胚胎培养

与单个胚胎培养组相比较，三个胚胎培养组的优质囊胚形成率增高（P0.05）。

Compared with single embryo culture group , three embryo group cultivation increased quality blastocyst formation rate ( P0.05 ) .

采用体外全胚胎培养模型和显微镜检查方法，对接触不同剂量氯化甲基汞的离体SD大鼠胚胎进行了病理学观察。

Morphological observations of teratogenicity of methylmercury chloride ( MMC ) on 9 5 day isolated Sprague Dawley rat embryos were studied with whole embryo culture in vitro and optical microscopy .

植入后全胚胎培养培养基最适pH值为7.5～8.2。

The optimum medium pH for WEC is 7.5 to 8.2 .

从胚胎培养开始就添加葡萄糖可提高胚胎的囊胚发育率（20.1%vs10.3%，P＜0.05）。

Culturing embryos with glucose from the beginning of culture improved the blastocyst formation rate significantly ( 20.1 % vs 10.3 % , P < 0.05 ) .

本文应用大鼠全胚胎培养方法检测了二硫化碳（CS2）的发育毒性。孕鼠于妊娠7、8两天经呼吸道吸入浓度为100mg/m~3的CS2，每天染毒4小时。

The whole embryo culture method was used in testing the development toxicity of carbon disulphide .

胚胎培养液和体外操作环境对IVF-ET妊娠率的影响

Influence of embryos culture environment on the pregnancy rate

胚胎培养箱换水间隔时间对IVF妊娠结局的影响

The effect of interval time of water changed in the incubator on pregnancy outcomes in IVF-ET

结果ES培养体系显著优于传统的胚胎培养体系（P<0.05）。

Results : The ES cell culture system was better than the conventional culture system for the embryo development ( P < 0.05 ) .

核移胚以胚胎培养液NCSU23及卵丘单层共培养体系进行分别培养，核移胚卵裂率无明显差异（30.06%、31.5%，P＞0.05）。

Third , when NT embryos cultured in NCSU - 23 , the development rate ( 30 . 06 % ) is no significantly different from those in cumulus single layer culture system ( 31 . 5 % ) .

将牛体外受精胚胎培养于化学成份确定的SOF+PVA的培养系统内，观察了不同PVA浓度对胚胎体外发育的影响。

Bovine IVF embryos were cultured to blastocyst in SOF + PVA , and the effects of different PVA concentration on development of IVF embryos were observed .

石蜡油对胚胎培养效果有一定影响，Sigma油可满足胚胎培养的需要。

The in vitro development of buffalo embryos was influenced by the quality of mineral oil and the mineral oil from Sigma is suitable to embryo in vitro culture .

目的：探讨胚胎培养液和体外操作环境对体外受精-胚胎移植（IVF-ET）妊娠率的影响，以便建立更适宜的胚胎体外培养体系。

Objective : To investigated the Influence in vitro embryos culture environment on the pregnancy rate so that to establish the more suitable culture system of embryos .

在早期胚胎培养中，添加10%BFF可显著提高ICSI胚的囊胚发育率；添加10%BFF比添加10%FBS对提高早期胚胎培养质量更为有效。

10 % BFF could significantly increase the blastocyst rate and BFF was more efficient than FBS for the early development in vitro of goat ICSI embryos .

目的为研究全反式视黄酸（atRA）的发育毒性及其可能的作用机制，本研究采用植入后体外全胚胎培养方法研究atRA对小鼠胚胎生长发育的影响。

Objective To investigate the developmental toxicity of all-trans retinoic acid ( atRA ) and its possible mechanism , the post-implantation whole embryo culture ( WEC ) was used .

采用着床后体外全胚胎培养方法，观察同型半胱氨酸（homocysteine，HCY）对大鼠胚胎生长发育的影响。

In order to investigate the effect of homocysteine ( HCY ) on the development of rat embryos , the post implantation whole embryo culture ( WEC ) technique was used .

应用全胚胎培养方法研究CrCl3对大、小鼠胚胎的发育毒性研究

Use of whole embryo culture technique for evaluating the developmental toxicity of CrCl_3 in mouse and rat embryos

本课题以昆明小鼠为研究对象，通过测定胚胎培养液中的H2O2含量来探讨ROS对早期胚胎体外发育的影响，为提高家畜胚胎体外培养效率提供可靠的理论依据。

By the way of detecting HA concentration of mouse embryo medium , we discuses the effect of ROS on the development block of the mouse early embryos and offered the reliable theories for enhancing the developing efficiency of the early embryos of the mammalian .

结论IVF-ET周期中，第1天胚胎培养液中存在蛋白氧化应激现象，高水平的AOPP与胚胎质量，IVF结局负相关，在ICSI周期中，表现尤为明显。

Conclusion In IVF-ET circle , oxidative stress occurred in day 1 embryo culture media ; high level AOPP correlates negatively to the quality of embryo and outcome of IVF , especially in the ICSI circle .

【结论】卵裂期胚胎培养液中sHLA-G的表达量与胚胎发育和种植的有关。

[ Conclusion ] sHLA G expression is closely related to embryo development and implantation of cleavage stage embryo .

结果：正常组胚胎培养液中EGF、IGF-的含量显著高于异常组，且EGF含量与胚胎生长发育的形态学指标呈正相关，但与着床指标相关不显著；

Results : 1 . The levels of EGF and IGF-II of the normal group were significantly higher than those of the abnormal . The level of EGF was positively related to morphologic indices of embryo development , but was not related to the implantation indices .

本研究采用体外着床后全胚胎培养方法，观察了V2O5对小鼠胚胎生长发育的影响。

In this experiment , the effects of V 2O 5 on mouse embryo cultured in vitro were studied . Vanadium Pentoxide was added to 48h culture of 8.5 day whole mouse embryos .

成熟液和胚胎培养液中同时添加100μMEDTA-Na对猪早期胚胎体外发育具有促进作用；

Addition of 100 μ M EDTA-Na to embryo culture medium is the most beneficial to the early development of porcine parthenogenetic embryos in vitro ;

本文应用小鼠全胚胎培养技术，通过妊娠d8分别ip5,10,15和20mg·kg-1环磷酰胺（CP），研究了该药对器官原基形成期胚胎的致畸作用，并对其致畸机理作了初步探索。

Using the mouse whole embryo culture technique , the teratogenesis induced by cyclophosphamide ( CP ) ( 5 , 10 , 15 , 20 mg · kg - 1 , ip ) and its mechanism were investigated in mice on d 8 of gestation .

桑葚胚及囊胚期单胚胎培养微滴中可检测到LeY，其中以单纯序贯培养组胚胎分泌量最低，而体内发育组及共培养-序贯培养组胚胎LeY分泌量间无显著性差异。

LeY excreted by one morula or blastocyst could be observed , the LeY concentration in simple sequential culture group was lowest and the concentrations were no significant difference between the other two groups , namely , co-culture + sequential culture group and in-vivo group .

猪卵母细胞体外成熟及胚胎培养的相关研究

In Vitro Maturation of Oocytes and Culture of Embryos in Pig

小鼠胚胎培养方法的研究

The Research of Technique of Mouse Embryos Culture in Vitro

囊胚期与卵裂期胚胎培养及移植的对照研究

Control Study on CultUre and Transfer between Blastocyst Stage and Cleavage Stage

环磷酰胺对全胚胎培养小鼠胚胎的细胞学影响

Cellular effects of cyclophosphamide on mouse embryos from whole embryo culture system

奶牛卵母细胞体外受精和胚胎培养研究

Study on Cow Oocyte in vitro Fertilization and Embryo Culture

山羊卵母细胞的显微受精及胚胎培养

Goat Oocytes Fertilization by Intracytoplasmic Sperm Injection and In vitro Embryo Culture