免疫磁珠法
- 网络macs;minimacs;immunomagnetic beads;immunomagnetic bead separation
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免疫磁珠法是高效纯化T细胞的新方法。
MACS is a new purifying method of high efficiency .
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与其他分选方法相比,免疫磁珠法具有分离效率高,细胞活性好,操作简便等优点。
MACS technology has many advantages over other cell sorting methods , such as high efficiency , high cell viability and easiness to operate .
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免疫磁珠法在肾移植HLA快速分型中的应用
Application of Immunomagnetic Beads Technique for Tissue Typing in Renal Transplantation
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应用免疫磁珠法分离脐血CD(34)~+造血细胞
Immunomagnetic positive selection of cd_ ( 34 ) ~ + progenitor cells from human cord blood
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羟基淀粉沉淀对免疫磁珠法分离脐血CD(34)~+细胞及造血祖细胞增殖功能的影响
Colony culture and purification of CD_ ( 34 ) ~ + hematopoietic stem / progenitor cells from cord blood by MACS and HES
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免疫磁珠法分选小鼠肝脏Kupffer细胞及其特性观察
Isolation and purification of mouse liver Kupffer cells by immunomagnetic bead separation
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分离骨髓单个核细胞,用免疫磁珠法富集CD34+细胞、CD4+(Th)细胞。
Bone marrow mononuclear cells ( BMMNC ) were isolated and CD34 + and CD4 + cells were enriched by magnetic beads respectively .
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〔方法〕采用PCR法、免疫磁珠法和神奈川现象培养基三者结合使用。
〔 Methods 〕 PCR was used in combination with immunomagnetic separation and Kanagawa phenomenon culture medium to determine the isolation rate of TDH + V.p strains from environment .
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方法:采用美国NIH标准微量淋巴细胞毒技术和免疫磁珠法分离TB淋巴细胞。
Methods : T , B lymphocytes were isolated by American NIH standard lympho cytotoxic technique and magnetic beads method .
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用Percoll密度梯度及免疫磁珠法分离骨髓巨核细胞;
Percoll density gradient and immunomagnetic beads methods were used to purify megakaryocytes from bone marrow .
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方法应用免疫磁珠法(MACS)分选C57BL/6鼠CD4+CD25+T细胞。
Methods CD4 ~ + CD25 ~ + regulatory T cells were purified from C57BL / 6 mouse and using MACS .
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结论免疫磁珠法是分离脐血CD133阳性细胞的较好的方法。
Conclusions Magnetic cell sorting was a good method for separating CD133 + cell from cord blood .
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方法采用免疫磁珠法测量转染WeelHu基因的NIT细胞培养上清中的胰岛素水平及观察其增殖速度。
Methods The level of insulin in culture supernatant of NIT cells was measured by immune magnetic bead method and their growth rate was observed .
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结论:本实验通过免疫磁珠法(CD11b负选)成功地从FVB/N小鼠骨髓细胞中分离纯化出骨髓间充质干细胞。
CONCLUSION : MSCs are isolated and purified successfully from bone marrow cells of FVB / N mice with immunomagnetic beads method ( CD11b negative selection ) in this trial .
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方法:1、应用CD34+干细胞分选试剂盒和免疫磁珠法,经磁式细胞分选器(MACS)分离出人脐血CD34+干细胞;
Methods : ( 1 ) CD34 + hematopoietic stem cells were isolated from healthy human cord blood using CD34 + progenitor cell isolated kit by a high-gradient magnetic cells sorting system ( MACS ) .
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用Ficoll-Hapaque分离法分离动员的外周血(MPB)单个核细胞,免疫磁珠法分离纯化CD34+细胞,并将其在含胎牛血清的液体培养体系中、各组细胞因子诱导下培养15天。
Mononuclear cells were isolated from mobilized peripheral blood ( MPB ) by density gradient centrifugation over Ficoll . CD34 + cells were purified by using an immunomagnetic bead separation system .
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方法:选2例重型再障(severaplasticanemia,SAA)患者及1例正常人,用免疫磁珠法分离纯化外周血CD8+T细胞。
METHODS : CD8 + T cells were isolated from peripheral blood of two patients with severe aplastic anemia ( SAA ) and one normal person by Ficoll-Hypaque gradient centrifugation and then were purified by immunomagnetic microbead separation .
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方法利用免疫磁珠法(MACS)分离纯化脐血CD34+细胞,在无血清培养体系中以1×105/ml每孔接种,经不同细胞因子组合诱导培养。
Methods CD34 ~ + cells were isolated from umbilical cord blood by using a high-gradient magnetic activated cell sorting ( MACS ) system , and cultivated by 1 × 10 ~ 5 / ml in serum-free stem cell growth medium ( SCGM ) with different combination of HGFs .
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免疫磁珠法富集循环肿瘤细胞研究进展
New advancements in the enrichment of circulating tumor cells by immunomagnetic beads
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免疫磁珠法分离白血病转基因小鼠骨髓造血干细胞
Isolation of Bone Marrow Hematopoietic Stem Cells from PML-RAR α Transgenic Mice
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免疫磁珠法阳性检出率高于常规培养法。
The immunomagnetic separation has a higher positive rate than general cultivation method .
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免疫磁珠法分选出CD34+细胞。
CD34 + cells were divided by immunomagnetic beads selected system ( MACS ) .
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目的:为了建立免疫磁珠法体外分离提纯骨髓造血干细胞的方法。
Objective : To isolate and purify murine hematopoietic stem cell using the immunomagnetic microbeads .
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联用密度梯度离心与免疫磁珠法分离纯化人表皮朗格汉斯细胞
Separation and purification of Langerhans cells from human epidermis by density gradient centrifugation and magnetic cell sorting
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方法免疫磁珠法分选脐血中CD34+细胞,接种到预先照射的基质层上。
Methods Immunomagnetic selected CD34 + cells were inoculated onto the pre established irradiated human stroma layer .
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免疫磁珠法分选骨髓CD34~+细胞:纯度影响因素的分析
Immunomagnetic beads method for separating bone marrow CD34 ~ + cells : An analysis of purity influencing factors
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免疫磁珠法分离纯化骨髓间充质干细胞及向成软骨细胞分化
Isolation , purification and directed differentiation to cartilage cell of bone marrow mesenchymal stem cells by immunomagnetic beads method
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免疫磁珠法分选人骨髓多能成体祖细胞及初步鉴定
In vitro purification and culture of multipotent adult progenitor cells from human bone marrow by magnetic activated cell sorting
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为寻找更有效的体外扩增脐血CD34+细胞的造血细胞因子组合,采集健康产妇脐带血,用免疫磁珠法分选CD34+细胞。
The objective was to explore the optimal cytokine combinations for in vitro amplification of the cord blood CD34 + cell .
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免疫磁珠法检测系统性红斑狼疮患者外周血T淋巴细胞亚群及其意义
Detection and significance of CD4 and CD8 subsets of T lymphocytes in the peripheral blood of systemic lupus erythematosus patients by magnetic activated cell sorting