变性凝胶电泳

提取总RNA，用紫外分光光度计法检测RNA的纯度，并取总RNA样品进行甲醛变性凝胶电泳，检测总RNA完整性。

Total RNA was extracted , then its purity was detected by ultraviolet spectrophotometer , and its integrity was tested by taking the total RNA samples to RNA formaldehyde denaturing gel electrophoresis . 4 .

根据RNA在A260/280值≥1.7，以及甲醛变性凝胶电泳28s、18SRRNA条带清晰，亮度比值接近2：1（见图9），满足miRNA芯片实验的要求。

According to RNA at A260 / 280 value ≥ 1.7 , as well as formaldehyde denaturing gel electrophoresis , 28s , 18s rRNA bands clarity , brightness ratio near 2:1 , to meet the requirements of miRNA chip experiments . 3 .

变性凝胶电泳和自动DNA序列分析检测结直肠肝转移癌p53基因突变的研究

Study the detection of p53 gene mutation on colorectal cancer and liver metastasis by denaturing gradient gel electrophoresis and automated sequencing

得到的RNA样品经过紫外分光光度法和甲醛变性凝胶电泳检测，证实为完整、均一的总RNA，为构建真菌的cDNA文库和筛选差异表达的基因奠定了基础。

The integrity and purification of the total RNA isolated are eligible by using formaldehyde-a-garose gel electrophoresis and UV spectrophotometer . It is useful in constructing cDNA library for the total RNA and also valuable in screening differential expressed genes .

方法：在这研究中，我们将会探讨：（1）非变性凝胶电泳和（2）毛细管区带电泳（CZE）。

Methods : In this paper , it will study on : ( 1 ) non-denaturing gel electrophoresis and ( 2 ) capillary zone electrophoresis ( CZE ) .

甲醛变性凝胶电泳显示，各样品电泳图谱有清晰的28S、18S条带和一条浅的5S条带，且肉眼观察28S条带亮度约为18S的1.5倍。

Formaldehyde denaturing gel electrophoresis showed that all samples have clear 28S and 18S bands and a weak 5S strip . And the band of 28S is 1.5 times the intensity of 18S by visual observation . 3 .

研究蛋白质-蛋白质相互作用，由本地和变性凝胶电泳免疫印迹，免疫沉淀。

Study protein-protein interaction by native and denaturing gel electrophoresis , western blotting , and immunology precipitation .

通过液氮研磨提取甘薯愈伤组织和块根可溶性提取物，并采用非变性凝胶电泳和淀粉酶活性染色方法的研究发现，甘薯愈伤组织有4种迁移率不同的淀粉酶活性带，而块根只有1条淀粉酶活性带。

Amylase isozyme composition of calli and tuberous roots from sweet potato was analyzed by native PAGE and active staining , using amylopectin or soluble starch as substrate .

结果表明，非变性凝胶电泳法和紫外分光光度法可用于环丙沙星与卵清蛋白和牛血清白蛋白分子结合比的定性分析和定量分析。

The results indicate that nondenaturing gel electrophoresis and ultraviolet spectrophotometry can be employed to analyze the molecule coupling ratio of CPFX to carrier proteins qualitatively and quantitatively .

变性梯度凝胶电泳装置及其在DNA突变检测中的初步应用

Denaturing gradient gel electrophoresis system used in mutations detecting

用变性梯度凝胶电泳分析PCR克隆的突变率

Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis

方法组织DNA提取后PCR扩增，扩增产物行6%变性聚丙烯酰胺凝胶电泳，最后银染。

PCR products were run on 6 % denaturing polyacrylamide gel and stained with silver .

PCR产物由非变性聚丙烯酰胺凝胶电泳分析。

The PCR products were analyzed by PAGE electrophoresis .

变性梯度凝胶电泳检测到四例新的FⅨ基因突变

Four novel point mutations of factor ⅸ gene detected by denaturing gradient gel electrophoresis

方法：选用不同pH的正极缓冲液和样品蛋白上样量，应用非变性聚丙烯酰胺凝胶电泳分离大鼠海马组织蛋白质。

Methods : Nondeture PAGE was used to separate proteins in different pH and loading quantity of protein sample .

用变性聚丙烯酰胺凝胶电泳检测SSR扩增产物结果更准确。

The detection effects of denaturing polyacrylamide gel electro-phoresis were more accurate .

选取10例鸡马立克肿瘤组织及同源正常组织，抽提DNA后PCR扩增，琼脂糖电泳初步筛选、变性聚丙烯酰胺凝胶电泳后观察结果。

Ten samples of Marek 's tumor and homological normal tissues were taken , and amplified by PCR after extraction of DNA .

方法用PCR扩增、非变性聚丙烯酰胺凝胶电泳分型；

Methods Two Y-STR loci were amplified with PCR . The products of PCR were analyzed with polyacrylamide gel electrophoresis .

体外转录生成核酶和靶RNA分子，进行切割反应后经变性聚丙烯酰胺凝胶电泳，银染法显色判定结果。

After in vitro cleaving reactions polyacrylamide gel electrophoresis was carried out followed by the silver nitrate staining .

应用PCR扩增微卫星标志，变性聚丙烯酰胺凝胶电泳分析各个微卫星标志的LOH状况。

Microsatellite markers amplified by PCR were detected for LOH by denatured PAGE .

AR基因产物经变性聚丙烯酰胺凝胶电泳、银染显示其长度多态性；

The length polymorphism of AR gene was demonstrated by denaturing polyacrylamide gel electrophoresis and silver staining .

利用变性梯度凝胶电泳、克隆和实时PCR等分子生物学技术对2个厌氧氨氧化反应器中的微生物进行了初步研究。

The molecule biological techniques DGGE , clone and real-time PCR were utilized to study prinimilarily the microorganism in2 anaerobic ammonia oxidation reactors .

扩增产物经过6%变性聚丙烯酰胺凝胶电泳后银染，结果2只体细胞克隆山羊的微卫星DNA指纹与供体细胞完全相同，而且不同于其受体母亲也不同于其他所有同品种不同个体的对照青山羊。

The microsatellite DNA fingerprints of the two somatic cloned goats are the same as the donor , but are different from the recipient goat and all the control grey goats .

设计了一套适用于变性梯度凝胶电泳（DGGE）的装置。

A denaturing gradient gel electrophoresis ( DGGE ) system was designed .

应用变性梯度凝胶电泳技术和16Srdna序列分析技术研究山羊瘤胃细菌的多样性

Bacterial Diversity in Goat Rumen as Characterized by Denaturing Gradient Gel electrophoresis and 16S rDNA Sequence Analysis

方法用PCR变性聚丙烯酰胺凝胶电泳银染法，检测50例肺癌组织Bax和TGFβRⅡ基因移码突变及微卫星改变。

Methods Frameshift mutations and microsatellite alterations were detected in 50 primary lung cancer tissues by PCR , 8 % denature polyacrylamide gel electrophoresis and silver staining .

方法：用PCR、变性梯度凝胶电泳（DGGE）和DNA测序检测因子Ⅷ基因突变。

Methods : PCR , denaturing gradient gel electrophoresis ( DGGE ) and DNA sequencing were used to screen mutations in the factor ⅷ gene .

粪样细菌区系的变性梯度凝胶电泳（DGGE）分析；

Analysis of calves ' excrement with Denaturing Gradient Get Electrophoresis ( DGGE );

并采用分子生物学技术聚合酶链式反应&变性梯度凝胶电泳（POLYMERASECHAINREACTION-denaturinggradientgeleleetrophoresis，PCR-DGGE）对污泥微生物群落的演替展开研究。

The microbial community structures and succession were studied by polymerase chain reaction-denaturing gradient gel eleetrophoresis ( PCR-DGGE ) .

为此，在介绍变性梯度凝胶电泳（DGGE）以及荧光原位杂交（FISH）等分子生物学新技术的基础上，对这些技术在土壤微生态研究中存在的问题和发展前景做了展望；

In this paper , several molecular methods such as fluorescence in situ hybridization ( FISH ) and denaturing gradual gel electrophoresis ( DGGE ) etc.