酵母双杂交系统

人Era不同结构域在酵母双杂交系统中的表达及对报告基因激活作用的检测

Reporter Genes Assay of the Different Domain of Human Era in Yeast Two-hybrid System

利用酵母双杂交系统筛选与G蛋白及CaM相互作用的新蛋白

Screening of Novel Proteins That Interact with G Protein or CaM Using Yeast Two-hybrid System

酵母双杂交系统筛选与M1相互作用的蛋白质

Screening Proteins to Interact with M1 by Yeast Two-hybrid System

酵母双杂交系统用于TPO分子间相互作用的研究

Study on interactions between TPO molecules tested with yeast two-hybrid system

目的构建大鼠糖皮质激素受体（GR）各个结构域的酵母双杂交系统，以研究不同结构域在糖皮质激素非基因组效应的作用。

Objective : To research the action of glucocorticosteroid receptor domains in the glucocorticosteroid non-genomic effect .

本文利用酵母双杂交系统分别筛选了与这两种蛋白相互作用的蛋白质，以进一步研究G蛋白和CaM信号转导途径中的其它上下游组分。

In order to study the upstream and downstream components in G protein and calmodulin signal pathways , a yeast two-hybrid system was used to screen interaction partners of these two proteins .

采用酵母双杂交系统对P6进行研究。

Yeast two-hybrid system were adopted on the study of P6 .

利用酵母双杂交系统研究人血管内皮生长因子受体Flt-1的配体结合域

Study on Ligand Binding Domains of Human Vascular Endothelial Growth Factor Receptor Flt-1 by Yeast Two-Hybrid System

Tec家族蛋白酪氨酸激酶ItkPH结构域的克隆鉴定及其在酵母双杂交系统中自激活作用的检测

Cloning and Identification of Tec Family Protein Tyrosin Kinase Itk PH Domain and Detection of its Self activation in the Yeast Two hybrid System

应用酵母双杂交系统筛选hTNFα特异性结合肽

Screening for Specific hTNF α Binding Peptide with Yeast Two-hybrid System

BI-1作为酵母双杂交系统诱饵的载体构建

Construction of DNA-BD Vector with BI-1 for Yeast Two-hybrid Analysis

方法：构建含有APP羧基端106个氨基酸的融合表达质粒，用酵母双杂交系统筛选人胎脑cDNA文库。

Methods : A fusing expressed plasmid , which contains the 106 amino acids at the carboxyl terminal of APP , was constructed . Screening against human embryo brain cDNA library by two hybrid system was performed .

本研究在蛋白筛选的基础上，利用酵母双杂交系统对病毒蛋白与寄主蛋白之间的互作做进一步的验证，并针对RSV编码的功能蛋白NS2，对其互作情况进行了研究。

Based on library screening , the yeast two hybrid system was employed to further confirm the interaction of viral protein and host protein .

酵母双杂交系统（YTHS）是Fields等建立的一种灵敏的检测酵母细胞蛋白-蛋白互作的试验方法。

Yeast two hybrid system ( YTHS ), established by Fields and his colleague , is an sensitive technology to identify the protein-protein interaction in yeast cell .

为研究tTG在细胞凋亡过程中发挥作用的机制，利用Gal4酵母双杂交系统筛选了HeLa细胞中与tTG相互作用的蛋白质，获得了17个阳性酵母克隆。

To investigate the mechanism of tTG in cell apoptosis , yeast two hybrid system was used to screen HeLa cDNA library .

目的应用酵母双杂交系统筛选人血管内皮生长因子受体Flt-1胞外最小配体结合域。

Aim To screen the minimal extracellular ligand-binding domain of vascular endothelial growth factor ( VEGF ) receptor Flt-1 using yeast two-hybrid system .

用酵母双杂交系统筛选CENP-E相互作用蛋白

Screening Proteins that Interact with Centromere-associated Protein E via Yeast Two-hybrid System

利用酵母双杂交系统鉴定MIF与CD74胞外片段间的相互作用

Identification of the interactions between the truncated fragments of macrophage migration inhibitory factor and CD74 using a yeast two-hybrid system

为研究其在白血病细胞中的作用机制，本研究拟利用酵母双杂交系统从自行构建的HL鄄60细胞cDNA文库中筛选出与蛋白激酶CK2α'亚基相互作用的蛋白。

In order to investigate the mechanism of its effect on hematopoietic cells , we used yeast two-hybridization screening the proteins interacting with protein kinase CK2 α′ subunit from HL-60 cells cDNA library .

以大T抗原和p53蛋白的相互作用为阳性对照，建立酵母双杂交系统，并就酵母转化实验中如何提高转化效率、酵母菌株培养条件的掌握、变异、保存问题进行了研究。

Interaction between the SV40 large T-antigen and the p53 protein as a positive control was established for the yeast two-hybrid system . Some interested questions were probed in the yeast transformation . It contains the transformation efficiency and the culturing yeast and handling yeast .

目的构建DLX4酵母双杂交系统并鉴定其自激活作用。

Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation .

结论柯萨奇病毒B3型VP1蛋白可作为酵母双杂交系统的诱饵蛋白，为筛选与其相互作用的宿主细胞蛋白提供了基础。

CVB3 VP1 protein can be used as a bait protein for the yeast two-hybrid system , which provided a basis for screening proteins of host cells that interact with VP1 protein .

介绍了基因组步移、凝胶阻滞、酵母双杂交系统、cDNA微阵列等基因转录调控的常用研究方法，并对藻类基因转录调控的最新研究进展进行了评述。

In this paper , the widely used techniques for the study of transcriptional control , such as genome walking , electrophoresis mobility shift assay , yeast two-hybrid system , cDNA microarray and so on are introduced and the recent progress in the transcriptional control of algae gene is discussed .

本研究采用酵母双杂交系统探寻与神经病靶标酯酶（NTE）调控结构域相互作用的蛋白因子，揭示与NTE信号转导相关的可能机制。

To explore protein factors that may interact with regulatory domain of neuropathy target esterase ( NTE ) in signal transduction pathways , the bait plasmid of NTE - regulatory domain cDNA was constructed and screened in human fetal brain cDNA library by the method of yeast two-hybrid .

研究蛋白质相互作用的新系统&酵母双杂交系统

The Yeast Two-Hybrid System : a New Way for Studying Protein-Protein Interactions

方法构建缺失突变体，利用酵母双杂交系统进行测试。

Methods Four deletion mutants were constructed and cloned using yeast-two-hybridization system .

以酵母双杂交系统筛选与N-乙酰氨基葡萄糖基转移酶Ⅲ相互作用的蛋白质

Screen for proteins that can interact with N-acetylglucosaminyltransferase ⅲ with yeast two-hybrid system

酵母双杂交系统的改进和发展

The Improvement and Progress of Yeast Two-hybrid System

分裂泛素化酵母双杂交系统研究光合膜蛋白相互作用

Studying of The Thylakoid Membrane Protein Interactions Through The Split-ubiquitin Yeast Two Hybrid System

酵母双杂交系统及发展

The yeast two-hybrid system and its development