膜片钳放大器

为精确记录细胞电生理信号，膜片钳放大器采用了放大、频率补偿、电容补偿及电阻补偿等一系列的补偿技术，电容补偿技术是其中关键技术之一。

In order to accurately record cellular electrophysiological signals , the patch clamp amplifier adopts a series of compensation technology including high-gain amplification , frequency correction , resistance and capacitance compensation and so on , wherein the capacitance compensation is one of the key technologies .

一种新型膜片钳放大器系统的设计

A Novel Patch Clamp Amplifier System Design

在逻辑控制模块中，通过采用FPGA新技术，来设计控制电路，实现对探头、膜片钳放大器主板的控制和参数设置；

In logic control , FPGA technology is applied to set the parameters of head stage and mainboard of patch-clamp .

利用膜片钳放大器系统对细胞膜上离子通道跨膜电流变化极其灵敏的特点，研究并构建一种新型的膜片电极支撑BLMs核酸传感器，对SEB基因进行快速、灵敏和特异的检测。

The biosensor was fabricated by utilizing patch-clamp pipette technology , which characterized in sensitive changes of ionic current though physiological cellular membranes .

方法利用膜片钳放大器系统对细胞膜上离子通道跨膜电流变化极其灵敏的特点，构建一种新型BLMs。

Methods One kind of novel BLMs was fabricated by using patch-clamp pipette technology characterized by sensitive changes of ionic current through cellular membranes , and confirmed by Gramicidin method .

膜片钳放大器是膜电容监测的重要工具。

Patch-clamp amplifier is an important instrument to investigate membrane capacitance .

第二代膜片钳放大器中的关键电路设计

Key Circuits Design of the Second Generation of the Patch-Clamp Amplifier

PC-Ⅲ型膜片钳放大器自动电容补偿的实现

Realization of Automatic Capacitance Compensation in PC ⅲ Patch Clamp Amplifier

一种新的膜片钳放大器监测通路设计方法

A Novel Design of Current Monitor Pathway for Patch-clamp Amplifier

膜片钳放大器自动校准方法的研究膜片钳在生命化学中的应用

Application of Patch - Clamp Technique in Life Chemistry

全自动膜片钳放大器参数控制系统

Parameter Control System for Automatic Patch - clamp Amplifier

膜片钳放大器瞬态电流伪差的自动补偿

Automatic compensation of capacitive transient current in patch-clamp amplifiers

全自动膜片钳放大器关键电路设计

Key Circuit Design of the Computer-Controlled Patch-Clamp Amplifier

所设计的自动校准程序模块能快速准确地获得系统相关参数，为提高膜片钳放大器测量的精度奠定基础。

The system parameters can be obtained quickly and exactly by the automatic calibration routine .

膜片钳放大器正是处理微弱电生理信号的有力工具。

The patch-clamp amplifier is just an extremely powerful tool for processing the small electrophysiological signals .

主要内容如下：1.研究并实现了计算机控制膜片钳放大器的自动校准算法。

It consists of following aspects : 1 . The automatic calibration algorithm of computer-controlled patch-clamp amplifier was studied and implemented .

传统膜片钳放大器中，电容补偿参数配置需手动完成，过程复杂、繁琐。

In traditional patch-clamp amplifiers , the parameters for capacitance compensation are manually configured according to the observation of experimenters , which is very complicated and discommodious .

首先对膜片钳放大器工作原理及补偿技术进行了深入研究，这是实现膜片钳自动化的基础。

The first one clearly elucidates the operational principle of patch-clamp amplifier and the compensatory technology , which are the fundamental for fulfilling the automation of patch clamp .

为降低成本和解决现有膜片钳放大器系统中PC机的干扰问题，研究了一种基于单片机的膜片钳放大器小系统。

In order to reduce cost and resolve the interferential problem with PC in existing patch clamp amplifiers , a small patch clamp amplifier system design based on microcontroller is studied .

膜片钳放大器实现计算机控制必须对关键电路进行改进或重新设计，需要改进和重新设计的部分关键电路有逻辑控制，可编程滤波器、刺激通道、电极电位偏移补偿等。

Some key circuit , which includes logic control , programmable filter , stimulus channel offset voltage compensation and so on , must be designed anew or modified for Patch-Clamp Amplifier being controlled by computer .

介绍了膜片钳放大器中快电容产生的机理，根据新的快电容电气模型设计出相应的补偿硬件电路和软件算法，并通过计算机实现自动补偿。

This paper describes the principle of the fast-capacitance in the patch-clamp amplifier , designs the C-Fast transient cancellation circuitry and algorithms according to the new electrical model , and realizes the automatic adjustment of the circuitry through the computer .

自动化膜片钳放大器采用计算机控制，通过对电容瞬态电流的分析，自动搜索补偿参数，实现了电容补偿的自动化，简化了实验过程、提高了实验效率。

The computer-controlled automatic patch-clamp amplifier , through analyzing the capacitance transient current , automatically seeks the parameters required by the compensation , which realizes the automation of capacitance compensation , simplifies the experimental process , and improves the experiment efficiency .

最后详细介绍了进行全自动膜片钳放大器系统全自动测试的意义及测试流程，根据被测试环节对频率响应的特性，分类分析了全自动测试的原理、测试方法及测试内容。

In the end , the signification and test flow of full test of the Computer-Controlled Patch-Clamp amplifier are expatiated , according to the characteristic of being tested sections , the principle and means of full test are analyzed in detail .