基因置换

利用PCR介导的基因置换技术构建阿维链霉菌bkdF~-突变株

Construction of Streptomyces avermitilis bkdF ~ - Mutants Using PCR-mediated Gene Replacement Method

PCR介导的基因置换技术是一种利用λ噬菌体Red重组系统在微生物中进行基因中断的新方法。

PCR mediated gene replacement method is a novel way of gene knockout in bacteria which is based on λ Red system .

卡介苗ERP基因置换型打靶载体的构建

Construction of the gene targeting vector of ERP gene in BCG

结论构建了卡介苗ERP基因置换型打靶载体，并构建筛选卡介苗ERP基因缺失突变株。

Conclusion Constructing the replacement vector which is used to the gene knockout in BCG . constructing the BCG mutation with deleted ERP gene .

同时对发酵产物HPLC分析条件进行了摸索，并构建了基因置换和缺失突变株对其生物合成途径进行初步研究。

The HPLC analysis conditions of the fermentation product were explored at the same time . Gene replacement and in frame deletion mutants was constructed for preliminary study of its biosynthetic pathway .

运动发酵单胞菌adhB基因置换变形链球菌Idh基因重组质粒的构建

Construction of Recombinant Plasmid Through Substituting the Alcohol Dehydrogenase ⅱ Gene of Zymomonas Mobilis for the Lactate Dehydrogenase Gene of Streptococcus Mutans

本文对基因置换技术实现局部搜索的机理进行了分析和描述。

The local search mechanism of gene replacement is analyzed and described .

用健康基因置换那些胎儿发育时功能就不正常的基因时效果尤其不佳。

It will be particularly difficult to compensate for genes that malfunction during fetal development .

与此同时，对基因置换操作实现局部搜索的计算代价进行分析估计，指出该操作函数值计算次数上限和经验估计。

At the same time , the computation cost for the operation of gene replacement to perform local search is analyzed and estimated and the upper limit of iteration number is given . 5 .

对于二进制编码遗传算法所出现的海明悬崖现象，根据基因置换完毕的编码特征，提出识别和修复方法，从而可以确保算法实现二进制编码的最高计算精度。

According to the characteristics of the codes after gene replacement , the technique to cope with the Hamming cliff phenomenon often emerging in binary encoded GA is proposed , and this guarantees that BA can achieve the upmost accuracy of computation under binary encoding .

我们知道在大自然中基因相互置换。

In nature we know that genes interchange with each other .

基因打靶置换型载体的构建和应用研究

Construction and application of gene targeting replacement vector

6株毒株N基因发生氨基酸置换，3株毒株发生缺失变异；

Those domains in 6 stains in N gene were substituted and 3 strains varied in deletion of nucleotide .

结论：成功构建了用于结核分支杆菌基因打靶的置换型载体，为随后将进行的Rv0901基因敲除株的建立，Rv0901基因功能的研究奠定了基础。

Conclusion : Constructing successfully the replacement vector which is used to the gene knockout in Mycobacterium Tuberculosis .

SNP基因芯片结合多重置换扩增技术检测单细胞非整倍体的研究混合法用于基因芯片数据的分类分析

Detection of Aneuploidy from Single Cells by Array-Comparative Genetic Hybridization A Combined Method Applied to the Classification of Microarray Gene Expression Data

从链霉菌FR－008中克隆出一个七烯大环内酯抗生素的生物合成基因簇，基因置换试验证实克隆的基因片段与抗生素生物合成直接相关。

Genes for biosynthesis of a Streptomyces sp. FR-008 heptaene macrolide antibiotic faith antifungal and mosquito larvacidal activity were cloned in E.

载脂蛋白AⅠ基因启动子G/A置换多态性和饮酒的联合作用对凉山地区彝汉人群血浆载脂蛋白AⅠ水平的影响

Influence of a G / A substitution at - 75 bp promoter region of apolipoprotein A ⅰ gene and wine consumption on the plasma apolipoprotein A ⅰ levels in Yi and Han Chinese

基因工程（GE）主要是将两种没有亲缘关系的物种进行基因置换以得到新型物种。

Genetic engineering ( GE ) involves exchanging genes between unrelated species that cannot naturally exchange genes with each other .

分别用绿色荧光蛋白（GFP）基因、β葡糖醛酸酶（GUS）基因以及小鼠二氢叶酸还原酶（DHFR）基因3种报告基因置换CP基因。

The CP gene was replaced by green fluorescent protein ( GFP ) gene ,β glucuronidase ( GUS ) gene or mouse dihydrofolate reductase ( DHFR ) gene , respectively .