菌落

并对重组质粒进行双酶切、菌落PCR及测序分析进行鉴定。

To verified the by restriction enzyme digestion , bacterial colony PCR , and sequencing analysis .

不同浓度营养物质对细菌菌落分形生长的影响

Effects of the Concentration Field of Nutrient on Bacterial Colony Formation

为进一步确诊，医院实验室必须培养一个细菌菌落。

To confirm the diagnosis , the hospital laboratory must culture a colony of bacteria .

目前来看，卧室地毯最脏，细菌和酵母菌整体水平达到每平方厘米140CFU（每平方厘米的菌落形成单位），地毯中还有大量的霉菌。

Bedroom carpets were by far the dirtiest , with a combined bacteria and yeast level of 140 CFU per cm ² ( colony forming units per cm ² ) uncovered within them , as well as heavy mould .

应用生物素标记的DNA探针通过菌落杂交试验检测沙门氏菌

Detection of Salmonellae by Colony Hybridization Assay with Biotin-labelled DNA Probes

菌落PCR法鉴定的重组率偏高，存在假阳性现象。

A high frequency of false-positives appeared in colony PCR identification .

经菌落PCR和质粒酶切鉴定后，将目的基因进行序列测定。

Recombinants were confirmed by colony PCR and restriction enzyme digestion .

单菌落PCR法直接快速鉴定重组克隆

Rapid Characterization of Recombination Clone by PCR Screening of Individual Bacterial Colonies

这篇文章包含菌落转移或菌落杂交，Southern杂交和Northern杂交以及生物芯片。

It contains colony lifts or hybridization , Southern and Northern hybridizations and microarrays .

结果文库扩增后得到28个阳性克隆，进行菌落PCR分析，均得到200～1000bp插入片段。

Results The amplified library contained 28 positive clones .

结论：菌落PCR可用于筛选阳性重组克隆。

Conclusion : Bacteria colony-PCR can be used in screening positive recombinant colonies .

一种利用菌落直接PCR扩增DNA并用于测序的实验方法.通过对引物的设计和菌液浓度控制，使PCR反应后的内容物对测序干扰减到最小。

A method of using colony PCR products to sequence directly by controlling primer design .

菌落PCR在大规模基因组测序中的应用

Colony PCR Apply to The Rice Genome Sequencing

随机挑取筛选的菌落超感染，用夹心ELISA检测超感染上清。

The supernatants harvested were detected by indirect sandwich ELISA .

目的应用以菌落为模板的聚合酶链反应（PCR）技术筛选插有小鼠Doc1R基因组序列的重组阳性克隆。

Objective To screen the Doc 1R gene recombinant plasmid by use of colony PCR .

将文库菌落印迹至尼龙膜，分区培养提取质粒DNA，建立基因池，并分别转染NIH/3T3细胞。

The plasmids in cDNA library and in gene pools were extracted and NIH / 3T3 cells were transfected respectively .

结果：5个克隆的菌落PCR及酶切鉴定均检测到目的基因。

Results : The inserted genes were detected in all the 5 clones after PCR and enzyme digestion .

接种前和处死前做肺CT扫描，观察菌落计数与X线影像显示是否感染、感染轻重的关系。

Every rabbits was given CT scan before injecting and killing in order to study the relationship between the counts and X-ray images .

目的评价菌落PCR法鉴定噬菌体抗体库阳性重组率的可靠性。

Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries .

Uγ－1突变株的菌落形态为菊花形，白色。

The colony morphology of U γ - 1 mutant is chrysanthemum type and it is White .

结果表明，单菌落PCR法是一个有效简便的鉴定重组阳性克隆的方法。

It showed that the method individual bacterial colonies PCR was an efficient , easy one that characterized recombination clones .

分别采用臭氧及甲醛熏蒸协同紫外线照射对手术室、换药室空气消毒。结果显示：用两种方法行手术室空气消毒后，空气细菌培养菌落数有显著性差异（P＜0.05）；

Comparison of the effects of air disinfection with ozone ( method 1 ) and with formaldehyde plus ultraviolet ( method 2 ) was made in operative and dressing rooms .

菌落荧光性为Fo型。

The fluorescence of colony belonged to Fo type .

本报告根据鼻疽菌光滑型菌落的荧光结构变异，将原型称为N型，其荧光变异型分为FR、Fb、Ts及NF等型。

Based on the colonial variation in morphology and fluorescent structure the original colony type of Ps.

结果初次血培养阳性时血小板计数和APACHEⅡ与预后密切相关，而与菌落归属无相关性。

Results Platelet counts in first positive blood culture and APACHE ⅱ scores were closely correlated with prognosis .

方法应用扩增小鼠Doc1R基因组序列时使用的引物，以基因重组扩增后得到的菌落为模板，直接进行PCR扩增。

Method The recombinant colonies were transfered into the PCR reaction mixture . The PCR primers were used for constructing mouse Doc 1R genomic sequence .

菌株的初步鉴定（1）依据菌落形态和孢子性状，A2菌株经形态学初步鉴定为青霉菌。

Identification of strains ( 1 ) A2 was identified as Penicillium by morphologic taxonomy .

结果如下：1.通过对桦褐孔菌W和D菌株的筛选，发现D菌株生长快，菌丝致密，多糖产量高，同时对其菌落形态和菌丝形态进行了比较，确定菌株D为出发菌株。

The results were as follows : Inonotus obliquus D with high grow speed , dense hyphae and high yield of polysaccharide was screened .

TTC在嗜酸乳杆菌菌落计数中的应用

Application of TTC for colony enumeration of Lactobacillus acidophilus

西瓜根围取样分析表明，Ta能够在根围定殖，并能稳定地达到2.8×107cfu／g土壤的菌落种群。

Ta could colonize on the soil of watermelon plant with the stable population density of 2.8 × 10 7cfu / g · soil .