经典激活途径
- 网络classical pathway
经典激活途径-
补体经典激活途径C3转化酶的体外组装及活性观察
Formation and characterization of classical pathway C3 convertase of complement system in vitro
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目的体外组装包含人C4分子的补体经典激活途径C3转化酶，并对其转化酶活性及衰变特性进行观察。
Objective To prepare classical pathway C3 convertase of complement system in vitro and to observe its activity .
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结论C1、C2用量及孵育温度是影响C3转化酶形成和自发性衰变的主要因素，体外组装的补体经典激活途径C3转化酶可应用于相关补体调控蛋白的活性检测。
Conclusions gp C1 or gp C2 concentration and incubation temperature are key factors in the formation and decay of the classical pathway C3 convertase . C3 convertase prepared by this method can be used to detect DA activity of related complement regulatory proteins .