杂交瘤细胞

EBV转化的人B淋巴母细胞和人B淋巴细胞杂交瘤细胞表面标记表达的研究

Analysis of The Expression of Surface Markers on Human LCLs and Human B-Lymphocyte Hybridoma Cell Lines

无牛血清IgG细胞培养基（-GFCS培养基）的制备及其在杂交瘤细胞体外培养中的应用

Preparation and application of-GFCS medium to culturing hybridoma cells in vitro

γ辐射对B淋巴杂交瘤细胞抗体分泌功能作用研究

Studies of antibody secreting functions of B lymphocyte hybridoma cells after γ - irradiation

分泌抗HLAClassⅠ单态性单克隆抗体杂交瘤细胞的建株和鉴定

Establishment and identification of anti-HLA class I monomorphic monoclonal antibody-secreting hybridoma cell line

8－氮鸟嘌呤诱变3D5杂交瘤细胞的初步研究

The primary study of mutagenesis of hybridoma 3D 5 against human erythrocyte with 8 azaguanine

通过染色体组型分析，确认AL01和AL02皆为杂交瘤细胞。

By genome analysis , AL-01 and AL-02 were proved to be hybrid cells .

白细胞介素6诱导小鼠T细胞杂交瘤细胞c－fos基因表达

C-fos Gene Expression Induced by IL-6 in the Mouse T Cell Hybridoma

抗链霉素杂交瘤细胞株的建立与竞争ELISA试剂盒的研制

Filtration of hybridoma lines and development of rapid test competitive ELISA kit for streptomycin

用相应抗原包被，进行间接Elisa筛选得到阳性杂交瘤细胞。

Coated with the corresponding antigen , then screened positive hybridoma through indirect Elisa .

分泌抗LDH-C4pIgA和IgG抗体的杂交瘤细胞在生殖道的归巢

Homing of hybridoma cells secreting pIgA and IgG against LDH-C4 to genital tracts

经间接ELISA和间接免疫荧光试验筛选，共获得了15株NDV杂交瘤细胞。

After screening by indirect ELISA and IFA , 15 MAbs were obtained .

获得3株稳定分泌IgM型天然抗角蛋白单克隆抗体的杂交瘤细胞株。

Three hybridoma strains secreting natural IgM monoclonal antibody against keratin were obtained .

抗人IgM单克隆抗体的研究&脾内免疫建立抗人IgM单克隆抗体杂交瘤细胞株

Studies on Monoclonal Antibody against Human IgM & Establishment of McAb Hybridoma cell

用纯化的蛋白包被ELISA反应板，对融合成功的杂交瘤细胞进行筛选。

The ELISA plates were coated with purified protein and hybridoma culture supernatants were screened by indirect ELISA method .

用建立间接ELISA方法检测杂交瘤细胞上清，结果阳性率为20.6%。

The supernatant of hybridoma cells were detected by indirect ELISA , and the positive ratio was 20.6 % .

采用间接ELISA试验和HI试验双向检测杂交瘤细胞分泌的抗体，阳性率为78.4%；

Our screening of the hybridoma cells by indirect ELISA and HI , the positive rate is 78.4 % ;

植物抗体基因工程：I.分泌马铃薯Y病毒中和抗体杂交瘤细胞系的筛选

Antibody Genetic Engineering in Plant : I.Selection of a Hybridoma Cell Line Secreting Monoclonal Antibody Against Potato Virus Y

Vero、CHO和杂交瘤细胞在生物反应器中的生长和代谢

Physiology of Vero , CHO and Hybridoma Cells Cultured in Bioreactor

信号转导抑制剂对囊素刺激杂交瘤细胞抗体分泌及其mRNA表达的影响

Influences of signal transduction inhibitors on the production of monoclonal antibody and the expression of γ _1 mRNA in hybridoma cells stimulated by bursin

脾细胞通过传统PEG法与骨髓瘤细胞SP2/0融合制备杂交瘤细胞。

Spleen cell was fused with myeloma cells SP2 / 0 through the traditional PEG method to prepare hybridoma .

将上述3株杂交瘤细胞分别注入BalbC小鼠腹腔，获得含抗赭曲霉素A单克隆抗体的腹水。

The ascites containing monoclonal antibodies against Ochratoxin A was gained via inoculation of the hybridoma cell into abdominal cavity of Balb / c mice .

将获得的阳性杂交瘤细胞株注入小鼠腹腔，制备腹水，ELISA检测其腹水效价均达到1.0×105。

The hybridoma cells were injected into mice abdominal cavity to prepare ascites . The titers of ascites were 1.0 × 105 by ELISA .

以此重组NP作为抗原制备抗NP单克隆抗体，获得两株持续稳定分泌单克隆抗体的杂交瘤细胞株。

With the rNP as antigen , two hybridomas secreting anti NP monoclonal antibodies persistently and stably were obtained .

单克隆抗体技术分泌抗细粒棘球蚴囊液抗原McAb杂交瘤细胞株的建立

Establishment of hybridoma cell line secreting monoclonal antibodies ( McAb ) against hydatid cyst fluid antigen

抗人IgG单克隆抗体的研究&Ⅰ.分泌抗人IgG单克隆抗体杂交瘤细胞株的建立

Studies on the monoclonal antibodies against human IgG I. The establishment of the hybridoma cell lines secreting monoclonal antibodies against human IgG

应用细胞融合技术建立抗人IgG4McAb杂交瘤细胞，生产McAb。

Hybridomas producing monoclonal antibodies ( McAb ) against human IgG4 were prepared by cell fusion technique .

获得的杂交瘤细胞直接经IFA筛选。

The hybridomas were detected by IFA directly .

方法用RT-PCR方法从能分泌特异性抗人AFP单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。

Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma .

结果获得了1株稳定分泌抗人晶状体上皮细胞McAb的杂交瘤细胞系，其亚类为IgM。

Results One hybridoma cell line secreting anti-human lens epithelial cell McAb was successfully obtained , its subclass is IgM .

用间接ELISA和有限稀释法对可产生抗SEI抗体的杂交瘤细胞进行筛选和克隆扩增。

The hybridoma cells capable of producing antibodies against recombinant SEI were screened by indirect ELISA and cloned by limiting dilution .