鲎

鲎C因子的性质、结构、功能及应用

The Property , Structure , Function and Application of Limulus Factor C

鲎C因子的研究进展

Research Advance in the Limulus Factor C

提示新型显色基质法鲎试验有助于对肺部G－细菌感染作出快速、灵敏的诊断。

Sensitive and useful method for the diagnosis of pulmonary G - bacterial infection .

鲎试剂法定量检测PVC一次性输液（血）器材中内毒素浓度

Quantitative Determination of Endotoxin Concentration in PVC Disposable Transfusion Set by Limulus Test

鲎血细胞多肽诱导HL-60细胞凋亡时线粒体膜电位的变化

Change of mitochondria membrane potential of HL-60 cells induced by horseshoe crab hemocyte peptides

用定量鲎基质显色法测定LaCl3结合内毒素（LPS）的能力。

The ability of LaCl 3 to bind lipopolysaccharide ( LPS ) was determined based on quantitative limulus test .

结果：纯化的LPS用紫外/可见光分光光度计扫描，呈单一吸收峰，并能被鲎试剂特异检出。

Results : Single peak of LPS is obtained using Violet spectrophotometer and LPS can be detected by limulus reagents .

我们的研究表明鲎G因子法可以用来快速有效地测定蘑菇多糖提取物中的（1,3）&葡聚糖含量。

Our study demonstrates that limulus Factor G test is a quick and convenient method for detecting ( 1 , 3 ) & glucan content in crude mushroom polysaccharide extracts .

鲎凝集素SDS电泳时表现出分子量为69000，和72000的二个亚基。

On SDS-PAGE , the lectin appeared to be composed of two heterogeneous subunits with molecular weight of 69000 and 72000 daltons respectively .

采用鲎试剂法测血浆LPS浓度；鲎试剂在抗淋巴细胞免疫球蛋白热原检测中的应用

The LPS concentration of plasma was detected by limulus lysate test . Application of Limulus Amoebocyte Lysate Reagent to Estimating Pyrogen in Antilymphocyte Immunoglobulin

探索静脉注射用人免疫球蛋白（IVIG）细菌内毒素含量的鲎试验检测方法。

To explore the limulus test in detection of the content of bacterial endotoxin in IVIG .

将菌株按属归类，伴放线嗜血杆菌的两株LPS鲎活性当量最高，平均为5.0190；

Classifying their species according to genera , the average limulus lysate activity equivalent of H.act LPS ( 5.0190 ) appears to be the hightest ;

中国鲎鲎素对人胃腺癌BGC-823细胞形态和超微结构的影响

Effects of Tachyplesin on the Morphology and Ultrastructure of the Human Gastric Carcinoma Cell Line BGC-823

鲎抗脂多糖因子的基因克隆及其在COS-7细胞中的表达

Cloning and expression in COS-7 cells of limulus Anti-Lipopolysaccharide factor Gene from Chinese Horseshoe Crab

采用这种方法，可以观察到人血清和血浆，鲎血浆，鲎阿米巴细胞溶解物（LAL）以及多粘菌素B对内毒素的定量灭活作用。

By using this method , the dose-dependent inactivation of LPS by human serum and plasma , Limulus plasma , LAL and polymyxin B was demonstrated .

方法：采用鲎试剂基质显色法定量测定内毒素，用放射免疫法测定ET，常规生化法测定血肌酐，同时留24小时尿液测定尿肌酐与尿钠。

METHODS : The plasma endotoxin , endothelin , creatinine , and 24 hours urine creatinine and sodium were measured by Limulus test method , radioimmunological method and biochemical analysis , respectively .

以内毒素标准品为参考标准，采用当显色基质重氮化法测定牙周病优势菌LPS的鲎活性当量。

The limulus lysate activity of LPS preparations were measured by the limulus chromo-genic diazotic assay and was calculated according to their correspondent reference test with standard endotoxin .

方法用鲎珠溶解物凝胶反应法对66名SOM患者共88份中耳积液进行内毒素含量的动态检测。

Methods The endotoxin content of 88 middle ear effusion from 66 patients with SOM was investigated with the Limulus assay technique at different stages .

mL-1的溶液后，可用灵敏度为0.25EU.mL-1的鲎试剂进行检测。

The limulus amebocyte lysate reagent with the sensitivity of 0.25 EU · mL-1 could be used in the test .

方法分别以基质显色法鲎试验和酶联免疫吸附测定（ELISA）检测肝硬化患者的血浆内毒素和LBP水平；

Methods : The plasma levels of endotoxin and LBP in cirrhotic patients were determined by chromogenic substrate Limulus amebocyte lysate assay and enzyme-linked immunosorbent assay ( ELISA ), respectively .

三刺鲎（Tachypleustridentatus）血淋巴的研究1.血蓝蛋白的分离及其免疫原性的研究

Studies on the horseshoe crab ( tachypleus tridentatus ) hemolymph 1.separation and immunogenicity of hemocyanin

结果：盐酸洛美沙星葡萄糖注射液对鲎试剂的凝集反应有干扰作用，调节药液pH值至68～72可消除干扰，用灵敏度为025EU/ml的鲎试剂可进行细菌内毒素检查。

RESULTS : Lomefloxacin and glucose injection could interfere with limulus test , however , the interference could be eliminated after adjusting the pH of injection to 6.8 ~ 7.0.The proper concentration of limulus test agent of bacterial endotoxin test was 0.25EU/ml .

结果：SAGM红细胞保存液经2倍稀释后对鲎试剂反应无干扰作用。

RESULTS : There was no interference action of twofold diluted SAGM fluid on limulus test .

采用鲎试剂凝胶法检测人参提取物和人参皂甙Rb1中的内毒素含量。结果表明人参提取物和人参皂甙Rb1对凝胶法检测内毒素无干扰作用，内毒素含最小于0.5EU／ml。

The results indicated that ginseng extract and ginsenoside Rbl did not interfere with amebocyte lysate assay and the concentration of endotoxin was less than 0.5EU/ml in the ginsenosides .

方法：将3批样品用两个不同厂家生产的鲎试剂，按《中国药典》2000年版二部附录E方法进行干扰试验考察。

Method : The interference test of 3 batches of Buflomedil Hydrochloride Sodium Chloride Injection with 2 kinds of limulus test agent was done according to the method in appendix of China Pharmacopoeia ( Volume ⅱ Appendix ⅺ E , 2000 edition ) .

采用鲎基质显色法测定BPI与抗体孵育30min后其结合内毒素能力的改变。

LAL test was used to test the ability of BPI binding to endotoxin after porcine BPI cultured with antibodies for 30 min.

结果表明用鲎试剂灵敏度（λ）0.5EU／ml相对应的有效浓度进行试验，有蛋氨酸等11种氨基酸无干扰作用。

P. ( Chinese Pharmacopeia ) 1995 , and other reference , Using concentration of sample solution vs to the sensitivity of LAL reagent is 0 . 5EU / ml , results shows that eleven individual amino acids have no interference .

方法：按《中国药典》（二部）2000年版规定，用不同厂家生产的鲎试剂（TAL）对SAGM红细胞保存液进行细菌内毒素检查法干扰试验。

METHODS : Interference test was carried on endotoxin detection of SAGM erythrocyte preserve fluid with test agent of limulus ( TAL ) .

方法30%TBSAⅢ度烫伤Wistar大鼠及对照组，取门、腔静脉血用鲎基质显色法定量LPS；

Methods Wistar rats inflicted 30 % TBSA H degree scalding were employed as the model . Serum LPS levels in portal vein and vena cava were determined by limulus amebocyte lysate ( LAL ) test .

基因工程蛋白类药物生产中常用的2-巯基乙醇（2－ME）、二硫疏糖醇（DTT）和还原型谷胱甘肽（GSH）对鲎试验显色反应有干扰作用。

It was found that the 2-ME , DTT and GSH that was usually used in the production of gene-engineering protein medicine have interference to the color reaction of quantitative LAL .