限制性核酸内切酶

限制性核酸内切酶与DNA相互作用研究进展

Progress on the Interaction of Restriction Endonuclease and DNA

Ⅱ型限制性核酸内切酶NcrⅠ的研究

Study on A Type ⅱ Restriction Endonuclease Ncr ⅰ

人乳头瘤病毒DNA的限制性核酸内切酶分析

Restriction enzyme analysis of human papilloma virus ( hpv ) dna from common warts

该法提取的血吸虫基因组DNA较纯，适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。

The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot .

本文应用限制性核酸内切酶HaeⅢ显示了孟氏裂头绦虫染色体G分带，获得了较好的G带带纹。

G-banding of the chromosomes meets with success by using restriction endo-nucleas Hae ⅲ in Spirometra mansoni .

HSV临床分离株的限制性核酸内切酶酶切分析与鉴定

Restriction endonuclease cleavage analysis of DNAs from herpes simplex verus clinical isolates

方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。

Methods : 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction ( PCR ) technique .

结果：PCR和限制性核酸内切酶图谱分析均证实所获重组质粒中含有P1蛋白基因。

Results : Recombinant plasmid was proved to carry P1 protein gene by using PCR and endonuclease map analysis method .

限制性核酸内切酶PstⅠ活性基团的研究

Studies on the Active Groups of Pst I Restriction Endonuclease

方法应用PCR技术扩增T细胞受体γ链基因重排，选用四种限制性核酸内切酶消化扩增产物。

Methods After amplifying T-cell receptor γ chain gene rearrangement using polymerase chain reaction ( PCR ), we digested the products with 4 kinds of restriction enzyme .

结果：（1）通过DNA测序和限制性核酸内切酶酶切鉴定证实，hMMP-12成功克隆到所需表达载体中；

Results : ( 1 ) The hMMP-12 was successfully cloned into the needed vector by enzymatic digestion and DNA sequencing ;

质粒pBR322的体外扩建以及有关限制性核酸内切酶图谱

In vitro Enlargement of pBR322 and the Related Restriction Enzyme Maps

人工核酸酶具有限制性核酸内切酶的高度专一性，能在人们预先设计的任何位点切割DNA，可应用于基因分离、DNA定位诱变、新的化学治疗等分子生物学领域。

Artificial nucleases have high selectivity just as endonucleases , they can cleave DNA at any locations that the chemists have designed , and they can be used in molecular biological areas such as isolation genes , DNA location inducers and new chemical therapy etc.

通过PCR扩增狂犬病病毒SAD-B19株的G、N基因。将回收的目的片段用限制性核酸内切酶PstI进行酶切。

The G and N gene of rabies virus SAD-B19 strain were amplified by PCR , the target genes were collected and digested by Confine digestion enzyme of PstI .

用X-gal平板及质粒图谱分析方法筛选重组克隆株，再用限制性核酸内切酶酶切图谱分析鉴定。

The recombinant clone was screened by X-gal plate and plasmid map , and identified by restricted enzyme mapping analysis method .

方法应用聚合酶链反应、DNA测序和限制性核酸内切酶酶切等技术对15个AR-JP家系先证者的parkin基因进行突变研究。

Methods the polymerase chain reaction ( PCR ), DNA sequence analysis , and restriction enzyme digestion analysis were applied to check parkin gene mutations of 15 index patients from 15 families with AR-JP .

其特点在于结合使用限制性核酸内切酶EcorI及纳米金标记的检测探针，能够很好的降低背景值并有效增强信号。

It features combined with restriction endonuclease ECoR I and nano-gold-labeled detection probe that can reduce the background of a very good value and effective way to boost the signal .

利用限制性核酸内切酶PstⅠ在不同的有机介质中的催化反应，研究了水含量、相对粘度，介电常数和渗透压等介质的物化性质对PstⅠ原活性和星号活性的影响。

Through the catalyzed reactions of the restriction endonuclease Pst ⅰ in different organic medium , the effect of the four physico-chemical properties such as total water content , relative viscosity , dielectric constant and osmotic pressure on the prime and the star activity of Pst ⅰ was studied .

单纯疱疹病毒基因组的限制性核酸内切酶分析

Analysis of Herpes Simplex Virus Genomes with Restriction Endonucleases

芽孢杆菌中的限制性核酸内切酶

The restriction endonucleases from Bacillus

我们从66株国内收集的芽孢杆菌中筛选出10株菌株，它们含有限制性核酸内切酶活力。

The activities of restriction endonucleases were found in10 out of66 strains of Bacillus isolated and examined in our country .