区带离心
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- 名zonal centrifugation
区带离心
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大规模区带离心纯化Vero细胞乙脑疫苗
Large Scale Purification of Inactivated Japanese Encephalitis Vaccine from Vero Cells by Zonal Centrifugation
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第三步用溴化钾作漂浮密度梯度区带离心,和第四步用蔗糖密度梯度速率区带纯化所得的HBsAg光密度单位剩余为0.32%,也就是去除了99.68%杂蛋白,回收率为73.4%。
Further purification was done by KBr buoyant-zonal density gradient centrifugation , and sucrose rate-zonal density gradient . The obtained HBsAg optic density unit of the remainder was 0.32 % , i. e. 99.68 % of other proteins were removed .
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本文采用区带密度梯度离心,国内首次分离得到黄牛纯化的高密度脂蛋白(HDL)。
The bovine high density lipoprotein ( HDL ) was first isolated and purified in our country with zonal gradient ultracentrifugation .