次黄嘌呤

免疫亲和层析法分离纯化含次黄嘌呤核苷mRNA

Immunoaffinity purification of inosine-containing mRNA on column of anti-inosine antibody

次黄嘌呤和黄嘌呤与卤化镍配合物的IR光谱特征

Nickel Halide Complexes with Hypoxanthine and Xanthine by IR Spectrum

HPLC同时测定人尿中假尿苷次黄嘌呤含量

Determination on Concentration of Pseudouridine and Hypoxanthine in Human Serum by HPLC

HPLC同步测定肌酸、磷酸肌酸、腺苷酸及次黄嘌呤类物质

Synchronous Determination of Creatine Compounds , Adenine Nucleotides , Hypoxanthine and Inosine with HPLC

以次黄嘌呤为考察指标，建立了水蛭注射液的HPLC指纹图谱。

HPLC fingerprint of hirudo injection was established , using hypoxanthine as the target .

以上结果表明，应用本方法可以有效分离得到含次黄嘌呤核苷的mRNA。

The results indicated that inosine containing mRNA could be successfully isolated from mRNA by using immunoaffinity chromatograph .

老龄小鼠肝线粒体外膜MAO提取物中次黄嘌呤含量明显低于年青小鼠，但两组MAO中腺嘌呤含量却无明显差异。

The amount of hypoxanthine , released from the MAO of aged mice , was lower than that of the young mice .

在~3H-尿嘧啶核苷和~3H-次黄嘌呤参入实验中，三尖杉酯碱对四种~3H-核苷三磷酸及RNA的合成无明显影响。

It was also noted that harringtonine showed no significant effect on the incorporation of ~ 3H-UR and ~ 3H-HX into the nucleotide triphosphates and on RNA synthesis .

ADARs是一种RNA编辑酶，作用于双链RNA（dsRNA）上的腺嘌呤，通过水解脱氨作用使腺嘌呤转变为次黄嘌呤。

ADARs are RNA editing enzymes that change adenosines to inosines via a hydrolytic deamination reaction on double - stranded ( ds ) RNA .

NADH使得次黄嘌呤黄嘌呤氧化酶体系和Fe++H2O2体系的ESR信号明显减弱甚至消失；

ESR spectrum of hypoxanthine-xanthine oxidase and Fe + + & H 2O 2 system were significantly reduced or disappear by NADH .

次黄嘌呤黄嘌呤氧化酶（HXXO）是经典的产自由基体系。

Hypoxanthine - xanthine oxidase ( HX-XO ) is a typical system for generating free radicals .

用多核细胞法检测细胞次黄嘌呤鸟嘌呤核糖转移酶（HPRT）基因突变率；

Multinuclear cell assay was used to detect the frequency of the HPRT gene mutation ;

冷藏期间三甲胺，次黄嘌呤含量随着冷藏时间延长而升高，三甲胺含量＜20μg／g，次黄嘌呤含量增加在50%以内鱼肉保持新鲜；

The contents of trimethylamine and hypoxanthine increased during cold storage . When the trimethylamine content was still under 20 μ g / g and the hypoxanthine content increase below 50 % , the fish still kept fresh .

MPA的主要作用机理是抑制次黄嘌呤单核苷酸脱氢酶（IMPDH），该酶是鸟嘌呤核苷酸从头合成途径的限速酶。IMPDH受抑导致鸟嘌呤核苷酸生成减少，进而DNA、RNA合成受阻。

The main mechanism of MPA is that it inhibits IMPDH which is the rate-limiting enzyme in de novo synthesis of guanosine nucleotides .

次黄嘌呤和黄嘌呤氧化酶反应产生的含氧自由基可以引起IAR20肝细胞SCE增高，而GTE可明显抑制这种自由基对细胞SCE的诱导作用。

GTE decreased the frequency of SCE induced by oxygen radical in IAR 20 liver cells treated with hypo-zanthine and xanthine oxydase .

应用RT-PCR法检测各实验组腺苷激酶（AK）和次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）基因转录产物含量的变化。

RT-PCR was used to examine the gene transcripts concentrations of adenosine kinase ( AK ) and hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ) in each group .

给小鼠一次po次黄嘌呤500mg/kg，于给药后16h，对MAO抑制作用最明显。

When mice were given orally hypoxanthine 500 mg / kg , MAO-A and-B activities were all inhibited significantly 16 hours after administration , but the inhibitory action on MAO-A was weaker .

运用核苷酸补救途径理论，采用枯草杆菌肌苷产生菌，在培养过程中添加次黄嘌呤，作为从头合成途径（denovo）的补充。

Applying the theory of nucleotide salvage , we added the hypoxanthine to culture process of inosine producing mutants of Bacillus subtilis as supplement of de novo formation of inosine which made inosine synthesis improved greatly .

利用猪卵母细胞体外无血清培养技术，研究了次黄嘌呤（HX）对猪卵母细胞体外自发成熟的抑制作用。

Using the serum-free culture technique for pig oocyte , the inhibitory effect of hypoxanthine ( HX ) on the spontaneous meiotic maturation of porcine oocytes was investigated .

结果采用灌胃给予次黄嘌呤，皮下注射以羊毛脂石蜡油为溶剂配制的尿酸酶抑制剂，可使受试动物血尿酸值明显高于空白对照组（P<0.01），且可维持12h。

Results Hypoxanthine was administered via stomach , while uricase inhibitor was injected subcutaneously by lanolin mixed with olefin , which made the uric acid in serum higher than control group about 12 hours .

本文采用改良的次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）试验，观察人皮角朊细胞铅离子体外染毒的遗传毒性。

An improved HGPRT assay system was used to investigate the genotoxicity of lead in human cutaneous keratinocytes exposed to the metal ion in vitro .

研究背景：腺苷脱氨酶（AdenosineDeaminase，ADA）是一种核酸分解代谢关键酶，可特异性催化腺嘌呤核苷产生不可逆脱氨反应，生成次黄嘌呤，最终氧化成尿酸排出体外。

Background : Adenosine deaminase ( ADA ) is an important enzyme of purine nucleotides catabolism . It specially catalyzes the unreversible deamination of adenine nucleoside to inosine . The inosine is then oxidized to uric acid .

大多数酶将腺嘌呤脱氨产物次黄嘌呤识别成为鸟嘌呤，在翻译过程中次黄嘌呤被当作鸟嘌呤，这样ADARs就改变了RNA的原始序列信息。

After editing , inosine ( I ) is translated as guanosine ( G ), and most enzymes recognize inosine as guanosine . Thus , ADARs change the primary sequence information in a RNA molecule .

次黄嘌呤鸟嘌呤磷酸核糖转移酶（Hprt）参与嘌呤核苷酸的补救合成。恶性疟原虫次黄嘌呤鸟嘌呤黄嘌呤磷酸核糖转移酶细胞免疫保护作用的研究

The enzyme hypoxanthine-guanine phosphoribosyltransferase ( HPRT ) plays an important role in the purine salvage pathways . Evaluation of Cell-mediated Protective Immunity to Hypoxanthine Guanine Xanthine Phosphoribosyl Transferase ( HGXPRT ) of Plasmodium Falciparum

RNA编辑是近年来发现的一种遗传信息修饰机制，它通过对前RNA特异位点的修饰，使腺嘌呤核苷转化为次黄嘌呤核苷（Adenosine-to-inosine，A-to-Ⅰ），从而在转录后水平对目的基因进行修饰。

RNA editing is a kind of genetic decoration mechnism studied in recent years . It edits special position of pre-RNA , changing adenosine to inosine ( Adenosine-to-inosine , A-to-I ) , and then decorates target gene at transcription level .

次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）基因在单基因突变的研究中是一个经典的基因位点。

Hypoxanthine guanine Phosphoribosyl Transferase ( HPRT ) is a classical gene locus in the research of single gene mutation . The principle of HPRT locus mutation detection is summarized and some methods of detection are presented .

以镍铬合金为基体制备了嵌入式超薄碳糊膜电极，研究了黄嘌呤（Xa）和次黄嘌呤（Hxa）在该电极上的电化学行为。

A novel super-thin carbon paste film ( ISTCPF ) electrode prepared by inlaying carbon paste onto the surface of nickel-chromium substrate was described , and the properties of the electrode were investigated .

以黄嘌呤氧化酶作用于次黄嘌呤而产生氧自由基，观察其对体外培养小母牛肺动脉内皮细胞（CPAE）的增殖和分泌血管紧张素Ⅱ（ATⅡ）的影响。

The growth and secretion of angiotensin ⅱ of cultured endothelial cells of pulmonary artery of female young cow ( CPAE ) stimulated by hypoxanthine plus xanthine oxydase were observed .

方法从玄参根中分离提取苯丙素苷成分Acteoside，采用次黄嘌呤造成小鼠高尿酸血症，观察Acteoside对小鼠高尿酸血症的影响，并进一步从体外观察Acteoside对黄嘌呤氧化酶的抑制作用。

Methods Acteoside was extracted from roots of Scrophularia ningpoensis . A mouse hyperuricemia model was established by using hypoxanthine and the effect of acteoside on hyperuricemia in mouse was observed before the inhibition effect of acteoside on xanthine oxidase in vitro was further studied .

次黄嘌呤对昆明白小鼠卵母细胞减数分裂调控作用的研究

Studies on Hypoxanthine Control of Meiosis of Oocytes of Kunming Mice