终止子

该载体含有CaMV35S启动子、NOS终止子及具有卡那抗性的NPTⅡ基因。

There are CaMV 35S promoter , NOS terminator and Kan R NPT gene elements in this vector .

将启动子片段分别以不同方向与gus报告基因和nos终止子融合，构建了瞬时表达载体。

Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation .

终止子进程的解决方法就是使用kill命令进行显式终止。

The solution to the problem of non-terminating child processes is to explicitly terminate them using the kill command .

实验以35S启动子和NOS终止子为研究对象。

35S promoter and NOS terminater was studied as the object .

要解决这类问题，脚本需要能够捕获或trap终止子进程一节讨论的信号。

To solve these problems , your script needs to be able to catch or trap some of the signals discussed in Terminating a child process .

我们根据其编码基因的启动子和终止子中的一致序列，设计了一对PCR引物，用一次反应即同时获得了这3个基因的克隆。

According to the consensus of their promoter region and terminator region , we designed one couple of PCR primers that can amplify all of three CBF genes by one reaction .

用此方法共检测了35S启动子、NOS终止子和抗除草剂（草甘膦）和抗虫（Bt）4种转基因成分。

Using PCR-GeneScan the four transgenic gene such as 35S promoter , NOS terminator , EPSPS and cry were detected .

核酸免疫最常用的载体是质粒DNA，借助其自身的启动子、终止子等以及宿主的转录翻译机制合成所编码的蛋白质分子。

Plasmids are the common vectors for DNA vaccines . By using promoter and terminator on the plasmids and the host cells'transcriptional and translational machinery , the synthetic proteins has natural conformation comparing to the prokaryotic systems .

应用根癌农杆菌胭脂碱合成酶基因（nos）终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ（nptⅡ）基因的二重PCR对样品进行转基因成分检测，结果均为阴性。

The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase ( nos ) terminator and Escherichia coli strain K12 neomycin phosphotransferase II ( npt ⅱ) gene in these samples were all negative .

且基因起始端Kozak序列完全，基因终止子已经突变、读码框正确。

In the gene start area , there was an intact Kozak sequence . Terminator of gene was mutated and the reading frame was correctly identified .

以转基因烟草为材料，通过DNA提取、PCR扩增获得NOS终止子片段产物，以重叠延伸扩增法制备该片段的竞争性模板，并进行酶切验证。

The fragment of NOS terminator was obtained through the operating of DNA isolation and polymerase chain reaction ( PCR ) amplification from genetically modified tobacco . And the competitive template was made through the method of overlap extension amplification . The PCR products were confirmed with restriction enzyme digestion .

通过PCR获得的编码HD5的阅读框序列为303bp，其中，酶切位点12bp，保护性碱基6bp，终止子3bp，编码HD5的序列282bp（94AA）；

The open reading frame of DEFA 5 obtained by PCR was composed of 303 bp ( restrictive site 12 bp , protective base 6 bp , terminator 3 bp , and coding sequence of HD-5 282 bp ) .

T（32）终止子在棒状杆菌基因表达中的功能

Function of the T_ ( 32 ) Terminator in Gene Expression in Corynebacteria

下面的例子将在父进程接收到信号时终止子进程。

The following example kills the children when the parent is sent one of the signals .

内源性终止子是凭借自身的序列信号即可实现转录终止功能的调控元件。

Intrinsic terminators are sequence elements which can terminate transcription in the absence of any additional factors .

终止子技术与生物安全

Terminator technology and biosafety

在酵母分泌表达系统中，启动子、分泌信号肽、终止子都是介导外源基因高效分泌表达的重要元件。

In the yeast secretory expression system , cis-elements containing promoter , signal peptide , terminer are efficient for gene expression .

在转基因杜氏藻研究中，合适的启动子与终止子是决定外源基因能否表达和控制表达量的关键元件。

Proper promoter and terminator are the key elements to determine and control the expression level of foreign gene in transgenic Dunaliella .

为保护转基因作物知识产权发展起来的终止子技术引起第三世界国家的强烈反对。

" Terminator technology ", which was developed for intellectual property protection of transgenic crops , meets with refusal seriously in the third world .

虽然转基因作物产生不育种子可能在解决转基因逃逸带来的生态风险上有益处，但这个收获与终止子技术可能给全球食品保障带来危机相比，两者就不能等量齐观了。

Sterile seeds produced by genetically modified crops with terminator genes may solve the ecological risk brought about from transgene escape , but the accompanied risk on biosafety and expense of global food security is no solution .

两个终止密码子导致的遗传性蛋白C和蛋白S缺陷症

Gene analysis of hereditary protein C and protein S deficiency in two Chinese pedigrees

TGA不再是终止密码子X而编码色氨酸W。癌基因中的密码子使用

TGA encodes tryptophane and not the terminate code ; The Codon Usage in Oncogene Sequences

所分析的序列中裙带菜终止密码子的使用偏好TGA。

The stop codon usage preferred to TGA for U. pinnatifida .

在3D编码区末端有整个ORF的终止密码子TAA。

There is terminate codon TAA of the whole ORF at the 3D coding region termination .

有趣的是，Sec是由终止密码子UGA编码。

Interestingly , selenocysteine is encoded by codon UGA .

根据α-珠蛋白基因起始密码子上游及终止密码子下游的保守序列设计引物，以牦牛基因组DNA为模板克隆并测定了α-珠蛋白基因序列。

The α - globin gene of yak was amplified and cloned by using the primers based on the conserved region of the upstream from the initiation codon and the downstream from the termination codon .

E.coli和Yeast基因起始与终止密码子邻近序列碱基保守性、关联性的对比研究

The Contrast Study of Base ′ s Conservative Character and Correlative Character between E.coli and Yeast Gene for Adjacent Sequence of Initiation Codon or Termination Codon

开放读码框末端有由TAA组成的终止密码子。

The termination codon was composed of TAA .

起始密码子为ATG，终止密码子为TAA。

The start codon of the COX ⅰ is ATG , while the termination codon TAA .

分析结果在表明原核与真核分析对象的平均码距在起始密码子附近和终止密码子附近均呈现出显著变化，在原核生物的SD区域也有显著变化。

We observe that average code distances fluctuate obviously near the initiation codon and termination codon . Remarkable changes also appear in the SD field of procaryotic organisms . 2 .