液体培养

通过液体培养产毒实验，从现有的21个链格孢菌菌株中筛选出产毒最强的菌株NEW。

The highest-yield toxin strain NEW was screened from 21 owned strains by toxin liquid culture method .

本文以人白血病细胞株K（562）为实验模型，通过体外液体培养，研究了白血病细胞诱导剂的作用。

The effect of inducers on leukemia cell line K562 was studied in liquid culture system in vitro .

用硫酸铵沉淀法从液体培养物中纯化噬菌体λDNA

Purification by Ammonium Sulfate Precipitation of Bacteriophage λ DNA from LB-Broth Culture

方法液体培养法建立细胞系，用R显带和逆转录聚合酶链反应方法检测其遗传学标志；瑞氏染色、超微结构及组织化学染色观察细胞形态；

Method The cell line was established by liquid cell culture .

鉴别B群链球菌的液体培养基pH改变试验及其影响因素

Medium pH shift Test for Identification of Group B Streptococci and its Influencing Factors

液体培养基中加入的Fe对杀菌有促进作用。

Fe added into bacteria medium can promote the disinfection effect .

HPLC法测定蜜环菌液体培养菌丝的腺苷含量

HPLC for Rapid Determination of Adenosine in Liquid Fermentation Mycelium of Armillaria Mella

mL是液体培养刺激CD34+细胞体外增殖的最佳浓度。

ML of the preparation is the best consistence to liquid culture stimulate CD 34 + cells proliferation .

论文取得的结果主要如下：7株菌在TCP无机磷液体培养基中培养时，所有菌株的pH在3.88-5.18之间。

The main results of this study were as follows : The pH of seven strains droped 3.88-5.18 in the TCP liquid medium .

平菇液体培养基和普通营养肉汤培养基同时对80份分泌物标本培养，其检出率分别为38.8%和36.3%（p＞0.05）。

80 case of secreta were cultured on mushroom culture solution and general broth culture , the positive rate being respectively 38.8 % and 36.3 % ( p > 0.05 ) .

以叶用莴苣微茎尖为试材，在含有BA和NAA的MS液体培养基上旋转培养，诱导出大量愈伤组织；

Primordium-like tissue was established from the apical meristem under rotation culture on MS medium with BA and NAA .

在液体培养条件下探讨了培养基的碳源、氮源、初始pH及其正交试验对灰树花菌丝生长和产糖的影响。适合的碳源是葡萄糖+土豆，淀粉，葡萄糖+蔗糖；

The effect of carbon source , nitrogen source , initial pH and orthogonal test on the mycelial growth and polysaccharide production of Grifola frondose was investigated .

白金针菇的液体培养及6-BA对其菌丝体生长的影响

The liquid cultivation of flammulina velutipes and the effect of 6-BA on mycelium growth

愈伤组织在不含激素的MS固体培养基上可一步分化成完整小植株，在液体培养基中震荡培养可形成大量小块茎。

The callus could differentiate into the plantlets on solid culture and into plenty of tubers on liquid shake culture on MS mediums without plant growth hormone .

用RTPCR技术检测液体培养自发性生长细胞的Tpo的自主分泌和旁分泌；

The autocrine and paracrine of Tpo in the spontaneous growth cells in liquid culture were examined by RT PCR .

将胀果甘草的根、胚轴、子叶分别接种到含有不同激素组合的MS培养基上，在光照或黑暗下培养.将根接种到液体培养基中培养。

Root , hypocotyl and cotyledon of Glycyrrhiza inflata were cultured on MS medium containing different hormones in light or dark , and root was cultured in liquid medium .

毛状根能在无外源生长调节剂的MS固体和液体培养基上自主生长，但在液体培养基中培养的毛状根生长更迅速，也不会形成愈伤组织。

Hairy roots could have an autonomous growth on solid or liquid growth regulator-free MS medium but grew more rapidly and formed no callus during culture in liquid medium .

目的使用液体培养基，建立一种分枝杆菌（Mycobacterium）菌种初步鉴定的非放射快速荧光检测法。

Objective To set up a method of Rapid Fluorimetry for identification of group of Mycobacterium by using the liquid culture medium .

以虫荧光素酶法检验了四株丝状真菌在葡萄糖&无机盐液体培养过程中的胞内ATP含量。

The intracellular ATP contents in 4 strains of mycelial fungi , grown on the glucose-inorganic salts medium , were determined by luciferin-luciferase system .

其中，羊肚菌子实体干粉和由PDA液体培养基发酵产生的菌丝体冻干产物均不产生孢子。

Among them , morel fruiting body powder and freeze-dried product of PDA liquid medium fermentation of the mycelium did not produce spores .

该两株菌在苯胺选择性液体培养基中48h内对苯胺的降解率均在86%以上；在模拟实验中，48h内对土壤中苯胺的降解率分别达到93.4%和96.6%。

Degradation rate of the two strains were all above 86 % after 48h in liquid aniline selected medium .

黄萎病菌液体培养的粗毒素经凝胶柱层析，得到2个有紫外吸收（OD（280））的组分（P1和P2）。

The raw toxin extracted from the liquid culture of pathogens was fractioned through a gel column chromatography and 5 fractions .

细菌检测采用平板划线法、平板涂布法、液体培养和煮沸法快速提取DNAPCR扩增，通过人为控制，每种方法都能检测到一个最低限度。

Bacteria are detected by means of streak plate , plate coating , and boiling as well as liquid culture for DNA PCR extraction . A limit can be reached with each method by manipulation .

A7琼脂固体和液体培养联合应用检测泌尿生殖道解脲脲原体

Application of A7 Agar Combining with Broth Culture in Detection Ureaplasma Urealyticum in Urogenital Specimens

结果表明，A1、A10两个烟草赤星病菌菌株的孢子萌发液和液体培养滤液中都含有毒性代谢物，这种毒性代谢物具有致病性和寄主专化性，因此，它是病原菌的关键致病因子。

The results showed that in spores germinating solution and liquid culture filter of Aland A10 , there were toxic metabolite .

结果130份标本中，液体培养法Uu阳性71份、判为污染的13份、阴性46份。

Results Among the 130 specimens , there were 71 Uu positive , 13 polluted and 46 negative results in broth method ;

将液体培养物稀释到同一梯度，定量接种于固体培养基上（绵羊血琼脂平板和特殊培养基），分别进行需氧和厌氧培养24~48h。

Things of the above cultured , after diluted to the same gradient and inoculated on solid culture mediums quantitatively , were cultured for 24 ~ 48 h under the condition with oxygen and without oxygen .

本研究为来自11例AML的骨髓或血的单个核细胞的液体培养研究。

In this study bone marrow and / or blood mononuclear cells from 11 patients with AML were observed in liquid suspension cultures .

采集男性精液或女性分泌物接种于UU液体培养基中培养观察结果。

To collect the male patient 's semen or female patient 's secreta inoculating with UU liquid culture medium and observe its results .

在液体培养中，病菌对氮源的利用以酵母膏为最好，其次为（NH4）2SO4。

Yeast extract WBS the best nitrogen source in liquid culturate , the second was ( NH4 ) 2SO4 .