱，使用的标记包括各种形态学标记、细胞学标记、同功酶标记、DNA标记。

At present , many researchers have constructed some genetic linkage maps for cotton , by means of morphological markers , cytogenetical markers , iso-enzyme markers and molecular markers .

方法：以特定引物和16mer的随机引物作为延伸引物，利用Taq酶标记DNA探针。

Methods : Based on PCR principle , DNA was labeled using specific and random primers . The E.

方法用SPA法在载体上固定系列浓度酶标记抗体，绘制抗体浓度-吸光度曲线，寻找SPA与抗体的最佳结合条件。

Methods Used SPA method to immobilize the enzyme conjugated antibody of different concentrations ! and drew the antibody-OD line .

用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志

Detection of CEA Marker of Colon-Rectal Carcinoma under Light and Electron Microscopes Using Horseradish Peroxidase Labeled Anti-CEA Fab Antibody

使用辣根过氧化物酶标记葡萄球菌蛋白A（HRPPA）对大鼠腑内β内啡肽（β-End）免疫反应细胞进行定位。

β - endorphin-immunoreactive cells in the rat brain were localized using the horseradish peroxidase conjugated staphylococcal protein A ( HRP-PA ) .

然后温育1h洗涤除去非结合（酶标记）试剂。

After an incubation time of 1 hour , the non-bound ( enzyme labelled ) reagents were removed in a washing step .

我们认为酶标记抗原直接ELISA法为HFRS的早期诊断提供了更为特异、简便快速的新方法。

We think that direct ELISA that uses HRP labeled antigen provides a more specific , simpler and faster method for early di agnosis of HFRS .

APAAP桥联酶标记技术结合免疫金银染色进行免疫组化双重标记也取得了满意的标记效果。

The APAAP technique could be used for doube immunohistochemical labeling combined with immuno-gold silver staining .

本文采用辣根过氧化物酶标记伴刀豆球蛋白A（conA-HRP）法来研究人和犬、大小鼠消化管各段conA受体的分布。

The distribution of Con A receptor in gastrointestinal tract of human , rat , mouse and dog was investigated using horseradish peroxidase labeled Con A staining method .

游离氯霉素与氯霉素辣根过氧化物酶标记物（HRP）在同一系统中竞争数量有限的氯霉素抗体结合位点，氯霉素酶标记物与氯霉素抗体结合的数量反比于游离氯霉素的数量。

Free CAP and CAP-HRP are competitive to CAP antibody binding site in the same system . CAP-HRP binding amount is in inverse proportion to free CAP .

在胰腺癌标本中用TdT酶标记的DNA3/-OH末端分析连续切片表明，大多数癌细胞未发生细胞凋亡，在40例胰腺癌中仅有3例发现约10%的癌细胞凋亡。

Apoptotic signal was present in approximately 10 % of the tumor cells in only 3 of 40 cancer samples .

取乙型肝炎患者Delta抗体（抗HD）阳性血清，经硫酸铵盐析和DE52柱层析，得到纯化的抗HD抗体，用辣根过氧化物酶标记。

The antibody to hepatitis delta virus ( Anti-HD ) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase ( HRP ) .

GL-7-ACA酰化酶标记复合物的结晶过程研究；

Study on the crystallization process of labeled GL-7-ACA acylase complex ;

以辣根过氧化物酶标记抗体，建立拟除虫菊酯的直接竞争抑制ELISA（DC-ELISA）检测方法，进一步优化分析条件并建立了一种适用于蔬菜样品中菊酯残留快速检测的规程（protocol）。

A direct competitive ELISA ( DC-ELISA ) was established by using the highly generic antibody against PBA , which was labeled with Horseradish Peroxidase ( HRP ) .

在实验中采用EHF病毒抗原与酶标记物混合后一次加入，而不是分别依次加入的方式，使操作步骤简化，实验时间缩短，又适当提高了试验敏感性。

In order to simplify the procedure , shorter test time and promote the sensitivity , the EHF virus antigen and HRP-McAb were mixed up then added simultaneously .

采用流式细胞仪（FCM）检测肝、肌细胞Ca2+浓度及DNA倍体分析，末端转移酶标记（TUNEL）技术检测细胞凋亡。

The flow cytometry ( FCM ) was used to examine the Ca 2 + concentration in myocytes and hepatocytes and the DNA double analysis was conducted . The apoptosis was examined by using the TUNEL technique .

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .