功能基因组学

DNA多态性及其在植物功能基因组学研究中的应用

DNA Polymorphism and Its Applications in Plant Functional Genomics

BOAT可能是功能基因组学研究的一种新工具。

BOAT can be a useful new tool for functional genomics studies .

RNA干扰文库在功能基因组学研究中的发展及应用

Development and Application of RNAi Interference Library in Functional Genomics

RNA干涉技术在功能基因组学和医学研究中的应用

RNA Interference in Functional Genomics and Medical Research

cDNA微阵列是当前功能基因组学研究中的一种强有力的工具。

CDNA microarray is the most powerful tool in the research field of functional genomics .

并分析了功能基因组学的研究及RNA干扰技术在黑麦草研究中可能的应用前景。

Furthermore , the prospects of RNA interference in Lolium genetic improvement and functional genomics are discussed .

这为罗汉果功能基因组学以及甜苷V生物合成分子机理研究打下了坚实的基础。

This is a solid foundation for the study of Siraitia grosvenorii functional genomics and mogrosides V molecular mechanism of biosynthesis .

2000年被发现以后，RNA干扰被迅速应用于功能基因组学以及病毒感染、肿瘤以及遗传病的基因治疗等领域。

Since it was found in 2000 , RNAi has been used in functional genomics , gene therapy and antiviral research .

单倍体再用秋水仙碱处理后获得DH系，所得材料对油菜功能基因组学的研究可能有一定的价值。

We generated completely homozygous DH lines as a model system for functional genomics work in B.

近年来植物功能基因组学的研究技术主要包括表达序列标签、基因表达的系列分析、DNA微阵列和反向遗传学等。

In recent years , main methods of studying the plant functional genomics include expressed sequence tag , serial analysis of gene expression , DNA micro array , reverse genetics and so on .

功能基因组学（functionalgenomics）是利用结构基因组所提供的信息和产物，发展和应用新的实验手段，在基因组或系统水平上全面分析基因的功能。

Introducion : Functional Genomics is to analyze the function of genes systematically by using the information of structural genomics , developing and utilizing new experimental tools .

植物功能基因组学研究是利用结构基因组学积累的数据，从中得到有价值的信息，阐述DNA序列的功能，从而对所有基因如何行使其职能并控制各种生命现象的问题作出回答。

That plant functional genomics study is to give an answer to how gene controls various biological phenomena , obtaining valuable information from the data of structure genomics and expounding the function of DNA sequence .

Mu插入突变体库的构建与遗传评价为进一步利用Mu转座子发掘玉米基因，深入开展玉米功能基因组学研究搭建重要的技术与材料平台。

The mutator insertional mutants pool in maize constructed in the current research will provide a valuable platform for gene discovery and functional genomics study in maize .

构建靶向大量基因的RNA干涉文库在基因组范围内实现基因的沉默，已成为研究细胞生物学行为的一种新的功能基因组学研究方法。

The preparation of RNA interference library targeting a large number of genes to globally knock down expression of genes on genome-wide scale has become a new approach to study cellular biological behaviors .

TILLING技术在功能基因组学中的应用

TILLING for Functional Genomics

RNAi技术在疾病的基因治疗、功能基因组学及细胞信号通路分析等力面具有广阔的应用前景。

RNAi has made a wide application in disease gene therapy , functional genomics and cell signaling pathway analysis , to name just a few .

植物功能基因组学的研究方法主要包括：表达序列标签（EST）、基因表达序列分析（SAGE）c、DNA微阵列和DNA芯片、反求遗传学、蛋白质组学和生物信息学；

The research metheds of functional genomics , mainly include Expressed Sequence Tag ( EST ), Serial Analysis of Gene Expression ( SAGE ), cDNA microarrays and DNA chips , Reverse genetics , Proteomics and Bioinformatics .

RNAi可作为一种简单有效的代替基因剔除的工具应用于功能基因组学和疾病的基因治疗研究。

As a complement of gene knock-out , RNAi works as a simple , effective tool for promoting the study of functional genomics and gene therapy .

水稻白叶枯病菌致病性功能基因组学分析广东水稻纹枯病菌RAPD基因型与致病型关系

Functional Genomic Analysis in Pathogenicity of Xanthomonas Oryzae Pv . oryzae ; Relationship between RAPD Genotype and Pathotype of Rice Sheathe Blight Fungi ( Rhizoctonia solani AG-1 IA ) in Guangdong Province

RNAi现广泛用于多个生物体的功能基因组学研究，以及在基因表达异常导致的多种疾病的临床前模型中进行干预治疗。

RNAi is currently used for both systematic functional genomics in several organisms and for specific therapeutic intervention in preclinical models of different diseases characterized by aberrant gene expression .

优化了S1核酸酶突变检测体系，新体系更加稳定、快速，适合于功能基因组学研究的要求；

S1 nuclease mutation detection system became more stable after the modification and the efficiency of detection was improved , which accorded with the requirements of functional genomics research .

对目前RNA介导的病毒抗性的特点、机制以及与转基因沉默的异同作一概括，并对RNA介导的病毒抗性在植物功能基因组学研究中的应用作了简单介绍。

This paper reviewed the RNA - mediated resistance including features , mechanism and similarities and differences with transgene - silence . Meanwhile , the application of RNA - mediated virus resistance to plant function genomes is sim-ply introduced .

本文综述了植物dsRNA介导的转基因沉默现象及其特点、分子作用机制、主要介导方法，以及近年来在植物功能基因组学研究上的应用情况。

In this review , we will discuss the principles , approaches and applications of the dsRNA-mediated gene silencing technique for plant gene functional analysis .

文章主要综述了RNA干涉（RNAi）技术在植物功能基因组学中的研究策略及研究现状，包括RNA干涉的作用机制及技术特征，引发植物RNAi机制的转基因RNAi技术以及病毒介导的基因沉默技术。

This review summarized the current progress and study strategy of RNA interference ( RNAi ) in plant functional genomics , including RNAi mechanism and characteristics , and progress on two technologies to produce RNA interference in plant .

同时，展望了VIGS技术作为植物功能基因组学功能分析的高通量技术平台的美好前景。

The review provides the prospects for VIGS as a powerful and high-through tool for assessing and characterizing the function in plant function genomics .

RNAi文库作为一种简单、高效、大规模、高通量的功能基因组学研究的工具，将在基因功能研究、发现新的药物靶基因、发现疾病相关基因等方面有广阔的应用前景。

As a simple , effective , large scale and high-throughput screening technique in functional genomics , RNAi library can be used in functional genetic screening , drug target discovery and validation , disease gene discovery and many other areas .

新型多价大肠杆菌噬菌体285P分离鉴定及功能基因组学研究

Isolation and Identification of a Novel Polyvalent E. Coli Phage 285P and Study of Functional Genomics

总之，RHDV反向遗传研究系统的建立将为深入研究其功能基因组学以及致病和变异的分子机制奠定基础，同时还具备研制新一代基因缺失疫苗和标记疫苗的潜在应用前景。

In a word , RHDV reverse genetics can be used to study functional genomics , molecular mechanism of pathogenesis and variation of RHDV and show some prospects in research of gene-deleted vaccine and marker vaccine .

本研究通过RT-PCR、Western-blot、组织微阵列、间接免疫荧光、以及免疫共沉淀等方法，从功能基因组学角度研究了CASK和annexinⅠ在食管鳞癌中的表达、定位和相互作用关系。

In the present study functional genomic analysis of annexin I and CASK proteins in the ESCC had been investigated via semi-quantitative RT-PCR , Western blot , immunohistochemistry staining ( IHC ) of the multi-tissue arrays ( MTAs ) and indirected immunofluorescence staining ( IIF ) techniques .

现对TILLING的技术流程、核心与特点，及其在突变研究、反向遗传学及功能基因组学、SNP检测、资源创新与分析以及作物遗传改良等方面的应用进行了综述。

The methodology , technical core and advantages of TILLING were introduced and its applications in mutation research , reverse genetics and functional genomics , detection of single nucleotide polymorphism ( SNP ), germplasm enhancement and evaluation , and crop genetic improvement were reviewed in this paper .