酶切位点

结果表明，牛乳铁蛋白启动子区在HinfⅠ酶切位点存在多态性，其酶切位点由2种共显性等位基因控制。

The results show that bovine lactoferrin in the promoter region of Hinf ⅰ restriction site polymorphism exists , the restriction site by the two kinds of control of codominant alleles .

通过PCR方法成功扩增了材料基因组中的EPSPS基因，并添加了适当的酶切位点。

The EPSPS gene was amplified by PCR and added the suitable restriction site .

对重组子测序结果表明，实现了DNA小片段和酶切位点的定向引入。

The results showed that the sites and low molecular weight DNA were successfully introduced .

中国人内源性高甘油三酯血症与载脂蛋白CⅢ基因SstⅠ酶切位点多态性关联的研究

Apolipoprotein c ⅲ gene sst ⅰ polymorphism in relation to endogenous hypertriglyceridemia in Chinese population

利用PCR方法，从含有3G1scFv基因的重组质粒中扩增出抗体基因，并使基因两端携带合适的限制性酶切位点。

3Gl scFv gene fragment was amplified and proper restriction enzyme sites were added at each end by PCR .

汉坦病毒基因组G1区部分酶切位点的研究

Study on the Endonuclease Site of G1 Region of Hantavirus Genome

2型糖尿病与脂蛋白脂酶基因HindⅢ酶切位点的多态性

Polymorphism of lipoprotein lipase gene Hind ⅲ associated with type 2 diabetes

pBⅠ121载体酶切位点添加及拟南芥Na~+/H~+逆向转运蛋白基因表达载体的构建

Modification of pB ⅰ 121 Vector and Expression Vector Construction Na ~ + / H ~ + Antiporter of Arabidopsis thaliana

中国钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类研究

Identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene

猪生长激素基因ApaⅠ酶切位点多态性分析

Analysis of Apa ⅰ polymorphisms in porcine growth hormone gene

根据烟草中柠檬酸合成酶（citratesynthase，cs）基因的序列设计两个上下游引物，并在上下游引物中分别加入NcoⅠ和XbaⅠ酶切位点。

Primers were designed according to the nucleotide sequence of citrate synthase ( cs ) gene .

应用创造酶切位点PCR-RFLP检测乙醇脱氢酶2基因多态性

Application of created restriction site PCR-RFLP to identify alcohol dehydrogenase 2 gene polymorphism

唐山地区原发性高血压与Y染色体非重组区HindⅢ酶切位点多态性的研究

Study on polymorphic Hind ⅲ restriction site of the Y chromosome and essential hypertension in Tangshan district

鼠疫耶尔森氏菌16S-23SRRNA基因间区的酶切位点分析

Analysis on the Physical Pattern of 16S-23S rRNA Spacer Region of Y.pestis

目的：为了研究日本人群β-链基因AvaⅡ酶切位点的遗传多态性以及该位点等位基因频率的分布。

Objective : In order to explain the distribution of polymorphism gene .

结论Y染色体HindⅢ酶切位点多态性与老年男性原发性高血压有关，其可能是老年男性原发性高血压的一个遗传标志。

Conclusion Polymorphic Hind ⅲ restricted site of the Y chromosome is possibly associated with gerontal male essential hypertension in China .

方法82例汉族女性雄激素过多症患者及14名健康女性进行ACTH兴奋试验，并应用PCR扩增产生限制性酶切位点方法检测已知的9个21-OHD常见突变位点。

Methods Eighty-two androgen excess cases and 14 healthy women underwent ACTH stimulating test during the follicular phase .

牛疱疹病毒Ⅳ型（DN-599株）DNA的限制性酶切位点图及重复序列分析

Physical mapping of bovine herpesvirus type 4 ( dn-599 strain ) DNA and analysis of its repetitive sequences

汉族人和法国人Ⅰ型胶原α链Spl结合序列MscⅠ酶切位点基因多态性的比较

Difference in Spl binding site polymorphism at collagen type ⅰα 1 gene between healthy adults of Han nationality and French nationality

根据序列的酶切位点分析，选择DraⅠ和HindⅢ内切酶，对配对的食管癌组织和非癌食管组织DNA酶切，Southern印迹杂交分析。

Restriction endonuclease Dra ⅰ and Hind ⅲ were used to digest genomic DNA in the pair matched cancerous and non cancerous esophageal tissues for Southern blot analysis .

方法：用PCR法从绿脓杆菌基因组DNA中克隆PEAⅡ＋Ⅲ区基因，引入Kozak序列、终止信号和酶切位点，测序。

Methods : Polymerase Chain Reaction was employed to clone PEA domain ⅱ + ⅲ structural gene , introducing Kozak sequence , termination code and restriction sites .

结果显示：中国汉族人群LDL受体基因中存在着TaqⅠ酶切位点多态性；

The results showed that the Taq ⅰ polymorphism is associated with the LDL receptor gene in Chinese of Han nationality ;

基因启动子CpG岛HapⅡ酶切位点的甲基化与Kilon基因低表达无相关性。

The promoter methylations at Hap ⅱ loci is not correlated to the low expression of Kilon gene .

目的：探讨高脂血症患者脂蛋白酯酶基因内含子8HindⅢ酶切位点多态性与HDL亚类组成的变化关系。

AIM : To investigate lipoprotein lipase gene Hind ⅲ polymorphism and its relationship with serum lipids and apolipoprotein , serum HDL subclasses in patients with hyperlipoidemia .

利用生物软件进行分析，获得cDNA序列碱基组成、密码子使用频率和酶切位点等详细数据以及蛋白质理论分子量、等电点和氨基酸组成等情况。

With biological software analysis , obtained cDNA sequence base composition , codon usage and restriction site data , as well as detailed theoretical protein molecular weight , isoelectric point and amino acid composition .

2型糖尿病及胰岛素抵抗与脂蛋白脂酶基因HindⅢ和PvuⅡ酶切位点多态性分析的研究

DNA Polymorphism of Hind ⅲ and Pvu ⅱ Sites in the Lipoprotein Lipase Gene of Patients with Type 2 Diabetes Mellitus and Insulin Resistance

山东省汉族人群维生素D受体基因FokⅠ酶切位点多态性与胃癌易感性的相关性研究

Correlation Research between Gene Polymorphism of Vitamin D Receptor Fok ⅰ Restriction Endonuclease and Susceptivity of Gastric Cancer in Han People of Shandong Province

方法采用基因定点突变方法，在新城疫病毒（NDV）F与人副流感病毒（hPIV）F基因上创造相同的酶切位点。

Methods Site-directed mutagenesis was used to create the same enzyme sites on the F gene of Newcastle disease virus ( NDV ) and human parainfluenza virus ( hPIV ) .

酶切位点多态性研究结果表明，在pGH基因全序列中存在着丰富的酶切位点多态性。

The results of restriction enzyme sites indicated that there were abundant sequence polymorphisms in the full sequence of pGH gene .

方法：根据GenBank登记的K9基因核苷酸序列设计一对PCR引物，其5′端分别引入HindⅢ和BamHⅠ酶切位点。

Methods : A pair of PCR primers for K9 gene with Hind ⅲ and BamH ⅰ restriction enzyme cut sites were designed according to the sequence registered in GenBank .