硅胶柱层析

通过聚酰胺及硅胶柱层析，利用不同的洗脱体系对巫山淫羊藿的化学成分进行分离得到单体化合物，然后利用UV、IR、NMR、MS等技术鉴定了化合物的结构。

The components were separated on polyamide and silica gel column chromatography and the structure was identified by spectral evidence of UV , IR , NMR and MS.

方法利用反复硅胶柱层析的方法对广西白背叶植物叶中的化学成分进行分离和纯化，通过IR、MS、NMR等光谱技术和化学方法鉴定化合物的结构。

Methods Silica gel column chromatography was employed to isolate and purify the constituents . Structures of the chemical constituents were elucidated by IR , MS and NMR .

精制黄酮C水解，用乙酸乙酯萃取水解液得萃取液，萃取液减压浓缩得棕褐色固体D，固体D经硅胶柱层析得两个甙元，用波谱知识鉴定其为山奈酚、槲皮素。

Two kinds of flavone aglycones were obtained from hydrolyzed refined flavone by water , through silica gel chromatography separation .

方法：应用硅胶柱层析等分离技术分离化合物，根据理化性质及IR，MS，NMR等光谱数据鉴定结构。

METHOD : By way of spectra as IR , MS and NMR .

方法：以硅胶柱层析结合薄层制备的方法，分离白术内酯等对照品，以HPLC法测定白术中活性成分的含量。

METHODS : Sesquiterpene lactones were separated by the silica gel column and layer preparation , and the active substances were determined by HPLC .

方法HPLC法确定龙血素B及近旁杂质峰位置，硅胶柱层析用于龙血素B近旁杂质峰的分离，质谱和核磁共振用于结构鉴定。

Methods Confirm the positions of loureirin B and impurity peak of loureirin B by HPLC , the substance was isolated and purified with the silica gel column chromatography .

用SephadexG50及硅胶柱层析对其进行了纯化，得率为5‰。

It was purified by Sephadex G50 / Silica column chromatography .

A部分通过反复硅胶柱层析，SephadexLH-20纯化等方法，对其中的香豆素内酯类化合物进行了分离纯化。

Coumarin of section A was isolated through silica gel column chromatography and Sephadex LH-20 repeatedly .

方法：通过分级萃取和多种硅胶柱层析（SephadexLH-20凝胶过滤、ODS反相层析和HPLC）追踪分离抗癌活性成分；

Methods : Antineoplastic compounds were screened by bioassay-guided method with partitive extraction and multiple column chromatographies ( Sephadex LH-20 , ODS and preparative HPLC ) .

方法：通过反复硅胶柱层析对黄药子氯仿部位的化学成分进行分离纯化，分别采用红外（IR）、质谱（MS）、核磁共振（包括1H-NMR，13C-NMR以及2D-NMR）方法鉴定结构；

Methods : The compounds were separated and purified by repeated column chromatography with silica gel , and identified by IR , MS , NMR and 2D NMR .

利用AB-8大孔树脂、聚酰胺、硅胶柱层析技术结合重结晶方法对燕麦黄酮进行纯化。

The oat ethanol extractions were separated and purified by AB-8 macroporous resin , polyamide and silica gel column chromatography and recrystallization method .

方法：利用硅胶柱层析和高效液相色谱进行分离纯化，采用UV、IR、MS、NMR和2DNMR波谱技术进行结构解析。

Method : Compounds were separated and purified with silica gel column and preparative HPLC , and their structures were elucidated on the basis of spectroscopic evidence ( UV , IR , MS , NMR and 2D NMR ) .

深入研究了超临界CO2萃取得到的青蒿素粗品的硅胶柱层析提纯过程的操作条件，进一步精制以提高其纯度，来满足市场需求。

Because of the low purity of artemisinin after the supercritical carbon dioxide extraction , it was thoroughly investigated that the condition of Silica gel Column chromatography for high purity of artemisinin to meet the demand of the market .

方法采用硅胶柱层析和SephadexLH-20柱层析分离化合物，运用理化性质和波谱技术确定所得化合物的结构。

Methods 5 compounds were isolated by Sephadex LH-20 and silica gel column , and their structures were identified by spectral methods and physicochemical data .

采用一种连续中压硅胶柱层析分离高纯度表没食子儿茶素没食子酸酯（EGCG）及表儿茶素没食子酸酯（ECG），原料为含量高于98%的茶多酚。

A method is described for successive purification of epigallocatechin gallate ( EGCG ) and epicatechin gallate ( ECG ) by medium-pressure silica gel column chromatography . The raw material was 98 % tea polyphenols .

首先利用离子交换树脂法及硅胶柱层析技术自一叶萩叶中，提取、纯化得到右旋一叶萩碱（dSE），为代谢研究准备了合格药物；

At first , dSE was extracted , isolated and purified by ion-exchange resin and silica gel chromatography for preparing the qualified material for metabolism research .

从稀有放线菌7310菌株（Streptomycessp.）的发酵菌丝中经溶媒萃取和硅胶柱层析初步分离出来一种活性成分，经结构鉴定为抑菌霉素（antimycins）的A2组份。

An activity component was isolated from the culture of Streptomyces sp. by solvent extraction and column chromatography , and it was identified as antimycin A2 by structure elucidation .

利用硅胶柱层析方法，提纯增效剂A1得到内（endo）N-（2-乙基）己基-1-异丙基-4-甲基二环[2,2,2]-5-辛烯-2,3-二甲酰亚胺（1）。

Synergist A 1 was purified to obtain endo N ( 2 ethyl ) hexyl 1 isopropyl 4 methylbicyclo [ 2,2,2 ] 5 octene 2,3 dicarboximide ( 1 ) with column chromatographic method .

方法：用硅胶柱层析，SephadexLH-20柱层析和核磁共振波谱方法对红树植物玉蕊茎皮乙酸乙酯萃取部位的化学成分进行了分离和结构鉴定。

Methods : Chemical constituents from the ethyl acetate extract of the stem bark of this plant were isolated by silica , Sephadex LH-20 column chromatography and identified by NMR analysis .

以80%乙醇80℃回流8h提取银杏外种皮，提取液经硅胶柱层析后得酚酸样品。

The sample was extracted with 80 % ethanol by continuous thermal reflux from Exopleura of Ginkgo biloba at 80 ℃ for 8 hours and the extract was purified by silica gel column chromatography .

采用反复的硅胶柱层析、SephadexLH-20等分离纯化手段，对荔枝核有效部位进行分离。并运用波谱学方法对其进行结构鉴定。

The repeated Silica gel column chromatography and Sephadex LH-20 etc. methods were used to separate and purify the compounds , and the spectroscopy method was used to identify the structures of the chemical compounds .

目的：研究维吾尔药VitisamurensisRupr.的化学成分。方法：利用反复硅胶柱层析进行分离，通过IR、1HNMR、13CNMR光谱鉴定化合物结构。

Objective : To Stude the chemical constituents in the fruit of Uygur traditional medicine of Vitis amurensis Rupr . Methods : The constituents were isolated repeatedly on silica gel column chromalography and their structures were identified by IR 、 1H-NMR 、 13C-NMR spectral .

方法：采用大孔树脂，硅胶柱层析，SephadexLH-20及RP-C18等技术分离纯化单体化合物，根据理化性质、波谱数据鉴定其结构。

Method : Compounds were isolated by silica gel , Sephadex LH-20 and RP-C_ ( 18 ) column chromatography and the structures were elucidated on the basis of physiochemical data and spectral characteristics .

采用有机溶剂法从仙人掌中提取皂甙化合物，用硅胶柱层析与SephadexG-75柱分别进行分离纯化，得到纯品。

The saponins were extracted from Opuntia with organic solvent and purified by silica gel column and Sephadex G-75 gel chromatography . And a method was developed for determination of the content of total saponins in Opuntia .

采用硅胶柱层析、SephadexLH-20凝胶柱层析、高速逆流色谱（HSCCC）等分离手段结合传统的萃取、重结晶等方法从白马骨的乙醇提取物中分离得到10个单体化合物和1个甾醇混合物。

Ten compounds and one sterol mixture were isolated from Serissa serissoides by various chromatographic methods such as silicon gel column chromatography , Sephadex LH-20 , high speed countercurrent chromatography ( HSCCC ), assistant with traditional methods like extraction and recrystalization .

将AZT溶于干燥二氯甲烷中，与棕榈酰氯在室温下经酯化反应制得AZTP，硅胶柱层析将产物分离，并以重结晶法精制，其纯度在99%以上。

The esterification of AZT was carried out in dry dichloromethane with palmityl chloride at room temperature . The product were chromatographed over silica gel columns and purified by means of recrystallization . The purity of product exceeded 99 % .

提取物硅胶柱层析（硅胶100～140目，柱15mm×350mm），用石油醚-乙酸乙酯（90：10）洗脱。

The extract was subjected to a silica gel column ( 100-140 mesh , 15 mm × 350 mm ) and eluted with petroleum ether - ethyl acetate ( 9 : 1 ) .

应用赭曲霉SIPI-4411的羟化酶系统对化合物坎利酮进行生物转化，通过溶剂萃取、硅胶柱层析，获得转化产物SIPI-CANR化合物的纯品。

Bio-transforming the canrenone with the enzyme system of Aspergillus ochraceus SIPI-4411 ; By solvent extraction and silica gel column chromatography , the compound SIPI-CANR was obtained .

方法：采用溶剂法、硅胶柱层析、SephadexLH-20柱层析及重结晶等方法进行成分分离及纯化，并利用UV、IR、IH-NMR、13C-NMR、MS等波谱技术鉴定提取物的化学结构。

Methods : Solvent extraction , silica gel column chromatography , Sephadex LH-20 and recrystallization were used for the isolation and purification of the constituents . The structures of the extracts were identified on the basis of spectral analysis , such as UV , IR , IH-NMR , 13C-NMR and MS.

或用硅胶柱层析分离得到较高纯度的抗氧化剂。

A special purification of the extract is attainable by chromatography .