激光解吸

基体溶液pH值对基体辅助激光解吸电离质谱法测量结果的影响

Effect of pH in Matrix Solution on Analytical Results of Matrix-assisted Laser Desorption Ionization Mass Spectrometry

以2-氨基-5-硝基噻唑为基质辅助激光解吸/电离飞行时间质谱的有效基质分析DNA及蛋白质样品

Analysis of Oligonucleotide and Protein via Matrix-assisted Laser Desorption / Ionization Time-of-flight Mass Spectrometry Using 2-Amino-5-nitrothiazole as an Effective Matrix

采用碱性新基质2-氨基-5-硝基噻唑对DNA和蛋白质样品进行了基质辅助激光解吸/电离飞行时间质谱分析。

Using 2-amino-5-nitrothiazole as a matrix , the matrix-assisted laser desorption / ionization time-of-flight mass spectrometric study of oligonucleotide and protein was carried out .

直接将细胞色素C吸附在硝酸纤维素（NC）膜上，进行了基质辅助激光解吸电离－飞行时间－质谱（MALDI－TOF－MS）分析。

Cytochrome C absorbed on nitrocellulose ( NC ) membrane was directly analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry ( MALDI TOF MS ) .

本文就表面增强激光解吸电离（Surfaceenhancedlaserdesorption/ionization，SELDI）蛋白质芯片的原理及近年来在肿瘤标志物筛选中的应用做一简要综述。

This article introduces the theory of surface enhanced laser desorption / ionization ( SELDI ) protein chip and its application in selecting of tumor markers .

近年发展起来的表面增强激光解吸/离子化-飞行时间-质谱（Surfaceenhancedlaserdesorption/ionization-TimeofFlight-massSpectrometry，SELDI-TOF-MS）技术是一种新的蛋白质组学研究方法。

Surface enhanced laser desorption / ionization-time of flight-mass spectrometry ( SELDI-TOF-MS ) is a new method of proteomics developed in recent years .

近年来开展的表面加强激光解吸电离-飞行时间质谱（Surfaceenhancedlaserdesorption/ionizationtime-of-flightMassSpectrometry，SELDI-TOF-MS）技术为这一探索提供了优良的技术平台。

A novel technology , surface enhanced laser desorption / ionization-time of flight-mass spectrometry ( SELDI-TOF-MS ) carved out such a new path to proteomics research on breast cancer .

随着基质辅助激光解吸电离质谱（MALDIMS）和电喷雾离子化质谱（ESIMS）两种技术的迅速发展，生物质谱技术已经成为生命科学研究中的重要工具。

With the rapid development of MALDI-MS and ESI-MS , biological mass spectrometry technology has already become an important tool in life science .

样品的制备与纯化是基体辅助激光解吸/电离飞行时间质谱（MALDITOFMS）能成功用于脱氧核糖核酸（DNA）分析的关键所在。

Sample preparation and purification are crucial in deoxyribonucleic acid ( DNA ) analysis by marix-assisted laser desorption / ionization-time of flight-mass spectrometry ( MALDI-TOF-MS ) .

应用基质辅助激光解吸电离飞行时间质谱技术研究了激光强度对酸性多肽（acidicpeptide，AP）解吸电离和稳定性的影响。

Effects of laser intensity on the desorption ionization and stability of acidic peptide ( AP ) were studied with matrix assisted laser desorption / ionization time of flight mass spectrometry .

选用基质辅助激光解吸离子化飞行时间质谱技术研究不同激光强度对猪血管紧张肽（AngiotensinⅠ，AnⅠ）解吸离子化的百分率和一级结构的稳定性。

The percentage of desorption ionization and the stability of primary structure of angiotensin ⅰ( An ⅰ) were studied using different laser intensities with matrix-assisted laser desorption / ionization time-of-flight mass spectrometry .

报道用基体辅助激光解吸电离质谱法（MALDI－MS）测定DNA分子的一种有效基体3－羟基－4－甲氧基肉桂酸。

A kind of effective matrix , 3 _ hydroxy _ 4 _ methoxycinnamic acid , in measurement of DNA by matrix assisted laser desorption ionization mass spectrometry ( MALDI-MA ) is reported .

目的运用双向电泳和基质辅助的激光解吸电离飞行时间质谱（matrix-assistedLaserDesorption/ionizationtime-of-flightMassSpectrometry，MALDI-TOF-MS）的方法，鉴定年龄相关性白内障和正常晶状体之间差异表达的蛋白质。

Objective To identify the different expressed proteins between the normal lens and age-related cataract by two-dimensional gel electrophoresis ( 2-DGE ) and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry ( MALDI-TOF-MS ) .

近年来表面增强激光解吸/离子化飞行时间质谱（SELDI-TOF-MS）技术已在许多肿瘤标志物研究中应用并取得成功。

Promising diagnostic patterns have recently been reported using surface enhanced laser desorption / ionization-time of flight-mass spectrometry technology ( SELDI-TOF-MS ) .

采用红外示差光谱、13C-核磁共振波谱和激光解吸质谱对玉米秸秆施入土壤后土壤胡敏酸（HA）的谱学特征的动态变化进行了研究。

Spectroscopy measurements ( Fourier transform infrared differential spectroscopy , Carbon-13 nuclear magnetic resonance spectrometry , Matrix-assisted laser desorption / ionization-time of flight mass spectrometry ) were performed to study the humic acids in the soil applied with corn stalk .

此外也有研究利用近几年新出现的表面增强激光解吸离子化-飞行时间质谱（SELDI-TOF-MS）的方法在胰液和血清中寻找胰腺癌的标志物。

Besides , surface-enhanced laser desorption ionization ( SELDI ) approach had been used to search for biomarkers in serum and pancreatic juice .

利用基质辅助激光解吸飞行时间（MALDITOF）质谱技术，测定羧肽酶Y消化蛋白质和多肽。

The carboxypeptidase digestions of peptides were analysed for C terminal sequences by matrix assisted laser desorption / ionization time of flight ( MALDI TOF ) mass spectrometry .

从凝胶上切下差异蛋白质点，通过基质辅助激光解吸/离子化飞行时间质谱（MALDI-TOF-MS）鉴定。

Then the protein spots were excised from gels and identified by the matrix-assisted laser desorption / ionization time of flying mass spectrometry ( MALDI-TOF-MS ) .

利用激光解吸飞行时间质谱技术分析了目标产物中半乳糖、5-FU与HSA的结合状态、糖密度（半乳糖摩尔数/蛋白摩尔数）和药密度（药物摩尔数/蛋白摩尔数）；

The MALDI-TOF-MS ( Matrix assisted laser desorption ionization time of flight mass spectrometry ) was applied to analyze the Gal-HSA-5-FU for its molar ratio of the galactose to HSA and the 5-FU to HSA .

基质辅助激光解吸电离（MALDI）技术是近年来发展起来的新的质谱离子化技术。

Matrix assisted laser desorption ionization ( MALDI ) technology is one of the best mass spectrometry ionization technologies for macro molecules which was developed almost two decades ago .

目的用表面增强激光解吸离子化飞行时间质谱仪（SELDI-TOF-MS）检测慢性移植物肾病（CAN）患者血清蛋白质图谱，构建CAN血清蛋白质指纹图谱及其对该病的早期诊断作用。

Objective To construct protein fingerprints in serum and to early diagnose chronic allograft nephropathy ( CAN ) using surface enhanced laser ionization / desorption-time of flight-mass spectrometry ( SELDI-TOF-MS ) .

方法应用表面增强激光解吸离子化飞行时间质谱技术（SELDI）检测马尔尼菲青霉菌酵母相和霉菌相的蛋白质谱。

Methods Surface enhanced laser desorption / ionization ( SELDI ) time-of-flight mass spectra were performed to compare the expressed proteins between yeast form and mould form of Penicillium marneffei .

选用基质辅助激光解吸电离化飞行时间（MALDI-TOF）质谱技术分析茶多酚及茶多酚-锗的组成和结构。

Matrix-assisted laser desorption ionization / time of flight ( MALDI-TOF ) mass spectrometry was selected to analyze the composition , structure and stability of tea polyphenol ( TP ) and TP-Ge4 + .

利用海牛卵制备的模拟微量混合内切酶体系，用基质辅助激光解吸电离-飞行时间（MALDI-TOF）质谱技术研究了胰岛素在不同酸碱度和还原条件下的水解和酶解过程。

Using simulated micro mixed endopeptidase system prepared with crushed sea slug eggs , the hydrolysis and enzymolysis of insulin under different pH and reductive conditions were studied by MALDI TOF mass spectrometry .

目的表面增强激光解吸电离质谱（SELDI-TOF-MS）技术结合激光捕获微切割（LCM）分析正常结肠粘膜上皮细胞和结肠癌细胞的蛋白质组学构成。

Objective To apply the surface-enhanced laser desorption / ionization ( SELDI ) technique coupled with laser capture microdissected ( LCM ) technique to analyse proteomics of human colorectal cancer cells and normal mucosa cells effectively .

本文以邻&硝基苯辛醚（NPOE）为液相基体，纤维纸为基底，用基体辅助激光解吸／电离飞行时间质谱法（MALDI－TOF－MS）测定紫杉烷类化合物，研究其激光裂解规律。

By using MAI , DI-MS with 2-Nitrophenyl octyl ether ( NPOE ) as liquid matrix and fibrous paper as substrate , four taxane compounds were measured . The fragmentation of their MALDI mass spectra was discussed .

选取差异蛋白质点，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）及蛋白质数据库检索进行差异蛋白质鉴定。

The differential proteins in the gel were selected and dug in situ to be identified by matrix-assisted laser desorption ionization time of flight Time mass spectrometry ( MALDI-TOF-MS ) and protein database searching . 2 .

将凝胶中的差异蛋白点切出，经胶内胰蛋白酶解和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析获得肽质量指纹谱。

The differential protein spots in the gels were cut , then the peptide mass fingerprint were obtained through In-gel trypsin digestion and matrix-assisted laser desorption / ionization time of flight mass spectrometry ( MALDI-TOF - MS ) analysis . 5 .

目的：探讨用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）技术筛查肺癌血清特异性蛋白质的临床意义。

Objective : To detect the serum proteomic patterns in lung cancer by surface enhanced laser desorption / ionization-time of flight-mass spectrometry ( SELDI-TOF-MS ) protein chip array techniques , screen biomarkers of lung cancer and build diagnostic models in order to evaluate their clinical significance .

选取差异表达蛋白质点用基质辅助激光解吸飞行时间质谱测定肽质量指纹谱，检索Swiss-Prot蛋白质数据库鉴定差异蛋白。

Differential expression protein was selected with matrix assisted laser desorption / ionization time of flight mass spectrometry ( MALDI / TOF MS ) so as to measure peptide mass fingerprinting . Swiss-Prot protein database was retrieved to identify differential expression protein .