感受态

通过DNA释放及感受态建立进行的枯草杆菌细胞间自然转化

Bacillus subtilis Undergo Cell-to-Cell Natural Transformation via DNA Releasing and Competence Acquiring

聚-β-羟基丁酸（PHB）在细菌建立感受态中的作用

The Function of Poly - β - Hydroxybutyrate ( PHB ) in the Formation of Competence in Bacteria

细菌感受态细胞摄取和分泌DNA的相关性研究I.含有质粒的枯草芽孢杆菌自然感受态缺陷突变株的诱变和分离

Study of the Interrelations Between DNA Uptake and Secreting of Competent Bacterial Cells I Mutagenesis and Isolation of Natural Competence-deficient Mutant from Containing Plasmid Bacillus subtilis

通过转化感受态大肠杆菌，挑选阳性克隆，提取重组质粒，并对抽提的质粒进行限制性酶切反应，DNA测序鉴定。

Recombinant vectors were then transformed to E-coli , The positive colony were selected and recombinant plasmids were extracted . After restricted enzyme digestion and sequencing , the plasmids were identified correctly .

对感受态细胞的存储时间及其与转化效率之间的关系也进行了研究，结果显示感受态细胞可以在-20℃存储7d，在-70℃存储15d。

The storage time of competent cells and its correlation to transformation efficiency were studied .

制备肺炎链球菌感受态，首先在不同菌密度下转化外源DNA，计数抗生素筛选平板上的转化菌落，比较其转化率，确定转化的最适菌密度；

First exotic DNA was transformed under different cell density then the transformed colonies on antibiotic screening plate was counted to compare their transformation rate to ascertain the most suitable cell density for the transformation .

结果：野生菌株的毒力基因受感受态刺激因子（CSP）诱导表达增加；

RESULTS : The mRNA expressions of the five virulence genes in wild strains were induced by competence-stimulating peptide ( CSP ) .

两种连接产物分别转化E.ColiDH5α感受态细胞，提取质粒DNA后，对重组质粒进行PCR和酶切鉴定。

Two connection products were transformed into E.coli DH5 α competent cells and recombinant plasmids were identified by PCR and enzyme digestion after plasmid DNA were extracted .

二者连接后，转化入DH5α感受态细胞（大肠杆菌）中。用蓝白斑筛选法识别PCR产物是否与pUC19载体连接。

After ligation , they were transformed into DH5 α competent cells ( Escherichia coli . )

然后在此菌密度下比较CSP诱导不同时相的转化率，同时用RT-PCR检测感受态调控基因comE的表达。

Under this cell density compare the transformation rate at different time and induced by CSP-2.And at the same time , the expression of regulator and control gene comE of competence was tested with RT-PCR .

基因comE、comX、cinA、dnaK在肺炎链球菌感受态形成中作用的研究

Research the Function of Gene comE 、 comX 、 cinA 、 dnaK in Competence of Streptococcus Pneumoniae

将重组质粒电转化毕赤酵母GS115感受态细胞，甲醇诱导蛋白质表达。

The recombinants were induced by methanol for the expression of recombinant protein .

扩增其核苷酸序列并克隆至pET-32a（+）载体中，将阳性重组质粒pET-32-S1转化感受态细胞BL21（DE3）。

The nucleotide sequence was amplified and cloned into pET-32a ( + ) vector to generate recombinant plasmid pET-32-S1 .

Voytas说该技术只需要标准分子生物学实验室和引入DNA至植物的感受态，这些资源在很多发展中国家都可获得。

Voytas says the technique requires only standard molecular biology laboratories and competence in introducing DNA into plants & resources that are available in many parts of the developing world .

经PCR、双酶切及测序鉴定后将重组质粒PET-32a（+）-gI转化入大肠杆菌BL21（DE3）感受态细胞进行表达。

After being confirmed by PCR , restriction endonuclease digestion and sequencing , pET-32a ( + ) - gI was transformed into E.coli BL21 ( DE3 ) competent cells for overexpression .

将该重组质粒转化大肠杆菌BL21（DE3）感受态细胞，经诱导获得大小约173kDa的表达蛋白（rPMT）。

Coli BL21 ( DE3 ) competent cells . After induction by IPTG , a 173 kDa recombinant protein ( named rPMT ) was expressed .

本论文研究了野油菜黄单胞菌（Xanthomonascampestris）菌种的不同生长状态、不同转化液等因素对感受态细胞转化率的影响。

The effects of the different growth status , the different transformation liquid and other factors on the transformation rate of the recipient cell of Xanthomonas campestris was studied in this paper .

肺炎克雷伯菌对17种抗生素均耐药，感受态大肠杆菌对17种抗生素均敏感，转化子DH5α对头孢呋辛和氨苄西林耐药，对其余15种抗生素表现不同程度的敏感。

Klebsiella pneumoniae was resistant to 17 antibiotics and Bacillus coli was sensitive to all antibiotics , DH5 α was resistant to cefuroxime and ampicillin , but sensitive to others .

成功构建了原核表达载体pET-30b-IGFBP-6和pET-30b-IGFBP-6-C，转化E.coli感受态细胞，筛选到了高表达量的菌株，优化了表达条件。

Constructed the prokaryotic expression vector pET-30b-IGFBP-6 and pET-30b - IGFBP-6-C , transformed the E.coli competent cells , screened the high level production strain , optimized the expression condition . 2 .

每种菌株的感受态细胞制备的最佳光密度（OD600值）不同。

The optimal optical density ( OD_ 600 ) range for competent cell preparation varied for each of the strains .

方法采用PCR技术从金黄色葡萄球菌染色体DNA上扩增得到SEB基因，克隆到原核表达载体pET22b（+）上，转化大肠杆菌BL21（DE3）感受态细胞，全自动测序仪测序。

Methods The full-length of SEB gene amplified from DNA of S6 strain of Staphylococcus aureus by high-fidelity PCR was cloned into prokaryotic expression vector pET22b ~ + , and then the vector was transformed into BL21 ( DE3 ) to construct a prokaryotic expression system .

报道表明，造成转化效率过低的原因之一是由于较低的感受态水平。枯草芽孢杆菌感受态的形成是在T0时期，即菌体生长的对数生长期到稳定期。

One of low efficiency reasons has been reported which was due to a much reduced level of competence , Competence in Bacillus subtilis develops after To , which is the point of transition from exponential growth to stationary phase .

用EcoRⅠ和SalⅠ对目的基因片段和载体pPICZαA进行双酶切，酶切产物经连接后导入大肠杆菌DH5α感受态细胞中。

After the gene fragments of S1-S6 and pPICZ α A were digested by EcoR I and Sal I , these two digested fragments were connected in common ways and transformed into the E. coli DH5 α competent cells .

从麦洼牦牛脊髓中提取总RNA，利用RT-PCR技术扩增血红蛋白β链基因，获得了513bp的cDNA，连接到PMD-18载体，进而转化到TOP-10感受态细胞中进行克隆测序。

Total RNA was extracted from the spinal cord of Maiwa yak , and RT-PCR was carried out to amplify the gene of hemoglobin β chain . The amplified 513 bp cDNA was ligated to PMD-18 vector , transformed to TOP-10 competent E. coli cells and sequenced .

以大肠杆菌DH5α为受体菌，研究CaCl2溶液浓度、感受态细胞的存放时间、转化时间及转化后的培养时间等对质粒转化效率的影响。

The different effects of the transformative condition which included CaCl_2 concentration , of the competent cells status , of the various transformative time and of the incubative time on the transformative plasmid were investigated . The host cell was Escherichia coli DH5 α .

温度对大肠杆菌感受态细胞的影响

The effect of temperature on the competent cell of Escherichia coli

结果发现不同肺炎链球菌株的转化最适菌密度不同，构建了肺炎链球菌感受态缺陷菌株，建立了肺炎链球菌在实验室的转化模型。

Results Transformation model of S.pneumoniae in laboratory was established .

短小芽孢杆菌的遗传转化&Ⅰ.感受态菌株的筛选

Genetic transformation in Bacillus pumilus ⅰ . selection of the competent strain

肺炎链球菌感受态缺陷影响细菌对β-内酰胺类抗生素的敏感性

Streptococcus pneumoniae with competence-deficient changed its β - lactam resistance

根癌农杆菌感受态细胞的制备及保存方法研究

Study on Preparation and Preservation Method of Competent Cell of Agrobacterium Tumefaciens