分子信标
- 名Molecular beacon;molecularr beacon
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基于分子信标实时监测大肠杆菌DNA连接酶催化的DNA连接过程
Real-time Visualization of Ligation Reaction Catalyzed by E.coli DNA Ligase Based on Molecular Beacon
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分子信标在DNA生物传感器中的应用
Application of Molecular Beacon in DNA Biosensors
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分子信标荧光探针用于遗传物质的超灵敏检测及超微型DNA、pH传感器的研究
Ultrasensitive Detection of Gene Expression Based on Molecular Beacons and Ultrasmall DNA pH Sensors
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分子信标用于不对称PCR检测乙型肝炎病毒
Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR
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基于分子信标的DNA计算
DNA Computing Based on Molecular Beacons
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基于分子信标的尿嘧啶DNA糖苷酶活性测定
Assaying Uracil-DNA Glycosylase Using Molecular Beacon
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基于分子信标探针的荧光定量PCR方法检测转基因食品
Fluorescence Quantitative PCR Detection of Transgenic Food Based on Molecular Beacon Probe
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建立了基于硫代修饰分子信标检测活细胞内mRNA表达的新方法。
MRNA detection in living cell using phosphorothioate-modified molecular beacon .
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改良分子信标-实时PCR快速检测产单核李斯特菌
Rapid simultaneous detection of Listeria monocytogenes using modified molecular beacons and real-time PCR
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分子信标荧光探针用于核酸连接过程的实时监测及病毒RNA的检测
Monitoring Nucleic Acids Ligation and Detecting Virus RNA Based on Molecular Beacons
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用于HBV病毒快速诊断的分子信标探针设计
Molecular Beacon Probes Design for Rapid Hepatitis B Virus Diagnosis
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将对虾白斑杆状病毒的一段特异性DNA设计成分子信标探针,用于该病毒的PCR检测。
A molecular beacon probe was used in the detection of prawn white spot bacilliform virus ( WSBV ) .
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应用分子信标荧光PCR定量检测乙型肝炎病毒核酸的评价
Evaluation of a Molecular Beacon Fluorescence PCR Kit for Quantitative Detection of Hepatitis B Virus Nucleic Acid
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以这种方式,每条单链DNA可以多次循环使多个分子信标被切开,从而实现信号放大。
In this way , each ssDNA can go through many cycles , resulting in cleavage of many beacons , achieving a signal amplification .
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Survivin基因mRNA分子信标定量检测方法建立及在肠癌中的应用
Quantification of mRNA expression of survivin gene by molecular beacon and its applications in colorectal colorectal carcinoma
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近年来,有关非放射性基因探针、DNA生物传感器探针及分子信标探针等技术研究取得的重要进展,必将加速基因探针技术在食品微生物检测中的应用。
Some important development about DNA probe technology has been obtained in recent years which will certainly accelerate its application in the detection of food pathogens .
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提出了一种基于分子信标和酶催化放大的电化学DNA传感器的研制方法。
We developed herein a novel electrochemical biosensor for quantification of DNA based on conformational change of molecular beacon caused by hybridization with subsequently enzymatic amplification quantification .
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副溶血性弧菌tdh基因的分子信标PCR技术检测
Detection of thermostable direct hemolysin gene in Vibrio parahaemolyticus by molecular beacon PCR
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PCR产物与结核分支杆菌分子信标杂交优化及荧光检测的初步实验研究
Investigation on the optimizing of the hybridization of PCR product with the molecular beacon of bacillus tuberculosis and the fluorescence detection of bacillus tuberculosis
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常规分子信标在活细胞内mRNA表达水平的研究中,由于细胞内核酸酶对分子信标骨架的降解和破坏作用,常导致假阳性信号的产生,对检测结果的准确性影响较大。
The normal molecular beacons tend to generate false positive signals due to nuclease degradation , when used for intracellular mRNA expression level analysis .
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基于分子信标和S1核酸酶发展了一种检测锌离子的新方法。
A new method for zinc detection based on molecular beacon and S1 nuclease was developed .
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目的建立分子信标定量检测Survivin基因mRNA表达的方法,对方法进行评估,并应用于临床。
Objective To develop and evaluate a real time fluorescent quantitative method for quantification of mRNA expression of survivin gene based on molecular beacon technique .
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本文首先介绍了DNA计算的基本思想,然后介绍发夹结构在解决SAT问题上的应用,再介绍发夹结构的一种特殊形式-分子信标的原理和设计方法。
Basic thinking of DNA computing is introduced in this paper , then the solution of SAT problem is introduced . After this , we introduce the principle and design of molecular beacons .
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此外,在这种稳定性较好的传感界面中,固定于电极表面的核酸分子信标也可作为一种捕获探针与单链DNA分子互补杂交,用于特定基因的分析检测。
In addition , the hairpin molecular beacon immobilized on the gold electrode surface can also act as a capture probe to hybridize with single-strand DNA , and it can be used for the specific gene analysis .
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同时,用Vero细胞和PC-3前列腺癌细胞作对照。吸附法制备分子信标壳聚糖纳米复合物。
PC-3 prostatic carcinoma cells and Vero cells were used as control.3.Molecular beacon nanoparticle complexes were prepared using incubation procedure .
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在解决这些问题的基础上,又总结了以往0-1规划问题的DNA计算,再结合分子信标的光学特性,提出光、电、机(计算机)一体的DNA计算模型;
On the basic of solving these NP complete problems , with optical characteristic of molecular beacons , I summarize the DNA computing of 0-1 integer programming problem , then bring forward a DNA computing module with light , electricity and computer .
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NASBA荧光分子信标技术定量检测丙型肝炎病毒
HCV Quantity Detection with NASBA Fluorescent Molecular Bacon Technique
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目的建立改良分子信标-多重实时PCR同时检测沙门菌和志贺菌的快速方法,应用于食源性致病菌的快速诊断。
Objective Dual detection of Salmonella and Shigella using modified molecular beacons and real-time PCR was developed . The established method was applied to rapid diagnosis of Salmonella and Shigella ' food poisoning , and for routine monitoring programs .
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使用分子信标作为S1核酸酶的底物,通过检测不同锌离子浓度下酶切初速度的变化,实现了对锌离子简便、快速的检测。
Molecular beacon was employed as the substrate . By monitoring the initial velocity change of S1 nuclease with different zinc concentrations , zinc detection could be achieved .
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文章采用分子信标编码方法,在解决SAT问题的同时解决MAX-SAT问题。
This paper encodes the candidate solution using molecular beacons . In this method , it solves the MAX-SAT problem while solving SAT problem .