线性基因组
线性基因组
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重组质粒与昆虫杆状病毒线性基因组混合,转染到昆虫细胞(Sf-21)进行基因重组,经3次病毒蚀斑克隆,获得高效表达Cap蛋白的重组杆状病毒,毒价可达1.28×108pfu/mL。
The insect cells ( Sf-21 ) were transfected with a mixture of linearized baculovirus DNA and the recombinant plasmid . A titre of 1.28 × 10 ~ 8 pfu / ml recombinant baculovirus expressing the Cap protein was obtained latter after three circles of plaque cloning .
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人类腺病毒有将近50种不同的血清分型,它们虽然有着相似的蛋白质样外壳形态和线性双链DNA基因组,然而它们之间却有着很重要功能的差别。
They share similar proteinaceous capsid morphology and linear double-stranded DNA genomes . However , important functional differences have been observed among the Ad serotypes .