梯度洗脱

流动相：A相为乙腈，B相为水，梯度洗脱；

Mobile phase was A : ACN , B : H _ 2O , gradient elution ;

HPLC梯度洗脱法检查甲睾酮喷雾剂中的有关物质

Determination of related substances in Methyltestosterone spray by HPLC gradient elution

流动相：0·1%甲酸水溶液（A）-乙腈（B），梯度洗脱；

Mobile phase : 0.1 % formic acid-water ( A ) and acetonitrile ( B ), gradient elute ;

梯度洗脱HPLC法测定盐酸平阳霉素及注射药物中的有关物质

Determination of related substances in bleomycin A5 hydrochloride by gradient elution HPLC

HPLC梯度洗脱法同时测定人血浆中对乙酰氨基酚和维生素C浓度

Simultaneous Determination of Paracetamol and Vitamin C in Human Plasma by HPLC Method with Gradient Elution

考察了梯度洗脱分离时，起始缓冲液组成及pH值对分离的影响。

The effects of initial buffer composition and pH on the gradient elution have been investigated .

HPLC梯度洗脱法测定防风中4种主要成分含量

Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution

方法：采用HPLC梯度洗脱法进行测定。

Methods : The determination was carried out using HPLC gradient elution method .

类胡萝卜素亦采用线性梯度洗脱，以乙酸乙酯作为A相，B相为乙腈-水（体积比9∶1）。保留时间、相对保留时间、光谱法、加标定性，内标法定量。

Carotenoids were separated by linear gradient elution using ethyl acetate and water-acetonitrile ( 1:9 , V / V ) as mobile phase .

选择优化的HPLC色谱条件，用梯度洗脱法分离测定原药及注射液中鲑鱼降钙素的含量。

A gradient HPLC method was developed for the determination of calcitonin and its content in injection .

方法RP-HPLC离子对梯度洗脱法。

Method Ion-pairs along with gradient elution HPLC method was proposed .

山茱萸果汁RP-HPLC梯度洗脱优化实验研究

Analysis of Cornus officinalis juice by RP-HPLC with gradient elution

HPLC梯度洗脱法测定beagle犬血浆中阿魏酸钠的含量

Determination of content of sodium ferulate in beagle plasma by HPLC gradient elution method

方法利用RP-HPLC方法，梯度洗脱测定10批样品。

Methods HPLC was utilized for quality assessment of 10 batches of samples .

方法：HPLC梯度洗脱方法测定广炎灵粉针中丁香苦苷A的含量。

Methods : The syringopicroside A was determined in GuangYanling Powder injection by HPLC gradient elution GuangYanling Powder injection analysis method .

方法：采用HPLC梯度洗脱法、电感藕合等离子体原子发射光谱法（ICP-AES）分别测定了补骨脂炮制前后的补骨脂素、异补骨脂素及六种微量元素的含量。

Methods : The content of psoralen , isopsoralen and microelement were obtained by HPLC-gradient elution and ICP-AES .

RP-HPLC梯度洗脱法测定泮托拉唑钠的有关物质及含量

RP-HPLC gradient elution analysis for the determination of pantoprazole soldium and its related substances

目的建立两种HPLC线性梯度洗脱测定阿莫西林/克拉维酸钾有关物质的方法，并对其进行比较。

Objective Two HPLC methods with linear gradient elution for determination of the related substances of amoxicillin-potassium clavulanate were established and compared .

在此基础上进一步对体内代谢进行研究，采用梯度洗脱HPLC法分析大鼠胆汁、尿和血浆中氨丁苯酞及其代谢物。

In-vivo metabolism studies were carried out by analysis of metabolites in rat bile , urine and plasma with gradient HPLC method .

方法：以毛蕊花糖苷为参照物，采用梯度洗脱HPLC法。

Methods : With acteoside as the reference substance , gradient elution HPLC method was applied to establish fingerprint chromatography of Cistanche deserticola .

目的：研究应用反相离子对色谱梯度洗脱法以较快速度分离硫酸博莱霉素组分，并尝试梯度洗脱条件下测定其有效成分A2、B2的含量。

Objective : To develop a HPLC linear gradient elution method for rapid separation of the components of bleomycin , and for determination of bleomycin A2 and bleomycin B2 . Method ;

RP-HPLC梯度洗脱法测定杏香兔耳风不同部位中绿原酸和芦丁的含量

Determination of Rutin and Chlorogenic Acid in Different Parts of Ainsliaea Fragrans by HPLC with Gradient Elution

梯度洗脱；流速1.0ml／min；

Gradient elution with flow rate of 1.00ml / min ;

采用离子交换-梯度洗脱体系，对废铬液14次循环利用过程中鞣液铬配合物组分与pH值之间的关系，进行了跟踪测定。

A cation exchange resin associated with the elution system was used to determinate effect of pH on chromium complexes in spent and regulated liquors during 14 times of tanning recycle .

方法采用RP-HPLC法，梯度洗脱，研究决明子饮片的指纹图谱，并作相似度比较分析。

MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation .

流动相B：乙酸乙酯；线性梯度洗脱：在前15min内，B由0增为100%，随后，B保持100%；

Linear binary elution gradient : mobile phase B increased from 0 to 100 % in the first 15 minutes and later kept 100 % consequently ;

大豆异黄酮的AB-8树脂吸附梯度洗脱纯化精制工艺研究

Refining Isoflavone by AB - 8 Resin Absorption and Terraced Concentration Desorption

方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。

Method The main ingredient of the injection , Red Ginseng , was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng .

流动相为乙腈-0.5%冰醋酸水溶液，采用梯度洗脱程序，流速为1.0mL／min；

The mobile phase was with acetonitrile-0 . 5 % acetic acid solution in program of elution ;

对弱结合的金属螯合柱可用低浓度盐或pH梯度洗脱，对强结合的金属螯合柱通常用竞争洗脱剂洗脱。

The gradient elution is generally performed by the low concentration of salt or different pH for weakly combined columns with proteins , however the competitive elution procedure is commonly utilized for strongly combined column .