免疫磁珠
- 网络MACs;imb;dynabeads
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与其他分选方法相比,免疫磁珠法具有分离效率高,细胞活性好,操作简便等优点。
MACS technology has many advantages over other cell sorting methods , such as high efficiency , high cell viability and easiness to operate .
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免疫磁珠细胞术分选脐血CD(34)~+细胞方法的改良
Improvement of sorting cord blood cd_ ( 34 ) ~ + cells by MACS
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免疫磁珠富集母体外周血胎儿DNA方法的研究
Study of Method to Enrich Fatal Dna in Maternal Plasma with Immunology Magnetic Beads
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免疫磁珠法在肾移植HLA快速分型中的应用
Application of Immunomagnetic Beads Technique for Tissue Typing in Renal Transplantation
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应用免疫磁珠法分离脐血CD(34)~+造血细胞
Immunomagnetic positive selection of cd_ ( 34 ) ~ + progenitor cells from human cord blood
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应用单克隆抗体-免疫磁珠分离系统分离纯化再生障碍性贫血骨髓CD(34)~+细胞
Separating and Purification Bone Marrow CD_ ( 34 ) ~ + Cells in Aplastic Anemia with Monoclone Antibody-immunomagnetic Beads System
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利用免疫磁珠技术可以获得高纯度的白血病细胞来源DC,达到了目前DC疫苗的质量控制标准[1]。
High purity DCs which meet the quality criteria for DC vaccines at present [ 1 ] can be obtained by immunomagnetic microbeads .
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于是,我们采用了免疫磁珠与RT-PCR联用的方法。
Thus , we used the immunomagnetic bead enrichment combined with single-cell RT-PCR in the detection process .
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基于IgY的免疫磁珠夹心ELISA法检测日本血吸虫循环抗原的研究
Detection of Circulating Antigen of Schistosoma Japonicum by Immunomagnetic Bead Sandwich ELISA Based on IgY
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羟基淀粉沉淀对免疫磁珠法分离脐血CD(34)~+细胞及造血祖细胞增殖功能的影响
Colony culture and purification of CD_ ( 34 ) ~ + hematopoietic stem / progenitor cells from cord blood by MACS and HES
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免疫磁珠法分选小鼠肝脏Kupffer细胞及其特性观察
Isolation and purification of mouse liver Kupffer cells by immunomagnetic bead separation
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分离骨髓单个核细胞,用免疫磁珠法富集CD34+细胞、CD4+(Th)细胞。
Bone marrow mononuclear cells ( BMMNC ) were isolated and CD34 + and CD4 + cells were enriched by magnetic beads respectively .
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方法:采用免疫磁珠阴性选择方法分离纯化人外周血T淋巴细胞;单向混合淋巴细胞反应(MLR)分析共刺激分子对T细胞的激发作用;
Methods : T lymphocyte was purified by magnetic beads negative selecting .
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基于量子点和免疫磁珠技术同步检测抗弓形虫IgG和IgM抗体的实验研究
An Experimental Study on the Synchronizing Detection of Anti-toxoplasma IgG and IgM Antibody Based on Quantum Dot and Immune Magnetic Bead Technique
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〔方法〕采用PCR法、免疫磁珠法和神奈川现象培养基三者结合使用。
〔 Methods 〕 PCR was used in combination with immunomagnetic separation and Kanagawa phenomenon culture medium to determine the isolation rate of TDH + V.p strains from environment .
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方法:采用美国NIH标准微量淋巴细胞毒技术和免疫磁珠法分离TB淋巴细胞。
Methods : T , B lymphocytes were isolated by American NIH standard lympho cytotoxic technique and magnetic beads method .
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目的用不同的鉴定方法对应用免疫磁珠技术分离的乳腺干细胞(separatedhumanmammarystemcell,SHMEC)进行生物学特点的鉴定。
Objective To identify the biological character of the separated human mammary stem cell ( SHMEC ) by magnetic cell sorting ( MACS ) with different methods .
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方法:运用免疫磁珠富集进行分离培养,并用PCR进行毒力基因分析。
[ WTHZ ] Methods : Escherichia coli O157 : H7 was isolated with immune magnetic essay from foods and PCR was performed to detect the toxic gene of the strains .
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用Percoll密度梯度及免疫磁珠法分离骨髓巨核细胞;
Percoll density gradient and immunomagnetic beads methods were used to purify megakaryocytes from bone marrow .
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ABO反定型免疫磁珠试剂的开发与研制基于量子点及免疫磁珠技术快速检测弓形虫抗体的实验研究
Explore and Study on ABO Immunomagnetic Particle for Reverse Grouping ; Detection of anti-Toxoplasma antibodies by using quantum dots and immunomagnetic beads
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采用HE染色和TUNEL染色观察凋亡情况并用流式细胞仪检测PBL-T的凋亡率和淋巴细胞亚群分布。结果采用免疫磁珠分离的PBL-T体外培养12h后,经诱导发生凋亡的细胞出现DNA断裂;
The apoptotic cell death of PBL-T was detected by TUNEL staining and assessed by flow cytometry .
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孕9天应用免疫磁珠分选孕鼠脾脏T、B细胞,并用CFSE体外荧光标记。
Purified T and B cells were isolated from spleen using magnetic beads at day 9 of gestation and were labeled with CFSE in vitro .
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免疫磁珠分离法分选提纯骨髓造血干细胞CD117
Isolation and Purification of Murine Hematopoietic Stem Cell CD117 with Magnetic Activated Cell Sorting Method
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方法应用免疫磁珠法(MACS)分选C57BL/6鼠CD4+CD25+T细胞。
Methods CD4 ~ + CD25 ~ + regulatory T cells were purified from C57BL / 6 mouse and using MACS .
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同时用免疫磁珠分离技术(IMS)直接检查隐性感染大鼠肠道上皮细胞内的Ty。
Meanwhile , to directly isolate parasitized Ty in the cecum epithelium of subclinical infected animals by using immunomagnetic separation method ( IMS ) .
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结论免疫磁珠法是分离脐血CD133阳性细胞的较好的方法。
Conclusions Magnetic cell sorting was a good method for separating CD133 + cell from cord blood .
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结晶紫染色法观察免疫磁珠分选的CML慢性期5例和5名正常人骨髓CD34+细胞与FN的粘附功能;
The adhesion function of immunomagnetic beads selected CD34 + cells to fibronectin was detected by crystal violet staining in 5 untreated CML-CP patients and 5 healthy donors .
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应用免疫磁珠分离成人骨髓中STRO-1阳性细胞
Isolation of STRO-1 positive cells in adult marrow by immunomagnetic selection
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结果:免疫磁珠分选Thy-1·1+细胞纯度达94·2%。
Results : The purity of Thy-1.1 ~ + cells after MACS was 94.2 % .
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方法采用免疫磁珠法测量转染WeelHu基因的NIT细胞培养上清中的胰岛素水平及观察其增殖速度。
Methods The level of insulin in culture supernatant of NIT cells was measured by immune magnetic bead method and their growth rate was observed .