读框

该因子没有能够编码类似TNP1/TNPA的DNA结合蛋白的开读框。

No open reading frame encoding TNP1 / TNPA-like DNA binding protein were found in this element .

目的：通过扩增角鲨烯环氧化酶基因的开放读框，进行PCR来鉴定白念珠菌。

Objectives : To identify Candida albicans by PCR amplification of the open reading frame coding squalene epoxidase .

coliTAP（106）后，获得pYX（40）对重组子的酶切图谱、原位杂交及DNA序列的分析表明，插入基因的方向、读框正确。

Coli TAP 106 . Analysis of restriction enzyme mapping , hybridizing and DNA sequencing shows that the gene direction and reading frame are correct .

GAM分子具有一个588个核苷酸的开放读框，编码196个氨基酸。

GAM cDNA showed an open reading frame of 588 nucleotides coding for an 196 amino acids protein .

经过序列测定，证明12个gsp基因读框正确，保障了gsp基因在酵母体系中正确的表达。

The open reading-frames of 12 gsp genes were verified by sequening , which ensure that gsp genes can be expressed rightly in yeast system .

以总RNA为模板，根据人IL-10在GenBank中的全长开放读框设计的特异性引物进行反转录，RT-PCR进行IL-10基因筛选。PCR产物经酶切分析和DNA序列测定后；

Then , RT-PCR was carried out for IL-10 gene screening using total RNA as a template , specific primers which derived from GenBank data of hIL-10 as a primer for reverse transcription and PCR primers .

结果NYDCh和NYDTr基因开读框大小分别为816bp和786bp，预计可分别编码272个和262个氨基酸。

Results The sizes of NYD-Ch and NYD-Tr genes were 816 bp and 786 bp , encoding 272 and 262 amino acids , respectively .

在B19病毒基因组内有两个大的开放读框，编码非结构蛋白（NS）和两个衣壳蛋白（VP1和VP2）。

There are two big open reading frames in the genome , which encode the nonstructure protein ( NS ) and the two capsid proteins ( VP1 and VP2 ) .

HCV为一单股正链RNA病毒，具有很大的开放读框（ORF），其基因组由结构蛋白基因及非结构蛋白基因组成，分别编码结构蛋白、外膜蛋白及相应的非结构蛋白。

HCV is a positive strand RNA virus which contains a large open reading frame ( ORF ) . HCV genome is consist of structural gene and non-structural gene that encode structural protein , envelop protein and non-structural protein respectively .

内部核糖体进入位点（IRES）序列来源于脑心肌炎病毒，它可翻译一条mRNA上的两个开放读框，由其连接的两个基因的表达率相同。

The internal ribosome entry site ( IRES ) sequence was derived from encephalomyocarditis virus . It allows to translate two open reading frames at one mRNA , so two genes conjoined by IRES have the same expression rate .

序列分析和限制酶切图谱表明所克隆的鲤鱼生长激素基因的开放读框含有630bp，编码210个氨基酸，其中含有22个氨基酸的信号肽和188个氨基酸的成熟GH序列。

The result of sequence analysis and restriction map shows that an open reading frame of carp growth hormone gene contains 630 base pairs which code for a polypeptide of 210 amino acids including 22 amino acids of the signal peptide and 188 amino acids of the mature growth hormone .

其中人era-cDNA基因全长2.2kb，含长度为1038bp的开放读框，编码346个氨基酸的蛋白质。

The full length of human era-cDNA gene is 2.2 kb and contains an open reading frame of 1038 bp in length that encodes a protein of 346 amono acids .

白念珠菌角鲨烯环氧化酶基因开放读框的克隆及其在大肠杆菌中的表达

Molecular cloning and expression of squalene epoxidase from Candida albicans in E.coli

结果成功克隆了人胃动素受体基因表达片段，测序证实读框正确。

Results Human motilin receptor was successfully cloned with correct open reading fragment .

读框分析表明，该序列编码含200个氨基酸的蛋白质。

On the Frame Matrix it can interpret a protein containing about 200 amino acid residuals .

序列分析结果表明：该2432bp的序列含2个开放读框，即鸡蛋花花叶病毒移动蛋白基因序列和外壳蛋白基因序列。

It showed that the fragment of 2432 bp had two open reading frames ( ORF ) .

某些杆状病毒和痘苗病毒的基因组中具有编码酪氨酸蛋白磷酸酯酶的开读框，与感染性和致病性有关。

Some baculoviruses and orthopoxviruses have open reading frames encoding protein tyrosine phosphatases ( PTPs ) which are related with the infectivity and virulence of the viruses .

结果重组的融合蛋白表达载体经酶切、PCR和DNA序列测定证明构建正确，读码框无移位。

Results The recombinant fusion protein expression vector was verified correctly by enzyme digestion , PCR and sequence analysis .

第二种剪切方式得到一个短的cDNA，读码框只编码38个氨基酸。

In the second splicing pattern , the cDNA has a short open reading frame which encodes a protein of 38 AA .

开放读码框末端有由TAA组成的终止密码子。

The termination codon was composed of TAA .

之后，这些小的反义RNA可能会在RNA拼接中发挥作用，如在选择压力下修复基因组中一个区域，抑或在丧失或损坏一个模块后恢复一个读码框。

Such small antisense RNAs may later have gained a role in RNA editing , possibly under selective pressure to repair a region or restore a reading frame after loss or erosion of a module .

方法用巢式聚合酶链反应（nestPCR）技术对淮阴地区220名专业献血者进行血清中输血传播病毒（TTV）的检测，并在PUC18中对其开放读码框2（ORF2）基因进行了克隆。

Methods TTV DNA in sera from 220 blood donors in Huaiyin was detected by nest polymerase chain reaction ( nest ? PCR ) .

PCR产物经过双酶切后按照正确的读码框顺序克隆到毕赤酵母表达载体pPICZαA中；

PCR products was inserted into the plasmid pPICZ α A after digested by the Xho ⅰ and Xba ⅰ restriction enzymes according to the correct reading code frame ;

预测和注释了45个开放读码框（ORF），分析了DNA复制基因、衣壳蛋白和DNA包装基因、侵染相关基因。

Putative forty-five open reading frames ( ORF ) were annotated , homology analysis were used to assign possible functions for the genes , including , DNA replication , synthesis of capsid protein , DNA packaged genes , and infection-associated genes .

经测序分析，最终在8个棉花品种中得到74条具有完整开放读码框的棉花RGAs。

The clones are sequenced and 74 RGAs with complete open reading frames ( ORF ) from eight varieties of cotton are finally obtained .

基因盒往往由一个编码抗生素抗性的开放读码框（ORF）和一个整合位点attC组成。

One gene cassette usually contains a open reading frame encoding antibiotic resistance and a specific recombination site called attC ( or 59-base element ) .

且基因起始端Kozak序列完全，基因终止子已经突变、读码框正确。

In the gene start area , there was an intact Kozak sequence . Terminator of gene was mutated and the reading frame was correctly identified .

通过PCR扩增和PCR产物直接克隆，将黄杆菌质粒上的一段长达1137bp的opd基因进行扩增及克隆，然后按正确的读码框亚克隆于表达载体pET28b（+）上，转化宿主菌E。

The 1137 bp DNA fragment encoding organophosphorus hydrolase was amplified with PCR , then directly cloned into pGEM-T vector . Plasmid pET-opd was constructed by subcloning the opd gene into plasmid pET28b ( + ) in correct reading frame .

目的：构建马动脉炎病毒读码框ORF5、ORF6、ORF7主要结构蛋白基因全长片段的克隆载体及汉逊酵母穿梭载体，并获得重组工程菌，为下一步酵母表达重组目的蛋白奠定基础。

Objective : To construction the cloning and yeast expression vectors containing ORF5 、 ORF6 、 ORF7 structural protein gene of equine arteritis virus and lay foundation for expressing these recombinant protein .

利用高保真酶扩增特性调整基因读码框的方法

A Convenient Method for Condon Adjustment by Using High-fidelity DNA Polymerase Amplification Property