锚蛋白

目的：本研究探讨红细胞蛋白激酶C（PKC）活性变化对锚蛋白的影响。

Objective : To study the effect of protein kinase C ( PKC ) on ankyrin in erythrocytes .

采用间接免疫荧光标记方法观察PKC及锚蛋白的细胞内分布情况；

The cellular distribution of PKC and ankyrin was observed by indirect immunofluorescence .

血管内皮细胞蛋白激酶C与锚蛋白、CD44分子生物学特性之间关系的研究

Study on Relationship between PKC and Biological Features of Ankyrin and CD44 Molecules in Vascular Endothelial Cells

蛋白激酶C对血管内皮细胞锚蛋白、CD44表达及亚细胞分布的影响

Effects of Protein Kinase C on Expression and Subcellular Distribution of Ankyrin and CD44 in Vascular Endothelial Cells

利用间接免疫荧光标记和Western印迹方法分析蛋白激酶C活性对锚蛋白及CD44的亚细胞分布及蛋白质表达的影响。

Effects of PKC activity on subcellular distribution and protein expression of ankyrin and CD44 was analyzed respectively with indirect immunofluorescence and Western blot .

CD24属糖基磷脂酰肌醇锚蛋白。作为P-选择素配体的黏附分子，其可调节B细胞发育和神经发生。

Glycosylphosphatidylinositol-anchored membrane protein CD24 is a ligand of P-selectin and plays a role in B-cell development and neurogenesis .

最近克隆出来的JNK相互作用蛋白（JIP1）是一种能抑制JNK核移位的胞浆锚蛋白。

JNK interacting protein 1 ( JIP-1 ) was newly identified as a potent inhibitor of JNK .

目的探讨外周血细胞糖基磷脂酰肌醇锚蛋白（GPI－AP）缺陷与再生障碍性贫血（AA）和阵发性睡眠性血红蛋白尿（PNH）的关系。

Objective To investigate the deficiency of glycosylphosphatidylinositol-anchored pro-teins ( GPI-AP ) in patients with aplastic anemia ( AA ) and paroxysmal nocturnal hemoglobinuria ( PNH ) .

锚蛋白重复序列（ANK）是生物体中广泛利用的一种序列模体。

Ankyrin repeat ( ANK ) is a widely used sequence motif in organisms .

目的：探讨糖基磷脂酰肌醇锚蛋白（GPIAP）在骨髓增生异常综合征（MDS）患者外周血细胞上的表达情况。

Objective : To investigate the expression of glycosylphosphatidylinositol anchored proteins ( GPI AP : CD55 , CD59 ) on peripheral erythrocytes and granulocytes in patients with myelodysplastic syndrome ( MDS ) .

结论：检测血细胞膜表面GPI锚蛋白分子表达可为确定或排除PNH的诊断提供敏感、特异的证据。

However , lymphocytes with PNH defect were found in only 14 patients . Conclusion : GPI anchored protein assay provided sensitive and specific method for the diagnosis of PNH .

目的：探讨血细胞膜表面糖基磷脂酰肌醇（GPI）锚蛋白分子表达异常在诊断阵发性睡眠性血红蛋白尿症（PNH）时的意义。

Objective : To detect the expression of glycosyl phosphatidyl inositol ( GPI ) anchored protein on the blood cell membrane and its implication in the diagnosis of paroxysmal nocturnal hemoglobinuria ( PNH ) .

水稻锚蛋白基因家族分析和锚定膜蛋白的表达模式研究

Analysis on Rice ANK Gene Family and Expression Pattern of Ankyrin-transmembrane Genes

综述了近年来有关锚蛋白参与植物信号传导的研究进展。

In this review , we discussed the research progress of ankyrin repeat containing protein in plant signal transduction .

锚蛋白重复序列模体是生物体内最普遍的蛋白质序列模体之一，在多种细胞活动中主要介导蛋白质与蛋白质的相互作用。

Ankyrin repeats , as one of the most common protein motifs , are involved in diverse protein-protein interactions in various life activities .

脂微区为近年发现的广泛存在于质膜，主要由糖鞘脂、鞘磷脂、胆固醇及多GPI锚联蛋白组成的局限性结构域。

Membrane lipid microdomain is a newly discovered membrane confined domain which consists of sphingomyelin , cholesterol , glycosphingolipid and many GPI anchored proteins .

再以带有FLAG标签的南极假丝酵母脂肪酶B蛋白的C末端同3种锚定蛋白N端融合，并利用免疫荧光显微镜检测，证明新构建的毕赤酵母展示通用载体是成功的。

Then tagged with a FLAG Candida antarctica lipase B protein with three kinds of C-terminal N-anchored fusion protein and used immunofluorescence microscopy , that the newly constructed yeast display common carrier is successful .

神经元GPI锚定蛋白结构与功能研究进展

A Brief View on the Structure and Function Study of the Neuronal GP-anchored Proteins

GPI锚定蛋白抗体CD55、CD59及RET在贫血诊断中的意义探讨

Investigation of anchoring protein antibodies CD55 / CD59 and RET on the diagnostic significance of anemic patients

此外，他们以抗CD146抗体和AnnexinV（锚定蛋白）作为标记，通过流式细胞仪测定了血样本中循环凋亡细胞的水平。

In addition , they quantified circulating apoptotic cells by flow cytometry of blood samples labeled with anti-CD146 antibody and Annexin V.

已知GPI锚定蛋白由磷脂酰肌醇，糖链和蛋白部分组成。一般认为影响GPI锚定蛋白在脂筏中定位的主要因素是GPI锚的脂质部分。

So making clear the location mechanism of GPI-anchored proteins in lipid raft and influencing factor is very important .

作为脂筏的组成成分，GPI锚定蛋白的功能与脂筏关系密切。GPI锚定蛋白在脂筏内外分布的不同常影响其功能。

As the main component of lipid rafts , the functions of GPI-anchored proteins are related closely to lipid rafts .

少突胶质细胞髓鞘糖蛋白是糖基磷脂酰肌醇锚合蛋白，富含亮氨酸重复片段结构域，参与蛋白间的相互作用，发挥DNA修复、RNA剪接、细胞间黏附及信号转导等功能。

OMgp , a glycosyl-phosphatidyl inositol anchor protein , was rich in leucine repeated segments , participated in interaction among protein , had the function of DNA repair , RNA splicing , cell adhesion , signal transduction and so on .

GPI锚定蛋白可以通过其尾部脂质GPI结构自然地整合到细胞膜上。

This method involves purified glycosyl-phosphatidylinositol ( GPI ) - anchored proteins which can spontaneously incorporate their lipid tail into cell membranes .

不同脂酰链对GPI锚定蛋白在脂筏中定位的影响

The Effects of Different Fatty Acyl Chains on the Distribution of Glycosylphosphatidylinositol ( GPI ) - anchored Proteins in the Lipid Rafts

胰岛素受体底物-1（Insulinreceptorsubstrate-1，IRS-1）作为多位点的锚定蛋白，连接受体激酶和信号蛋白质，引起受胰岛素调节的许多细胞内的多种功能的活化。

Insulin receptor substrate-1 ( IRS-1 ) acts as a multisite docking protein that binds signal proteins and links the receptor kinase to a number of cellular functions that are regulated by insulin .

在细胞表面，GPI锚定蛋白常与鞘脂（包括鞘糖脂和鞘磷脂），胆固醇等聚集在一起形成具有特定结构和功能的质膜微区，称GPI微域（GPImicrodomain）。

GPI-anchored proteins often gather with sphingolipids ( including sphingomyelin and glycosphingolipid ) and cholesterol to form special plasma membrane microdomains ( GPI microdomain ) .

细胞表面工程，又称GPI锚定蛋白转移法，是一种不依赖基因转移而能够在细胞膜上表达新蛋白的有效方法。

Cell-surface engineering technique , also named as GPI-anchored protein transfer , has become an attractive strategy to express new proteins on cell membranes without gene transfer .

结论：降低GPIPLD活性能增加骨髓单个核细胞对纤连蛋白的黏附率，与此同时，骨髓单个核细胞上GPI锚定蛋白CD24的表达增强。

It is concluded that depression of GPI-PLD activity can increase the adhesion rate of bone marrow mononuclear cells from the patients while the CD24 expression is enhanced .

结论溶血试验的发生机制可能与CD59等锚连蛋白的缺陷有关。蛇毒因子溶血试验在检出较小的CD59抗原缺陷方面最为敏感。

Conclusions Quantitative differences of CD 59 underlies the variability in hemolysis tests , and the CoF initiated hemolysis test is the most sensitive in detection of small CD 59 defects .