分子原位杂交

方法：应用离体大鼠心脏人血浆体外灌注的异种移植超急性排斥反应模型，同时应用分子原位杂交技术进行c-myc基因信使核糖核酸（mRNA）在超急性排斥反应中的表达及细胞定位。

Method : Through the model of hyperacute rejection of xenotransplantation with isolated rat heart perfused with human plasma , we detected c myc mRNA expression and cell localization with in situ hybridization in hyperacute rejection .

方法：应用核酸分子原位杂交（ISH）技术，对26例PCa和9例正常前列腺（NP）组织中PSCAmRNA进行检测和定位。

Methods : Using nucleic acid in situ hybridization ( ISH ) technique , the expression of PSCA mRNA in 26 cases of prostatic adenocarcinoma ( PCa ) and 9 cases of normal prostate ( NP ) specimens were observed .

方法：应用地高辛标记的cRNA探针核酸分子原位杂交技术显示新生大鼠脑内转化生长因子β（transforminggrowthfactor-β，TGF-β）的mRNA表达。

METHODS : mRNA expression of transforming growth factor-beta ( TGF - β) in the neonatal rat brain was shown by in situ hybridization which labeled the cRNA probes with the digoxin .

方法：以胰腺癌病人癌组织（50例）、胰腺癌转移灶组织（15例）为研究对象，采用免疫组织化学（SP法）和分子原位杂交法检测bcl2在胰腺癌中的表达。

Methods : The SP and molecular in site hybridization methods were adopted to detect the expression of bcl2 in 50 cases pancreatic cancer tissue and 15 cases pancreatic metastases .

方法采用核酸分子原位杂交，应用RNA探针对32例TCC组织与8例正常组织的DDX24基因表达情况进行检测。

Methods The expression of DDX24 new gene was detected by using in sity hybridization with RNA primer in32 cases of TCC and8 cases of normal bladder tissue .

方法：建立大鼠腹主动脉瘤灌注模型，应用免疫组织化学和分子原位杂交技术检测MMP-3的表达。

Methods : The expression of MMP 3 were observed in 8 rat AAA models and 8 normal control aortic tissues using immunohistochemistry and molecular in situ hybridization technique .

用核酸分子原位杂交的方法，检测了WHV／myc转基因小鼠肝癌发生过程中c－jun、c－fos和c－H－ras基因的表达情况。

In situ hybridization was used with specific molecular probes to detect the expression of c jun , c fos and c H ras genes during hepatocarcinogenesis in WHV / myc transgenic mice .

远缘杂交的分子基础&DNA原位杂交

Molecular basis of remote hybridization & dna hybridization in situ

近年来，随着分子遗传学及荧光原位杂交（FISH）技术的发展，人们对微核有了进一步的认识。

With the development of molecular genetics and fluorescence in situ hybridization ( FISH ), more have been known about micronucle .

耐盐番茄分子育种及染色体原位杂交分析

Molecular Analysis of Genetically Engineered Tomato with High Salt Tolerance