单倍体化

目的：探索体细胞移入未去核成熟卵母细胞后单倍体化的能力。

Objective : To investigate the haploidization of somatic cell nuclei in non-enucleated mature oocytes regarding chromosomes and developmental potential .

研究发现单倍体的甲基化水平高于对应的二倍体，是单倍体相对于二倍体甲基化模式经过重新调整，在其基因组中甲基化水平发生了总体变化以适应生存的必然结果。

It showed that the general methylation levels of haploids was higher than that of their diploid partners , which were the outcomes of methylation regulation on genome level to adapt the haploidization .

单倍体的自然加倍率较高，无需人工加倍即可获得大量的双单倍体及多倍体，多倍体比例为33.16%，双单倍体化植株比例为51.34%。

Haploid doubling rate of high natural , no artificial double to get a lot of double haploid and polyploid polyploid ratio of 33.16 % , the ratio of double-haploid plants of 51.34 % . 9 .