羊痘

并能与羊痘病毒、口蹄疫病毒鉴别；

It can distinguish from sheep pox virus and foot-and-mouth virus ;

目前，羊痘已遍布世界大部分的国家和地区，以非洲、亚洲最为严重，我国多地也均有发生，有的地区呈暴发流行，并有上升趋势。

At present , sheep pox and goat pox has all over the world , especially in Africa and Asia , also they are widely epidemic in our country , some areas were outbreaks and have a rising tendency .

利用ELISA方法检测3个纯化重组表达蛋白活性，结果证明3种表达蛋白均与羊痘标准阳性血清有反应性。

The results of ELISA demonstrated that the three expressed proteins shared reaction with standard positive serum of capripox .

羊痘病毒P32蛋白基因的克隆及其杆状病毒表达载体的构建

Cloning of the gene of capripoxvirus major antigen P32 and construction of its baculovirus expression vector

羊痘病毒P32蛋白的表达

Expression of Capripoxvirus P32 Protein

本试验为羊痘病快速有效诊断方法的建立和CPV的分子生物学特性的研究奠定了基础。

This assay provided basis for the establishment of rapid and available goat and sheep pox diagnosis methods and for the study of CPV molecular properties .

羊痘病毒P32蛋白是位于羊痘病毒膜表面的主要结构蛋白，含有中和抗原表位；

P32 protein is one of the virus structure proteins . It stands out on the virus envelope surface and contains neutralized antigen epitope .

主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；

The following work were done in this study : ( 1 ) TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK ;

采用硫酸铵盐析、SephadexG-200柱层析的方法纯化山羊痘病毒，以病毒抗原致敏绵羊红细胞建立了山羊痘正向间接血凝试验，并进行了最佳试验条件的摸索。

The goat pox virus ( GTPV ) has been purified through ammonium sulfate sedimentation and Sephadex G-200 chromatography . The sheep erythrocyte was sensitized with purified virus and the indirect hemagglutination test ( IHA ) was developed .

从羊痘病毒基因组中寻找并扩增羊白细胞介素-18结合蛋白（IL-18BP）基因。

Some virus genome of vaccinia subgenus in poxvirus encoded IL-18BP gene , which can block IL-18 activity but no sequence similarity to membrane IL-18 receptors .

同时对山羊痘野生强毒H-GZ株F1~F5代细胞培养物进行检测，F1代就可检出RPHA效价，而AGP则不能检测。

Meanwhile detection was conducted with F_1-F_5 cell cultures infected by virulent virus H-GZ strain , the virus antigen could be detected in RPHA since F_1 generation and no reaction with all cell cultures in AGP .

以我国自行培养并得到广泛应用的山羊痘病毒（goatpoxvirus，GPV）疫苗株为亲本，以复制非必需胸苷激酶（tk）基因作为外源基因插入靶点及同源重组臂。

Vaccine strain of Goatpox virus ( GPV ) cultivated and widely used in domestic was employed as the mother virus . The tk gene of GPV , the nonessential region during virus replication , was chosen as target site and homologous arm when inserting foreign gene .

根据发表的羊痘病毒P32基因，设计一对特异性引物，PCR扩增P32全长基因，将其克隆入pMD-18-T载体，获得重组质粒pMD-P32。

A pair of PCR primes were designed according to the published sequence of capripoxvirus major antigen p32.The P32 gene was multiplied by PCR and cloned into the pMD-18-T vector , we got the recom - binant bacmid pMD-p32.Then the P32 gene was cloned into the E.

以山羊痘疫苗免疫绵羊防制绵羊痘的报告

A report of the use of goat pox vaccine in control sheep pox

结果表明，近年来发生在贵州省部分地区的山羊疫情是山羊痘所致。

The above-mentioned results showed that the loemia is resulted from goat pox virus .

本研究为小反刍兽疫重组山羊痘疫苗的产业化提供了参考。

This study established a foundation for the industrialization of the PPRV recombinant GPV vaccine .

三是对山羊痘病变及山羊痘病毒进行了比较详细的透射电镜观察。

The third part is observation of goat poxvirus ( GTPV ) by transmission electronic microscopy .

该诊断试剂可用于兽医临床上山羊痘的快速诊断及作为病毒分离培养的检测指标。

The reagent could be used for diagnosing goat pox quickly and working as an indicator for virus isolation and cultivation .

羊痘是由羊痘病毒引起的羊的一种急性、热性、接触性传染病，是所有动物痘病中最为重要的一种。

Capripoxvirus causes a severe and highly contagious disease in sheep or goats , it is the most important in all animal pox virus .

许多其它如出血性败血症、绵羊痘和山羊痘等可预防疾病以及许多抑制生产的疾病，如乳腺炎，都引起人们的关注。

Many other preventable diseases are of concern , including hemorrhagic septicaemia , sheep and goat pox and many production limiting diseases , such as mastitis .

研究结果表明两对引物具有羊痘病毒属特异性，不与副痘病毒属羊传染性脓疱病毒、禽痘病毒属鸡痘病毒、健康山羊皮肤样本发生交叉反应。

The results showed the primers have the specificity of Capripoxvirus and do not have consensual reaction with the Orf virus of parapoxvirus , Fowl pox virus of Avipoxvirus and the skin samples of healthy goats .

本研究室2003年以来对山羊痘病毒感染多种细胞进行了透射电镜观察。

The various cells infected by goat pox virus were observed using transmission electronic microscope since 2003.Numerous typical virus particles of goat pox in different mature stages were found in cytoplasma of the affected epithelial cells in skin and mucosa .

研究了在相同培养条件下不同培养液、不同血清和不同细胞密度对绵羊睾丸细胞生长的影响，并对该细胞接种羊痘弱毒后的病变情况进行了观察。

Effect of cell culture media , sera and cell density on growth of ovine testicular cells was studied under the identical culturing condition and pathological changes of the cells inoculated with the ( attenuated ) sheep pox virus was observed .