病毒载体

培养的A-T细胞经pTO-ATM病毒载体转导后，用免疫组织化学方法证明了ATM在这些细胞的异位表达。

Ectopic expression of ATM was demonstrated by immunohistochemistry in cultured A T cells after transduction of the pTO ATM viral vector .

非病毒载体介导的人凝血因子Ⅷ基因在小鼠32D细胞系中的表达

Non Viral Vector Mediating Human Coagulation Factor ⅷ Gene Expression in Mouse 32D Cell Line

E1/E3缺失型腺病毒载体引起细胞周期G2/M阻滞

E1 / E3 Deleted Adenovirus Vectors Induce Cell Cycle G_2 / M Arrest

而基因修饰DC目前最常采用和最有效的方法为腺病毒载体。

At present , the most efficient method for genetic modification of DC is viral vectors .

谷胱甘肽S－转移酶pi基因与逆转录病毒载体的重组体构建

Construction of Recombinant Glutathione S-transferase pi cDNA and Retrovirus Vector

HBSAg及B（7-1）双表达逆转录病毒载体的构建及其在真核细胞中表达

Construction and expression in eukaryotic cells of HBsAg and B_ ( 7-1 ) coexpression retroviral vector

逆转录病毒载体介导双靶区乙型肝炎病毒反义RNA模型的构建

Construction of recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus

目的建立逆转录病毒载体介导的人凝血因子Ⅷ（FⅧ）体外高效表达体系。

Objective To develop a retroviral mediated high efficient expression system of human coagulation factor ⅷ .

含CD基因的腺病毒载体制备及体外抑瘤效果观察

Preparation of an adenovirus expressing cytosine deaminase and observation on its tumor killing effect in vitro

目的构建人bcl-2重组腺病毒载体。

Objective To construct recombinant human Bcl-2 adenoviral vector .

反义人类端粒酶RNA逆转录病毒载体构建及其对结直肠癌细胞的抑制作用

Construction of antisense human telomeric RNA retroviral vector and its inhibition on colorectal cancer cells

目的：研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒（HBV）的作用。

Objective : To study the effects of dual target antisense RNA against HBV infection .

目的研究仙台病毒载体复制序列对未成熟树突状细胞（Dendriticcells，DC）蛋白质表达的影响，为联合运用二者进行基因治疗提供依据。

Objective To investigate the protein change in immature dendritic cells ( DC ) exposed to sendai virus replication sequence .

晚期糖化终产物受体反义RNA腺相关病毒载体的构建及在肾脏系膜细胞中表达

Construction of recombinant RAGE antisense RNA adeno-associated virus vector and its expression in rat mesangial cell

方法用逆转录病毒载体系统将αv、β5基因转染K562细胞，观察αv、β5亚基的表达；

Methods β 5 integrin cDNA was expressed in K562 cells through a retroviral vector system .

用双拷贝逆转录病毒载体转导人G－CSFcDNA的实验研究

A study on improvement of expression of human G-CSF cDNA transferred with retroviral double copy vector

本文主要介绍有可能用于基因工程疫苗的DNA及RNA病毒载体构建及其应用。

Here , we mainly discuss the construction and application of DNA , RNA vectors which can be potentially used for delivery vaccine .

HIV-1重组病毒载体疫苗冻干制剂的制备及其联合免疫效果的研究

Prepare of Lyophilized Recombinant Virus-Based HIV-1 Vaccine and Prime-Boost Strategy

本文采用非复制型脓病毒载体，利用同源重组方法，获得表达G蛋白多肽的非复制型重组腺病毒。

We used the replication - defective adenovirus vector and the homologous recombination , so we obtained the recombinant adenovirus by carrying the G-protein polypeptide .

人B7-1基因的cDNA克隆及其重组腺病毒载体制备

Cloning of human B7-1 gene cDNA and preparation of adenovirus vector carrying B7-1

目的构建人GM-CSF基因的重组腺病毒载体，探讨其在树突状细胞（DC）中的表达。

Objective To construct the recombinant adenovirus vectors containing human GM-CSF gene , and express in dentritic cells .

一DXFD（52）相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建

Partial Sequence Analysis and Construction of Recombinant Retroviral Vec-tor of a DXFD_ ( 52 ) - Related Human Hepatocyte CDNA

HIV-1来源的慢病毒载体转染大鼠脊髓神经干细胞

Lentivirus vector deriving from human immunodeficiency virus-1 transfer to rat spinal cord neural stem cells

腺病毒载体介导的PML生长抑制因子转染人角质形成细胞的实验研究

Experimental Study on the Keratinocytes Transfected by Adenovirus-mediated PML Growth Suppressor

大鼠Nogo受体基因RNA干扰慢病毒载体的构建与鉴定

Construction and identification of a recombinant lentivirus harboring RNAi targeting rat Nogo receptor gene

家蚕核多角体病毒载体稳定高效表达HBeAg条件及其初步应用的研究

Study of the High-Production and the Primary Application of the HBeAg Expressed with BmNPV System

带有CMV、CEA启动子的腺病毒载体介导TK基因在肿瘤细胞中的表达特性

Characteristics of Adenovirus Vector Mediated TK-gene Expression Promoted by CMV and CEA Promoters in Tumor Cells

用杆状病毒载体在家蚕细胞中表达HBeAg基因及其表达产物纯化的研究

Expression of HBeAg Gene with Baculovirus Vector in Silk Worm Cells and its Expression Product Purification

用腺相关病毒载体介导人血管内皮生长因子受体KDR胞外区基因的表达

Expression of Extracellular Domain of VEGF Receptor KDR with Recombinant AAV

假型病毒载体介导tPA基因转导乳鼠心肌成纤维细胞的实验研究

Pseudotyped Retroviral Vector-Mediated Transduction and Expression of Tissue-typed Plasminogen Activator Gene in Neonatal Rat Myocardial Fibroblasts