血凝抑制试验
- hemagglutination inhibition test
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固相免疫吸附血凝抑制试验快速检测甲型肝炎病毒IgM
Rapid detection of hepatitis A virus antibody IgM by solid - phase immunosorbent hemagglutination inhibition test
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ELISA法检测小鼠免疫血清的抗体效价,以血凝抑制试验测定其免疫反应性。
The antibody titers in sera of the immunized mice were detected by ELISA and the immunoreactivity was measured by hemagglutination inhibition test .
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免疫后不同时段采血,用血凝抑制试验方法测定HI抗体滴度。
Different times after immunization blood hemagglutination inhibition test method for the determination of HI antibody titers .
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随后开展了麻疹免疫监测,应用酶联免疫吸附法(ELISA)检测麻疹和风疹IgM抗体,微量血凝抑制试验检测麻疹IgG抗体。
IgM antibody of measles was tested with ELISA and IgG antibody was examined with HI test .
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应用该试剂盒与血凝抑制试验(HI)对85份血清进行同步检测,两者符合率达86.2%。
85 serum samples tested with HI and ELISA .. The positive coincidence rate is up to 86.2 % .
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胶体金免疫色谱测试法与血凝抑制试验检测登革热IgM、IgG抗体的比较
Comparison of detecting for dengue fever serous IgM and IgG between colloidal gold immunoassay and HI test
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用血凝抑制试验初步筛选,PCR方法进行进一步的确认检测,对NA基因进行序列测定。
HI test of preliminary test , RT-PCR Methods for further validation testing . Then do the gene sequencing of the NA gene .
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采用ELISA和血凝抑制试验(HI)检测腹水mAb的效价;采用ELISA、HI、免疫荧光染色(IF)及westernblot鉴定mAb的特异性。
The specificity of the mAb was characterized by ELISA , HI test , indirect immunofluorescence ( IF ) staining and Western blot .
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凝血特异性试验、吸收放散试验、血凝抑制试验证明其仅对人H血型物质特异。
Based on hemagglutination specificity test , absorption and eluate test , and hemagglutination inhibition assay , it was showed to be specific only for the human H blood group substance .
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临床样品检测结果显示,该方法的敏感性要高于血凝抑制试验,从而建立了RHDV特异、敏感的RT-PCR检测技术,可应用于RHDV临床诊断及流行病学调查。
This RT-PCR can be used in RHDV clinical diagnosis and epidemiology study .
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鼠疫FI单克隆抗体致敏红细胞用于反向血凝抑制试验检测鼠疫FI抗体的试验研究
Detecting fi antibody of Y.pestis by counter hemagglutination inhibition test with fi McAb sensitized red cell
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应用常规双向免疫扩散试验,交叉血凝抑制试验和酶联免疫吸附试验(ELISA)对四个不同地区分离株间的血清学关系进行了比较研究。
Serological relationships of four different strains of RHDV were studied by using immuno double diffusion , cross hemagglutination inhibition and enzyme-linked immunosorbent assay ( ELISA ) .
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每周采血应用血凝抑制试验(HI)测定血清中PPV抗体效价。
Serums were taken once a week and tested by hemagglutination inhibition ( HI ) to measure the PPV antibody titer .
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方法用2000年以来流行的与当前流行的A1、A3、B三株流感病毒株的标准抗原与免疫血清作交叉血凝抑制试验,根据所得数据计算抗原比,进行抗原性变异幅度分析。
Methods Perform cross hemagglutination inhibition test on the standard antigens of influenza virus strains A1 , A3 and B epidemic since 2000 with the corresponding immunosera .
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对40份盲样采用琼脂扩散试验(AGP)、血凝抑制试验(HI)和反转录聚合酶链反应(RT-PCR)进行了检测。
40 blind samples were examined by agar gel precipitation ( AGP ), hemagglutination inhibitory ( HI ) test and reverse transcription-polymerase chain reaction ( RT-PCR ) .
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用MDCK细胞进行病毒分离,用血凝抑制试验对分离到的病毒标本进行分型鉴定。
Viral isolation was preformed in MDCK cells and hemagglutination inhibition assay was used for subtype identification .
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方法珠海地区养殖场人员血清277份,正常出入境人员血清270份,通过血凝抑制试验HI实验进行抗体检测。
Methods For detecting antibody to these two subtypes with HI test , 277 serum specimens were collected from poultry breeding workers , and 270 were from normal Entry-Exit passengers in Zhuhai port .
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方法用交互血凝抑制试验对毒株进行抗原性分析,提取病毒RNA,用一步法逆转录聚合酶链反应(RTPCR)扩增HA1(血凝素重链区)基因片段,产物纯化后测序并分析结果。
Methods The cross hemagglutination inhibition test was used for antigenic analysis , RNAs were extracted by one step RT PCR to amplify HA1 gene and then the PCR products were sequenced .
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用TEHA和粗HA作血凝抑制试验结果无显著差别。
When TEHA and crude HA were compared as antigen , hemagglutination-inhibition test of patients sera did not show any difference in titers .
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血清检测采用琼脂扩散(AGP)和血凝抑制试验(HI),病毒检测用鸡胚法鉴定。
The method of agar gel precipitation ( AGP ) and hemagglutination inhibitory ( HI ) was adopted in serum detection . The chicken embryo culture was adopted in virus detection .
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血凝抑制试验能特异性地抑制该单克隆抗体与人LDH-C4的结合;
Human LDH-C4 specifically inhibited the agglutination of the McAb with human LDH-C4 ( immuno-hemagglutination test );
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反向血凝抑制试验(RIHI)检测鼠疫F1抗体在疫情监测和疫源地调查中的应用
Reverse indirect hemagglutination inhibition assay ( RIHI ) for detecting F_1 antibody of Y. pestis in epidemiological surveillance and focus survey
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[方法]用流感病毒血凝抑制试验检测流感病毒血凝素的抗原性,对血凝素重链区(HA1)进行基因序列分析。
[ Methods ] Haemagglutin antigen of the influenza virus strain was detected by Haemagglutination-inhibition ( HI ) tests and genetic sequence of HA1 was analyzed .
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应用微量血凝抑制试验,Gm、Km谱血清和10种特异性抗D血清致敏红细胞,对上海地区2万名随机汉族献血者作IG同种异型抗体检测。
A total of 20.000 random donors of Chinese Han nationality living in Shanghai area were investigated for anti-bodies against IgG allotype by microhemagglutination inhibition test with panel sera of Gm , Km known type and 10 different special anti-D sensitized RBCs .
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二免后20d后再次采血,采用间接血凝抑制试验反应测定抗体情况,比较各组处理的免疫效果。
The separated serum was treated with indirect hemagglutination inhibition test reaction for the determination of antibody potency . The immunization effect compared between each other .
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用自制的中药提取物与新城疫B1株混合后免疫55日龄鸡,以血凝抑制试验测定新城疫抗体效价。
Chinese drug extract made in our lab was blended with Newcastle disease ( ND ) B 1 strain , and vaccinated 55 days old chickens . The ND antibody titer was detected by haemagglutination inhibition test .
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同时建立SV感染小鼠模型,通过血凝抑制试验观察2,3-吲哚醌对小鼠的免疫功能促进作用以及通过观察药物对染毒小鼠死亡率和染毒鼠肺指数的影响,观察2,3-吲哚醌对染毒小鼠的保护作用。
Its effect on immunological function was observed by hemagglutination inhibition test using sendai virus infected mice model ; its protection effect to mice which was infected Sendai virus was observed by mice mortality rate and lung index .
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本文用血凝抑制试验(HIT)检查野鼠型EHF患者病后1个月至22年的血清标本31份,血凝抑制(HI)抗体阳性25份,阳性率为80.65%。
31 serum samples from the patients with wild rat type EHF after attack of the disease from one month to 22 years were tested by hemagglutination inhibition test ( HIT ) . The antibody positive rate was 80.65 % ( 25 / 31 ) .
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与血凝抑制试验(HI)对比检测了54份猪血清临床样本,两法的阳性符合率为71.4%、阴性符合率为65.5%、总符合率为81.4%。
A comparison was made in parallel with HI test established under the same conditions for the 54 clinical specimens . These two methods had a general coincidence rate of 81.4 % , and the positive and negative rate are 71.4 % and 65.5 % respectively .
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用这两种抗原做ELISA及血凝抑制试验,测定了31份乙型脑炎病人双份血清中乙脑特异性IgG。
So , it was assumed that the adhering ability of viral antigen in ELISA is correlated with haemagglutinin titre . 2 single sera specimens and 31 paired sera from Japanese B encephalitis patients were tested both by ELISA with suckling mouse brain antigen and haemagglutination inhibition test .