紫草素

方法应用MTT比色法检测新疆紫草素对细胞的增殖、黏附抑制作用，用流式细胞术（FCM）、Annexin-V-FITC联合法及透射电镜检测新疆紫草素对胃癌细胞的凋亡诱导效应。

The effect of inducing apoptosis of the alkannin to the cells were observed by the flow cytometry ( FCM ), Annexin-V-FITC staining and transmission electron microscope .

结果表明，采用等温变压二级分离，在萃取压力为20MPa，萃取温度为35℃时，紫草素提取率为1.82%，其重金属及砷含量均符合WHO/FAO规定指标。

Using supercritical carbon dioxide was researched . The experiment showed that the extraction rate of alkannin was 1.82 % when the operation pressure was 20 MPa at 35 ℃, and the contents of heavy metal and As were met what WTO / FAO required .

紫草素诱导人白血病细胞K562的凋亡

Shikonin induces apoptosis of human leukemia K562 cells

紫草素诱导HeLa细胞凋亡经过caspase激活的机制

Shikonin induce HeLa cell apoptosis via a caspase-dependent mechanism

目的：研究紫草素对DNA拓扑异构酶I催化活性和K562白血病细胞凋亡的影响。

AIM : To study the effects of shikonin on the catalytic activity of DNA topoisomerase I and apoptosis of K562 leukemia cells .

结论新疆紫草素能够有效抑制胃癌细胞的增殖、黏附，并诱导凋亡。Iso的相序。

CONCLUSION The iso-alkannin and its derivatives could effectively inhibit the proliferation and adhesion of the gastric cancer cells and induced apoptosis .

紫草素对绒癌JAR/MTX细胞的耐药逆转及MRP、LRP表达的影响

The Effect of Shikonin on Reversing Drug-resistance of Choriocarcinoma JAR / MTX Cells and the Expression of MRP and LRP

本研究观察了紫草素（Shikonin）对小鼠肝癌（Hepatoma22，H22）和Lewis肺癌（Lewislungcarcinoma，LLC）的放射增敏作用。

Mice Hepatoma 22 ( H_ ( 22 )) and Lewis Lung Carcinoma ( LLC ) were used for demonstrating the radiosensitizing effect of shikonin in this study .

为探明病毒侵染对烟草细胞膜的损伤作用，采用圆二色波谱仪（CD）分析了被TMV侵染的烟草细胞膜蛋白结构的变化，以及右旋紫草素对感病烟草细胞膜的作用。

The changes of secondary conformations of tobacco cell membrane protein after TMV-infection was investigated by CD Spectra Technique and the changes in membrane molecular conformations was checked .

紫草素诱导人绒毛膜癌JEG-3细胞凋亡机制的研究

Mechanisms of Shikonin-induced Apoptosis in JEG-3 Cells

结果证实，在一定范围内，自制增溶剂WP能显著提高紫草素的溶解性能以及羊毛的表观染色深度和色泽鲜艳度。

It was found that self-made solubilizing agent WP could improve the solubility of shikonin and the apparent dyeing depth and the brightness on wool fabrics .

建立LC-ESP-MS方法，对紫草素大鼠肝微粒体体外温孵之提取物进行分离并对其结构作初步推断。优化了色谱柱、固定相、流动相组成及流速，以匹配LC-MS。

A LC-ESP-MS method was developed and used to directly separate the in vitro incubation extracts of shikonin and the structures of metabolites were proposed .

方法：利用体外细胞培养技术、mtt法、流式细胞仪、荧光显微镜技术检测不同浓度的紫草素对colo-16细胞株增殖及凋亡的影响。

Methods : the effects of shikonin on proliferation and apoptosis of colo-16cell line were assessed using culture technique of colo-16in vitro , mtt , fluorescence testing and flow cyctometry assay .

然而，紫草素对人正常角质形成细胞（HaCaT）的生长影响很小，表明紫草素具有抗肿瘤活性的同时，对人正常细胞有较低的毒性。

Moreover , shikonin had less influence on non-tumorigenic human keratinocyte ( HaCaT ) cell growth , suggesting that shikonin had anti-tumor activity and less side effects on human normal cells .

紫草素对晚期胶原性关节炎的作用研究

Regulation role of Shikonin in late stages of murine collagen-induced arthritis

光谱法与分子模拟对左旋紫草素和人血白蛋白键合作用的研究

Study on Shikonin-Human Serum Albumin Interaction by Spectroscopic and Molecular Simulation Methods

紫草素对大鼠佐剂性关节炎滑膜炎症的影响

Effects of shikonin on synovitis of adjuvant arthritis rats

渗透促进剂对紫草素透过大鼠腹部皮肤的作用

Effect of Penetration Enhancer on the Permeation of Shikonin in Rat Abdomen Skin

β-紫草素复方的药代动力学研究

Study on the compound pharmacokinetics of β - shikonin

紫草素对人胎肾系膜细胞凋亡的影响

Effect of Shikonin on Human Fetal Mesangial Cell Apoptosis

丙二醇增强涂膜剂中紫草素的经皮渗透效果；

Propanediol has a significant enhancing effect on the percutaneous absorption of shikonin .

紫草素对角质形成细胞株增殖的抑制及凋亡的作用

Regulatory effects of shikonin on inhibiting proliferation and inducing apoptosis of keratinocyte line

紫草素提取、转化、精制工艺研究

Study on the Technological Processes in Shikonin of Extracting 、 Convertion and Purification

左旋紫草素抗炎作用与其抑制蛋白酶体活性关系的研究

The Study of Association of Anti-inflammatory Effects of Shikonin with Its Proteasome Inhibition

新疆紫草素对肿瘤细胞生长抑制作用的研究

Study of suppression effect of Xinjiang shikonin inhibitor on the growth cancer cells

本论文对左旋紫草素（以下简称紫草素）的代谢转化进行了研究。

The metabolic biotransformation of shikonin was studied .

紫草素对皮肤无明显刺激性；

No significant skin irritation of shikonin observed .

对紫草素进行温和的磺化处理以提高溶解性能及改善染色性能。

Dyeing performance and solubility of colours could be improved by mild sulfonation method .

紫草素逆转人绒癌耐药细胞JAR/MTX多药耐药性的实验研究

Shikonin Reversal of Drug-resistant Choriocarcinoma Cells JAR / MTX Multi-drug Resistance in Experimental Research

同时固体培养基中的紫草素含量都比液体培养基中的高。

Shikonin content in solid medium was higher than that in liquid medium . 3 .