内酶

研究过程中对ZY菌株进行了粗酶液的提取，提取的胞内酶和胞外酶对己内酰胺都有较好的降解效果。

Crude enzyme was extracted from stain of ZY . Either the endoenzyme or the exoenzyme has an excellent effect on degradation of caprolactam .

酶的定域实验表明，该菌中噻吩磺隆水解酶为胞内酶。

The distribution experiment showed that the thifensulfuron-methyl hydrolysis enzyme in the bacterium was endoenzyme .

首次发现高浓度NAC具有固定作用：细胞形态完好，而膜完整性及通透性被破坏、失去对Glu细胞毒性反应、细胞内酶活性丧失。

It is the first report that NAC at higher concentration can fix tissue .

“作为一个科学家，我们为这一发现兴奋，这是因为它是调节细胞内酶的作用基本的方法”Dr。

" As scientists , we 're excited about this discovery because it 's a fundamentally new approach to regulating what enzymes do in cells ," states Dr.

并且通过实验发现菌株S1的海藻糖合成酶为胞内酶，酶活力为7.30u/g菌体。

The trehalose synthase of S1 was detected to be an intracellular enzyme with the activity of 7.30 u / g wet cells .

其主要问题是总酶活相对较低，且胞外CAT产量不高，经细胞破碎获取胞内酶成本较高，不适合工业化生产的应用。

However , the major problem was that the total enzyme activity was not very high and the extracellular CAT production was low , requiring great cost for cell rupture in order to obtain the intracellular enzyme .

结论：DAO是敏感的肠上皮细胞内酶，其活性变化能反映创伤后小肠粘膜屏障功能受损和修复情况。

Conclusions : These data suggest that intestinal barrier function is damaged during the early stage after trauma and plasma DAO activity may be a useful marker of intestinal injury after trauma .

用双向电泳分离两种细胞蛋白质混合物并进行比较，找出差异点，这些点经过胶内酶切后进行MALDITOF质谱鉴定。

After in_gel protein digestion , the different_expressed spots were detected by matrix_assisted laser desorption ionization_time of flight mass spectrometry ( MALDI TOF MS ) .

是一种胞内酶，完整细胞呈现较低的酶活，而经过乙醇、乙醚、甲苯、曲拉通-100和CTAB处理的细胞酶活比完整细胞和超声波破碎的细胞表观酶活力均增加。

The whole cells of Lactobacillus plantarum exhibited low enzyme activity , but cells treated by ethanol , ether , toluene , Triton X-100 and CTAB apparently have higher activity of enzyme .

结论：乙醇能刺激乙肝表面抗原、e抗原以及核心抗原的表达，同时细胞内抗病毒基因MxA表达亦增加，可能与乙醇非特异性增强细胞内酶的活性，加强细胞内合成代谢有关；

CONCLUSION : Ethanol can induce the overexpression of HBsAg , HBeAg and HBcAg , and enhance the expression of antiviral gene MxA which may increase the activity of enzymes of synthetic metabolism .

挖取其中几个差异相对明显的蛋白质点，首先进行胶内酶切，再对获得的肽段进行MALDI-TOF质谱分析；

Several obviously comparable protein spots were diged out , and digested in gel , then the extracted peptide segments were analyzed with MALDI-TOF mass spectrometry .

从土壤中筛选的菌株ZJF-1所产β-葡聚糖酶的最适反应温度为65℃，有较宽的温度范围内酶活性较高，经鉴定该菌株为枯草芽孢杆菌（B.subtilis）。

The p-glucanase producing strain was isolated from soil and the optimal temperature of p-glucanase was 65 ℃ and it was identified as Bacillus subtilis .

Cu-MMT可使细菌细胞膜受损，胞内酶诸如冬氨酸氨基转移酶、乳酸脱氢酶和丙氨酸氨基转移酶等外泄。

Cu-MMT could destroy bacterial cellular membrane and then resulted in leakage of intracellular enzymes such as asparate aminotransferase , lactate dehydrogenase and alanine aminotransferase .

通过大鼠比目鱼肌体外实验，探讨牵拉负荷导致的肌肉损伤与肌细胞内酶的流出，骨骼肌细胞线粒体钙浓度（MCC）以及细胞脂质过氧化反应之间的关系。

In order to study the relationship of muscle injury , the release of intracellular enzyme , muscle mitochondrial [ Ca 2 + ] ( MCC ) and lipid peroxidation through the stretch of rat soleus in vitro .

反应器稳定运行后，其填料颗粒表面的扫描电镜照片表明，PBS外表面在降解菌体内酶的降解作用下，形成了多级孔状结构及大量空洞。

After stable operation of the reactor , the filler particle surface SEM photos show , PBS outer surface of the bacteria in the degradation of the enzyme degradation in vivo , the formation of a multi-level pore-like structure and a large hole .

对粗酶的基本性质进行了研究，酶的最适作用温度和最适PH分别是30℃和7.5。在pH5～8范围内酶的稳定性较好，35℃以下比较稳定。

The enzymatic characterization of crude enzyme showed that the enzyme is stable between pH 5 and 8 , and the optimum pH is 7.5 . The optimum temperature is 30 ℃ and the enzyme is stable when the temperature is below 35 ℃ .

牛精子蛋白胶内酶切条件优化及问题分析

Optimization and Analysis of In-gel Digestion with Bovine Spermatozoa Protein

高强度超声治疗对肿瘤组织内酶活性的影响

Effect of High Intensity Ultrasound Treatment on the Enzyme Activity in the Tumor

微生物胞内酶（细胞）在气升式生物反应器中的大规模生产

Large Scale Production of Intercellular Enzyme in Airlift Bioreactor

用非离子去污剂提高胶内酶切效率的初探

Improvement of In-gel Digestion by Adding Non-ion Detergent

AM真菌和胞囊线虫对大豆根内酶活性的影响

Influence of arbuscular mycorrhizal fungi and Heterodera glycines on enzyme activity in soybean roots

舒必利对抑郁大鼠模型脑内酶的影响

Sulpiride effect on the enzyme activeness in the brain of model rat with depressive disorder

胃癌患者胃液及胃粘膜内酶和免疫蛋白等的改变

Study of Enzymes and Immunoglobulins in Gastric Juice and Gastric Mucosa in Gastric Cancer Patients

毛白杨抗虫、感虫无性系树皮内酶活性的初步研究

Preliminary study on the enzyme activities in the barks of Populus tomentosa zoophobous and insect-attacked clones

褐斑病胁迫下内生真菌感染对高羊茅叶内酶活性的影响

Effect of Endophyte Infection under Brown Patch on POD and Chitinase Enzyme Activity of Tall Fescue

他说，快餐油炸食品引发的人体内酶的变化类似肝炎。

He said the changes in liver enzymes are in line with the effects of hepatitis .

结果：小肠粘膜细胞内酶活性远高于口腔粘膜细胞中的酶的活性。

The results showed that proteolytic enzymes in the buccal mucosa were less active than in the intestine .

酶的定域试验表明，该菌株的相关降解酶为胞内酶。

The enzyme distribution experiment showed that the enzymes relative SDBS degradation in the bacterium was intracellular one .

目的研究口腔粘膜内酶对胰岛素口腔吸收的影响。

AIM To evaluate the effect of proteolytic enzymes on the absorption of insulin in the buccal mucosa .

冷休克和冷冻对猪精子微细结构和精清内酶的影响

The effects of cold shock and freezing-thawing on the microstructure of spermatozoa and enzymatic activities in the seminal plasma of boar