分级沉淀

以鲨鱼软骨为原料，经盐酸胍抽提，丙酮分级沉淀，超滤等步骤得到鲨鱼软骨制剂（sharkcartilagepreparation，SCP）。

Shark cartilage preparation ( SCP ) was extracted from shark cartilage by the method of guanidine hydrochloride extraction , acetone fractional precipitation , and ultrafiltration .

以胸腔渗出液为来源，用硫酸铵分级沉淀，DEAE-cellulose离子交换柱层析和快速蛋白质液相分离仪纯化了C&反应性蛋白。

We purified C-reactive protein ( CRP ) from pleural exudate by using ammonium sulfate fractional precipitation , DEAE-Cellulose chromatography and fast protein liquid chromatography ( FPLC ) .

同时用硫酸铵分级沉淀发酵液中的蛋白，SDS-PAGE法分析有活性的蛋白组分。

Protein in fermented broth was precipitated with ammonium sulfate and the active part was assayed by SDS-PAGE .

离心发酵液以收集菌体，用缓冲液悬浮菌体，经过超声破碎、硫酸铵分级沉淀和Ni-NTA亲和层析，得到了电泳纯的Hyp。

Hyp was purified after cell collection , suspension , ammonium sulfate precipitation and Ni-NTA column chromatography .

还调整了第二次硫酸铵分级沉淀时的饱和度，并将38-45%饱和度下的沉淀物确定为需要收集的cytb6f制剂。

Secondly , the second ammonium sulfate fractionations was changed and the precipitates formed from 38-45 % were determined to be the Cyt b_6f preparation .

粗提物经丙酮分级沉淀及高压液相色谱分离，得到较纯的LP，经质谱仪测定分子量为803.6D。

An antibacterial peptide LP was purified by acetone precipitation and reversed phase column chromatography . The molecular weight of LP is 803.6 D determined by mass spectrometry .

进一步经过硫酸铵分级沉淀，DEAE-sepharoseFastFlow离子交换层析，Sephacryls-200凝胶过滤，获得电泳纯的杀虫蛋白。

Insecticidal protein from Serratia marcescens HR-3 was purified by ammonium sulfate precipitation , DEAE-Sepharose Fast Flow , Sephacryl S-200 gel filtration and native SDS-PAGE cuting method .

采用硫酸胺分级沉淀法、葡聚糖凝胶Sephadex-25层析脱盐、DEAE-纤维素纯化等方法获得绵羊血清免疫球蛋白G。

Sheep serum Immunoglobulin G ( IgG ) was purified by ammonium sulfate precipitation , Sephadex-25 chromatography and DEAE-Sepharose column chromatography , respectively .

用硫酸铵分级沉淀，DEAE-纤维素及DEAE-Sephadex柱层析的方法，可以从牛脑中提取烯醇化酶同工酶。SDS-聚丙烯酰胺凝胶电泳图谱显示牛烯醇化酶3在还原前后的分子量都是55000。

Enolase isozymes were isolated from bovine brain by ammonium sulfate fractionation , DEAE-cellulose and DEAE-Sephadex column chromatography .

通过硫酸铵分级沉淀（40%~80%）、凝胶层析（SephadexG-75）对米胚芽水溶物进行分离纯化。

Rice germ aquous solute was separated and purified through ammonium sulfate fractionation ( 0.4 ~ 0.8 saturation ) and gel chromatography ( sephadex G-75 ) .

黑曲霉（Aspergillusniger）319发酵液经硫酸铵分级沉淀、DEAE-纤维素52离子交换层析和SephadexG-100分子筛层析，得到了电泳纯的菊糖酶组分。

The main component of inulinase was purified from fermentation broth of Aspergillus niger 319 to homogeneity by using ammonium sulfate fraction , ion-exchange chromatography on DEAE-cellulose column and Sephadex G-100 gel filtration .

本研究的ApoAⅠ的表达为分泌表达，蛋白纯度高，占可溶性蛋白的70%以上，分离纯化简便，蔡钦生同学经低温丙酮分级沉淀一次性获得纯度高于90%的ApoAⅠ。

Because of secret expression with high level the ApoA ⅰ which accounted for 70 % in the soluble protein was very easy to be purified .

通过对热变性法、等电点法、丙酮分级沉淀法和硫酸铵盐析法等几种方法的实验分析比较，找出一套操作简便SOD总收率高的分离纯化工艺。

By comparing the experimental results obtained by some different ways of treatment , such as heat treatment , isoelectric fractionation , acetone fractionation and ( NH4 ) 2SO4 salting out , a new and simple process was found to achieve the high SOD recovery with specific activity .

采用硫酸铵（NH4）2SO4分级沉淀法提取几丁质酶液，得到提取木霉几丁质酶的最佳（NH4）2SO4浓度为75%。

The methord of ammonium sulfate grading precipitation was used to extract the chitinase solution . The best concentration of ( NH4 ) 2SO4 , which was used to extract the Trichoderma chitinase , was 75 % .

麻疯树核糖体失活蛋白（curcin）经匀浆、浸取、硫酸铵分级沉淀得粗品，将粗品于SephadexG-100分子筛层析得纯品，经SDS-PAGE检测为单一蛋白带。

Curcin was isolated from the seeds of JATROPHA CURCAS by extraction , precipitation with ( NH_4 ) _2SO_4 , gel filtration on Sephadex G-100 . The purified curcin showed a single protein band on SDS-PAGE .

几丁质酶通过（NH4）2SO4分级沉淀、阴离子交换柱层析、SDS-PAGE提纯后可以达到层析纯，出现单一并且峰值很高的层析峰，电泳确认两条带，分别为几丁质外切酶和几丁质内切酶。

Chromatographically pure chitinase could be obtained by ( NH4 ) 2SO4 fractional precipitation , anion-exchange chromatography , SDS-PAGE , and a singular and high chromatography peak could be found and two electrophoretic bands could be validated from gel electrophoresis .

通过硫酸铵分级沉淀、CM52阳离子交换层析、蓝胶亲和层析和FPLCMonoS阳离子交换层析，从丝瓜籽抽提液中分离到一种多肽LuffinP1。

A peptide , luffin P1 , from seeds of Luffa cylindrica , was purified by ammonia sulfate precipitation , CM-52 ion exchange chromatography , Blue-gel affinity chromatography and FPLC Mono S ion exchange chromatography .

大豆异黄酮糖苷水解酶经硫酸铵分级沉淀、DEAE-Cellocuse（DE-52）离子交换凝胶层析对发酵液进行了分离和纯化，并得到了电泳纯的酶。该酶被纯化了11倍，收率为10.9%；

The enzyme solution is purified by 11 folds and a yield of 10.9 % by ( NH_4 ) _2SO_4 precipitation and DEAE-cellulose DE-52 ion exchange chromatography .

经40%饱和度硫酸铵分级沉淀，SephadexG-200凝胶过滤等步骤，将棉铃虫血淋巴酚氧化酶纯化，纯化倍数为41.5倍，酶得率为12.7%，比活力为4030.6U／mg，酶得到了初步纯化。

The enzyme was purified by 40 % saturated ( NH4 ) 2SO4 and Sephadex G-200 gel filtration from crude enzyme with purification factor of 41.5 , and yield factor of 12.7 % , and the specific activity 4030.6U/mg . The enzyme was purified partially .

通过下述的连续步骤将这两种形态的过氧化物酶纯化：硫酸铵分级沉淀，Sephacryls-200柱色谱和ConA-Sepha-Fose4B柱色谱。两种不同形式的过氧化物酶的纯化倍数分别为237和53倍。

The two forms of peroxidase have been purified 237 and 53 folds , respectively , through ammonium sulphate fractionation , and successive chromatography on Sephacryl S-200 and ConA-Sepharose 4B columns .

经过硫酸铵分级沉淀、SephadexG-100凝胶过滤层析和DEAE-sePharoseFF离子交换层析对大麦虫水溶性蛋白逐步分离纯化，并对所得的各组分的抗氧化性进行研究。

The water dissolved protein of barley pest was extracted by means of salt solution and was purified by ammonium sulfate precipitation with Sephadex G-100 gel filtration and DEAE-Sepharose FF chromatography . The antioxidant activity of each component was also investigated .

黄嘌呤氧化还原酶属于含钼脱氢酶黄素蛋白家族，是组成乳脂肪球膜的主要蛋白，通过硫酸胺分级沉淀和DEAE-Sepharose柱可从乳脂肪球膜中纯化。

Xanthine oxidoreductase ( XOR ), a complex molybdoflavoprotein , is major protein of the fat globule membrane in milk . XOR was isolated and purified from MFGM with ammonium sulfate fractionation and DEAE-Sepharose column chromatograph .

以番茄为试材，经2.4kJ/m2短波紫外线（UV-C）照射，取果皮经冰浴研磨、硫酸铵分级沉淀、凝胶过滤和离子交换层析，分离纯化苯丙氨酸解氨酶。

Phenylalanine ammonia-lyase ( PAL ) of tomato peel were induced after 2.4 kJ / m2 ultraviolet light-C ( UV-C ) irradiation . PAL was separated and purified By ice rubbing , sulfate grade deposition , gel filtration and ion exchange chromatography .

方法：应用硫酸铵分级沉淀、phenyl-sepharoseHR5/5层析等方法分离纯化长链脂肪酸醇氧化酶，应用SDS-PAGE电泳测定其相对分子量，并对其Km、酸碱稳定性及其底物特异性进行分析。

Methods : Fao was purified by ammonium sulfate fractionation and phenyl-Sepharose HR5 / 5 chromatography . SDS-PAGE was used to determine the molecular weight of fao . The Km , acidic and alkaline stability , and substrate specificity of the recombinant long-chain fatty acid alcohol oxidase were analyzed .

芽孢杆菌2（Bs-2）的发酵液经过硫酸铵分级沉淀、SephadexG-75凝胶过滤层析、Q-Sepharose阴离子交换层析、电泳制备和电洗脱，获得一种具有纤溶活性的蛋白酶；

A fibrinolytic enzyme was purified from the crude extract of Bs-2 , a strain of Bacillus sp . , through ammonium sulphate fractional precipitation , Sephadex G-75 gel filtration , Q-Sepharose anion exchange chromatography , preparative PAGE and electric elution .

方法采用酸、碱抽提、硫酸铵分级沉淀、膜过滤、色谱分离等技术，从1kg猪胰脏中同时分离纯化9种酶。

METHODS By using techniques such as acid and alkaline extraction , ammonium sulfate fractional precipitation , membrane filtration and chromatographic separation , up to 9 kinds of enzymes were simultaneously extracted and purified from 1 kg of porcine pancreas in one process .

硫酸铵分级沉淀结合多种层析技术，从螺旋藻（SP-Dz）中纯化到电泳纯别藻蓝蛋白（Allophycocyanin，APC），纯度（A650/A280）达4·83。APC在30min内的荧光扫描曲线为直线；

APC of electrophoresis-purity was obtained from Spirulina using ammonium sulfate grade precipitation followed with several gel chromatography , and the purity ( A_ 650 / A_ 280 ) of which attained to 4.83 . The fluorescence scan curve of APC was a direct line in 30 min ;

胡敏酸的酒精分级沉淀法分级

Fractionation of humic acid by fractional precipitation technique

用饱和硫酸铵分级沉淀法发现在20%~50%的饱和度之间沉淀出来的蛋白有抗菌活性。

The pellet precipitated by ammonium sulfate between 20 % ~ 50 % saturation showed antibacterial activity .

用乙醇分级沉淀的方法将人参多糖和黄芪多糖分级、精制。

Ginseng polysaccharide and Astragalus polysaccharide were separated into different grades by using ethanol of different concentrations .