异硫氰酸胍

用异硫氰酸胍法提取鱼油、加入牛肉DNA的鱼油、加入山羊肉DNA的鱼油和牛油中的DNA。

The total DNAs of fish oil , fish oil added with bovine DNA , fish oil added with goat DNA and butter were extracted by guanidine isothiocyanate method .

结果表明，由异硫氰酸胍法、SDS法和Tris-硼酸法3种方法获得的RNA纯度较低，存在降解和弥散现象，或混杂的DNA、蛋白质较多，效果均不理想；

The results indicated that all the RNA isolated by guanidine isothiocyanate method , SDS method and Tris-borated method were degraded and dispersed in some degrees .

研磨-CTAB法与碱性异硫氰酸胍沸腾法提取真菌DNA的比较分析

Compare Analysis of CTAB method with alkaline guanidine thiocyanate boiling method for extraction of fungal genomic DNA

异硫氰酸胍法提取大鼠睾丸总RNA及质量鉴定

Guanidinium thiocyanate method to isolate RNA from rat testis and the quality control

改良异硫氰酸胍一步法提取红豆杉细胞RNA

Improved single-step method for extracting total RNA from Taxus cells with guanidinium isothiocyanate

异硫氰酸胍法提取梅花鹿鹿茸组织总RNA

Extraction of Total RNA from Velvet Antler of Sika Deer with Isothiocyanate Method

用改进的异硫氰酸胍一步法提取完熟水果RNA

The Improved Single - Step Method to Isolate RNA from Ripening Fruit with Guanidinium Isothiocyanate

用异硫氰酸胍与β-巯基乙醇联合变性的方法，从鸡脾脏中提取总RNA。

The total RNA from chicken spleen cells was extracted with the guanidine thiocyanate and β - mercaptoethanol .

结果表明：改进异硫氰酸胍法获得的总RNA纯度高、完整性好。

The result showed that the total RNAs obtained by Improved Guanidine Thiocyanate method were highly pure and undegraded .

方法采用异硫氰酸胍法抽提皮肤组织中的RNA；

Methods The RNA of skin tissue was extracted using single-step method of RNA isolation by acid guanidinium thiocyanate .

以白菜花药组织为试材，利用异硫氰酸胍法和改良Tris-硼酸法提取花药总RNA。

Guanidine thiocyante method and modified Tris-borate method were employed to isolation total RNA from anthers of non-heading Chinese cabbage .

异硫氰酸胍法提取转基因生菜的RNA进行RT-PCR检测，结果3株均能够扩增出特异性条带，这初步表明胸腺肽基因已经在转录水平上表达。

RNA of transgenic lettuce was extracted and RT-PCR detection preliminarily proved that the thymosin gene was expressed in transcriptional level .

本试验采用异硫氰酸胍“一步法”和CTAB法提取甘薯块根总RNA，并对改进前后两种方法提取的总RNA产量和纯度进行了比较。

Guanidine Thiocyanate-one step method and CTAB method were used to isolate the total RNA from tubers of sweet potato .

结果显示两种方法提取RNA的产量都较高，异硫氰酸胍法提取纯度低，但省时高效，适用于对模板要求低的RNA提取。

Results showed that both the methods produced high quantity of RNA . Guanidine thiocyante method produced RNA with low purity but more time-consuming .

该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总RNA完整性好和纯度高等优点。

The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods .

该方法用异硫氰酸胍提取进口饲料样品DNA，以牛特异性引物扩增模板DNA，扩增产物为271bp，PCR产物经限制性酶切片段分析，与预期设想一致；

DNAs were isolated from import feedstuff samples by guanidine isothiocyanate method . Bovine specific primers were synthesized and applied in the amplification and the length of target product was 271 bp .

方法：用异硫氰酸胍一步法提取病毒RNA，经RT－PCR扩增NS4A基因片段。

Methods : After isolation of viral total RNA with guanidinium method , the objective gene was amplified using RT-PCR .

结果：进一步改进的异硫氰酸胍法能够提取到质量高（OD260/OD280的值介于1.8～2.0）、完整性好的总RNA，且将提取时间缩短为原来的一半（全程仅需2～3小时）。

Results : With the modified Guanidine Thiocyanate method , high-quality and good-integrality total RNA was achieved and the cost time period for the isolation of RNA was sharply shortened .

通过改进的异硫氰酸胍法提取香蕉分化芽中的总RNA，既经济又满足了RT-PCR的要求。

It is not only economic but also capable of meeting the requirements of RT-PCR to extract the total RNA from the in vitro differentiated shoots of banana by the modified Guanidine Thiocyanate method .

从RNA的完整性、产率和纯度等方面对这三种方法进行了比较和评价，结果发现改进的异硫氰酸胍法是从大豆胚芽中提取RNA的适宜方法。

The three methods are evaluated by determinating quality , purity and integrity of RNA sample and the results suggest that the modified guanidine thiocyanate method is more fit for extracting total RNA from soybean embryo .

采取异硫氰酸胍法从黄河鲶肌肉、血液、全鱼、肾脏、肝脏、卵巢中提取出完整RNA，结果表明，通过该方法所提取RNA质量高，效果好，可以用于进一步的分子生物学研究。

Guanidine Thiocyanate is ideal for extracting and purifying RNA of the Yellow River catfish . Through this method , RNA of high quality had been obtained from muscle , blood , kidney , liver , ovary , which can be used for the further molecular biology research .

方法异硫氰酸胍裂解、酚-氯仿抽提、异丙醇沉淀法直接抽提结核分枝杆菌rRNA，地高辛标记探针斑点杂交，酶呈色观察结果检测结核分枝杆菌，用该法检测76例临床标本。

Methods To extract the rRNA of mycobacterium tuberculosis by guanidine thiocyanate splitting , phenol-chloroform extraction , isopropanol precipitating and proceeding dot-blotting with the probe emblematized by Digoxin , observing the results by enzyme presents color . Seventy-six clinical isolates were detected by this method .

在高浓度离液剂异硫氰酸胍（GuSCN）存在的条件下，核酸具有与硅珠（SiO2）颗粒结合、并能在较高温度下被低盐浓度溶液洗脱的特性。

Based on the presence of high concentrations of the chaotropic agent guanidinium thiocyanate ( GuSCN ), the nucleic acid could bind to silica particles ( SiO_2 ) and subsequently was eluted in a aqueous low-salt buffer .