载体两性电解质
zài tǐ liǎnɡ xìnɡ diàn jiě zhì
- 名carrier ampholyte
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本实验的目的是采用载体两性电解质pH梯度等电聚焦电泳的方法,结合SDS-PAGE测定牛凝血酶的等电点。
The purpose of the experiment is to assay the isoelectric point of bovine thrombin by carrier ampholytes pH gradient isoelectrofocusing ( IEF ) electrophoresis and SDS-PAGE .
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对第一向为载体两性电解质pH梯度等电聚焦双向电泳(ISO-DALT)中若干影响分离效果的实验条件进行了研究.结果发现,琼脂糖和尿素的纯度明显影响分离效果;
The effect of several experimental conditions on the result of ISO-DALT was investigated in this paper .