转基因动物技术

转基因动物技术的基本方法和应用

Application of Transgenic Animal Technique and Its Basic Methods

本文主要概述了转基因动物技术的应用进展。

Advances in application of transgenic animal technique have been outlined mainly in this paper .

转基因动物技术与YAC（酵母人工染色体）克隆及定位突变技术的结合可望阐述大片段基因（簇）中不同部位对发育阶段特异性表达的调控规律。

Moreover , yeast artificial chromosome ( YAC ) cloning and site-specific mutation techniques can be combined with transgenic technique to investigate the developmental gene regulation of large genes ( clusters ) with mutations in different sites .

转基因动物技术在抗病毒育种中的研究进展

Studies on Transgenic Techniques Application in Anti-virus Disease Breeding

随着这些技术的不断发展，转基因动物技术应用正以其突出的优越性指导着多个领域的工作。

With constant development of the technology , the application of transgenic animals is guiding many fields with its outstanding superiority .

转基因动物技术作为一种研究基因功能的技术体系，在生命科学研究领域有着广泛的应用前景。

As a technical system of researching gene function , transgenic technology has a widely usage in the study of life science .

本文通过转基因动物技术的研究状况、目的、方法、用途及存在问题对该技术进行综述。

In this paper , the history , aim , methods , existed questions and application are discussed , to study the technology of transgenic animals .

转基因动物技术在分子免疫、免疫耐受、自身免疫、移植免疫、抗感染免疫和免疫防治等研究中的应用日益广泛。

There are more and more applications in integrating transgenic technology into immunology , especially in molecular immunology , immunological tolerance , autoimmunity , transplantation immunity , anti-infectious immunity , immunoprophylaxis and immunotherapy .

利用转基因动物技术可以建立人类疾病相关基因的转基因动物模型，以探讨异常基因的表达调控规律；

Transgenic technique can be applied to set up transgenic models for human genetic diseases from which expression regulation of abnormal genes are demonstrated and many pre-clinical data for human gene therapy will be provided .

转基因动物技术在建立动物模型，研究基因功能，治疗人类疾病，动物抗病育种，异种器官移植和生物制药等许多方面都具有广泛的应用价值。

Transgenic animal technology have a wide range of applications in the establishment of animal models to study gene function and treatment of many human diseases , animal breeding for disease resistance , xenotransplantation , biological pharmacy .

当人们将外源基因转染入哺乳动物细胞以后，总是希望外源基因能高效表达，并通过转基因动物克隆技术制作转基因动物，使其要求导入的外源基因在动物个体高水平表达所需要的新性状。

We always hope that the foreign genes could be highly expressed in mammalian cells and individuals when foreign genes are transfected into mammalian cells and the transgenic animals are made .

体细胞核移植技术为生产转基因动物核心技术之一，该技术的优化和成熟，对于成功生产转基因动物至关重要。

Somatic cell nuclear transfer ( SCNT ) is a key technique for producing transgenic mammalian , so optimization of this technique is important for improving the total efficiency of transgenic animal production .

近年来，随着转基因动物制备技术的日趋成熟与完善，建立转基因动物模型已经成为研究真核基因表达调控的一种重要手段。

Recently , with the development of transgenic technology , producing transgenic animal model has become an important way to study the expression regulation of eukaryotic genes .

制作转基因动物的显微注射技术

Technique of Microinjection for Making Transgenic Animals

转基因动物是现代生物技术中一个极其重要的研究领域，目前已经有转基因小鼠、兔、绵羊、山羊、猪、牛、鸡和鱼等多种转基因动物问世。

Production of transgenic animals is one of the most important fields in modern biotechnology . Up to now , there have been many transgenic animals , such as transgenic mice , rabbits , sheep , goats , swine cattles , chickens and fish and so on .

利用动物转基因技术和动物克隆技术，再结合基因打靶技术，人们开展了基因的定点修复、遗传缺陷的治疗、动物不利基因的敲除失活等一系列的动物基因组修饰的理论和应用研究。

With the combination of gene targeting , animal transgenic and animal cloning technologies , there have been a series of theoretical and applied research of animal genomes modified , such as fixed-point gene repair , the treatment of genetic defects and inactivation of animal unfavorable gene .

简要论述了转基因动物的概念、制作方法及应用领域，回顾了转基因动物技术的发展及现状，分析了转基因动物与克隆动物的区别。

In this paper , the authors briefly discussed transgenic animals ' concept , ways of production , its application field , and analyzed the differences between transgenic animals and clone animals .