脱氧尿苷
- deoxyuridine
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方法采用5-溴-2′-脱氧尿苷结合的小鼠局部淋巴结细胞免疫化学方法和豚鼠局部封闭涂皮试验(Buehlertest)分别检测2,4-二硝基氯代苯和凡士林的致敏性。
Methods The murine local lymph node assay based on 5-bromo-2 ′ - deoxyuridine ( BrdU ) incorporation and Buehler test were used to test 2,4-dinitrochlorobenzene ( DNCB ) and geoline allergenicity .
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标本制备:两组大鼠于相应时间点前1d以增殖性细胞的标记物5-溴脱氧尿苷50mg/kg腹腔注射给药3次,间隔4h1次。
The preparation of sample : The rats in the two groups were treated with 5-bromium deoxyuridine 50 mg / kg , the marker of proliferation cells , through abdominal cavity for administration 3 times and once every 5 hours one day before the corresponding time point .
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流加底物dU对酶反应转化率无显著影响,表明该酶促反应为非2′-脱氧尿苷底物抑制型;
In addition , there was not substrate dU inhibition in enzyme reaction because the obvious change of conversion rate was not observed in dU fed batch process .
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饲养九周后终止试验,小鼠植入渗透泵灌注溴脱氧尿苷(Brdu)72小时。
Nine weeks after the dietary intervention , the animals were implanted with osmotic pumps for 72 hours to infuse bromodeoxyuridine ( BrdU ) .
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用5μmol/L的5溴2脱氧尿苷(BrdU)对培养的hMSCs进行连续培养标记。抗氯霉素单克隆抗体杂交瘤细胞株的筛选及金标试纸检测方法的建立
HMSCs were labeled with BrdU . Filtration of hybridoma lines of monoclonal antibody and development of colloidal gold - labeled strip for rapid detection of chloramphenicol
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5-氟-2′-脱氧尿苷(FUdR)是一种氟代脱氧核苷类似物,被广泛用于治疗各种肿瘤。
Floxuridine ( FUdR ) is a fluorinated deoxynucleoside analog , which has been used extensively in the treatment of a variety of tumors .
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方法取孕龄15~18d胎鼠脑组织,分离获得神经干细胞,在体外培养、扩增,并用5溴2脱氧尿苷(BrdU)标记。
Methods Neural stem cells were isolated from the brain tissue of fetal rats ( P15-18 ) and cultured , expanded in vitro , marked by BrdU .
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选取孕龄15~18天的Wistar大鼠一只,取胎鼠脑组织,分离培养神经干细胞,在体外培养、扩增,并在移植前一天用5溴-2脱氧尿苷(BrdU)标记。
Neural Stem Cells are isolated from the brain tissue of fetal rats which are obtained from a pregnant Wistar rat ( P15-18 ) and cultured . expanded in vitro . Before tranplantation the Neural Stem Cells are labeled with BrdU .
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目的探讨5-[125I]-2'-脱氧尿苷(125IUdR)对大鼠C6胶质瘤细胞中嗜银核仁组织原区蛋白(AgNOR蛋白)和增殖细胞核抗原(PCNA)表达的影响。
Objective To investigate the effect of 5 - iodo-2 ′ - deoxyuridine ( 125IUdR ) on the expressions of argyrophilic nucleolar organizer regions ( AgNOR ) proteins and proliferating cell nuclear antigen ( PCNA ) of C6 glioma cells in Rats .
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方法5溴2脱氧尿苷(BrdU)标记新生细胞结合免疫组织化学方法对脑切片进行BrdU、TuJ1及GFAP单标或双标染色。
Methods Injection of the thymidine analog 5 bromo 2 deoxyuridine ( BrdU ) to determine the extent of cell proliferation combined with single or double immunohistochemistry staining with antibodies BrdU , TuJ1 ( class ⅲ β tubulin , neuron specific early differentiation marker ) and GFAP .
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5-碘脱氧尿苷对活体中姐妹染色单体互换的一些效应
Effects of 5-Iododeoxyuridine on Sister Chromatid Exchanges in vivo
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5溴脱氧尿苷和端粒酶逆转录酶标记肺干细胞在新生鼠高氧肺损伤中的作用
Role of pulmonary stem cells labeled with bromodeoxyuridine and telomerase reverse transcriptase in hyperoxic lung injury in neonatal rats
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方法从新生儿脐带血分离出单个核细胞,体外培养并予5溴脱氧嘧啶尿苷(Brdu)标记48h。
Methods The mononuclear cells abstracted from cord blood of infants were cultured in vitro and marked with5bromodeoxyuridine ( Brdu ) for48 h.
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了解术前脱氧氟尿苷口服化疗对胃癌组织血小板衍化内皮细胞生长因子(PD-ECGF)表达的影响及意义。
Objective To investigate the relationship of the expression of platelet-derived endothelial cell growth factor ( PD-ECGF ) and survivin to apoptosis in pancreatic cancer .
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脱氧氟尿苷冻干粉针剂的抗肿瘤作用研究
The study of the antitumor effect of doxifluridine lyophilized powder for injection
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醛氢叶酸/脱氧氟尿苷和顺铂联合治疗晚期大肠癌的疗效观察
The Effect of CF / 5-FUDR and DDP Combined Chemotherapy for Advanced Colorectal Cancer
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照后尿中5种核苷(脱氧胞苷、脱氧尿苷、7-甲基鸟苷、肌苷、假尿苷)和一种游离的碱基(胸腺嘧啶)大幅度增加,随后在一周内逐渐减少。
Ten nucleosides and bases were effectively separated . Five nucleosides ( deoxycytidine , deoxyuridine , 7-methylguanosine , inosine , pseudouridine ) and one free base ( thymine ) were shown to increase significantly soon after irradiation , and then gradually to decrease in the following days .
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各组分别于相应时间点取脑组织,然后应用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法测定大鼠大脑皮层、海马神经细胞凋亡数目。
Brain tissues were obtained at corresponding time points in each group , respectively , and then the cerebral cortex and apoptotic number of nerve cells in hippocampus were measured with terminal deoxynucleotidyl transferase ( TdT ) mediated Dutp nick end labeling ( TUNEL ) .